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Protein

Lysine--tRNA ligase, mitochondrial

Gene

MSK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • lysine-tRNA ligase activity Source: SGD
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • lysyl-tRNA aminoacylation Source: SGD
  • mitochondrial lysyl-tRNA aminoacylation Source: SGD
  • mitochondrial translation Source: SGD
  • tRNA import into mitochondrion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33102-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase, mitochondrial (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:MSK1
Ordered Locus Names:YNL073W
ORF Names:N2364
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL073W.
SGDiS000005017. MSK1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30MitochondrionSequence analysisAdd BLAST30
ChainiPRO_000003581231 – 576Lysine--tRNA ligase, mitochondrialAdd BLAST546

Proteomic databases

MaxQBiP32048.
PRIDEiP32048.

PTM databases

iPTMnetiP32048.

Interactioni

Protein-protein interaction databases

BioGridi35750. 81 interactors.
DIPiDIP-5319N.
IntActiP32048. 1 interactor.
MINTiMINT-518945.

Structurei

3D structure databases

ProteinModelPortaliP32048.
SMRiP32048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000236577.
InParanoidiP32048.
KOiK04567.
OMAiMKYGMPP.
OrthoDBiEOG092C0ZU5.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVLLKRRSL TFAPRWLWCK CRSSRSRPYS LAHAVDTSKM EATRRNGQIV
60 70 80 90 100
KDLGRYYPSM SESALHDLCQ EYKEVTIADF NERFLGNPAT LHHEDNPNLL
110 120 130 140 150
LSINGRIKSI RFSGQKIVFI DLYNGSSGLK NDTQLQLIVN YNKIGGSSED
160 170 180 190 200
KANFSEYMNF LKKGDYIKAL GYPGFSQSRV KMLSLICNKL PIVLSVSQLP
210 220 230 240 250
LPSRLNDETK IKSNRVVDYQ LNGTQTLLVR ARIIKLLRKF LDDRNFVEVE
260 270 280 290 300
TPILSSKSNG AMAKPFITSS KDFDHLELRI APELWLKRLI ISGLQKVYEI
310 320 330 340 350
GKVFRNEGID STHNAEFSTL EFYETYMSMD DIVTRTEDLF KFLITNLQKF
360 370 380 390 400
FQDTRLPVPK TFSELHLALS ENNWKFRKVE FLPTLNKELG IDLMNSGLDI
410 420 430 440 450
NKPSELLKAL PKDIAKKYFP SADNTGQLSS LQILNKLSDV FLEQRHCQST
460 470 480 490 500
LPTVIYHQPA ILSPLAKTDP QNKQVTKRFE VFIKGKEYIN AYEEENCPQL
510 520 530 540 550
QLQKFLQQKQ INELTGNKTE TLSPVIDYQY VETMKYGMPP VGGFGLGIDR
560 570
LCMLFCDKKR IEEVLPFGCV DDVNRQ
Length:576
Mass (Da):66,128
Last modified:February 1, 1996 - v3
Checksum:iE3C440EEF86BB46E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57360 Genomic DNA. Translation: CAA40634.1. Sequence problems.
X86470 Genomic DNA. Translation: CAA60187.1.
Z71349 Genomic DNA. Translation: CAA95947.1.
BK006947 Genomic DNA. Translation: DAA10472.1.
PIRiS14834.
RefSeqiNP_014326.1. NM_001182911.1.

Genome annotation databases

EnsemblFungiiYNL073W; YNL073W; YNL073W.
GeneIDi855651.
KEGGisce:YNL073W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57360 Genomic DNA. Translation: CAA40634.1. Sequence problems.
X86470 Genomic DNA. Translation: CAA60187.1.
Z71349 Genomic DNA. Translation: CAA95947.1.
BK006947 Genomic DNA. Translation: DAA10472.1.
PIRiS14834.
RefSeqiNP_014326.1. NM_001182911.1.

3D structure databases

ProteinModelPortaliP32048.
SMRiP32048.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35750. 81 interactors.
DIPiDIP-5319N.
IntActiP32048. 1 interactor.
MINTiMINT-518945.

PTM databases

iPTMnetiP32048.

Proteomic databases

MaxQBiP32048.
PRIDEiP32048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL073W; YNL073W; YNL073W.
GeneIDi855651.
KEGGisce:YNL073W.

Organism-specific databases

EuPathDBiFungiDB:YNL073W.
SGDiS000005017. MSK1.

Phylogenomic databases

HOGENOMiHOG000236577.
InParanoidiP32048.
KOiK04567.
OMAiMKYGMPP.
OrthoDBiEOG092C0ZU5.

Enzyme and pathway databases

BioCyciYEAST:G3O-33102-MONOMER.

Miscellaneous databases

PROiP32048.

Family and domain databases

CDDicd00775. LysRS_core. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYKM_YEAST
AccessioniPrimary (citable) accession number: P32048
Secondary accession number(s): D6W1A6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.