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Reviewed, UniProtKB/Swiss-Prot P32044 (KPYK_THEAC)

Last modified June 16, 2009. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40
Gene names
Ordered Locus Names: Ta0896
OrganismThermoplasma acidophilum [Complete proteome] [HAMAP]
Taxonomic identifier2303 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma

Protein attributes

Sequence length544 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer.

Sequence similarities

Belongs to the pyruvate kinase family.

Caution

Ref.2 has published some partial sequence, these sequences do not originate from T.acidophilum, rather they seem to be contaminated with human samples.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 544544Pyruvate kinase
PRO_0000112129

Sites

Active site2021 By similarity
Metal binding2041Magnesium By similarity
Metal binding2251Magnesium By similarity
Metal binding2261Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P32044-1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 7BD8BCAF4706A5B2

FASTA54459,147
        10         20         30         40         50         60 
MKTKIVATIG PASSSPEIMK QMIDNGLSLV RINSAHADIK DVSKITQMVR SINRDVGIMI 

        70         80         90        100        110        120 
DLKGPELRTG EFAGGTLKIS SGKDYVMGKD IVLNNMNVLS AVQVGDRILM SDGEVSFEVE 

       130        140        150        160        170        180 
STDPFTIRAL NDGVLRDRSR VNIPGRFIEL GTITDRDRAF IREGIADGVD FFALSFVQKS 

       190        200        210        220        230        240 
ENVDSLRDFV IDSGGDQYII SKIETKSGLD NIEEIVKSSD GIMVARGDLG VELPLKEVVL 

       250        260        270        280        290        300 
AQKHIIKTAH EDGDFTIVAT QVLESMVNNS SPTRAEISDI TNAIIDNADA LMLSEESAIG 

       310        320        330        340        350        360 
KYPVQAVRTL KEVSDYVEDK VSFDSSYYFK GNTIAYSVAR AAKILSDDIK SDGIVALTHT 

       370        380        390        400        410        420 
GSTVRMISSL RPKAMVYAAT VSESLARKLN IYFGVLPLHM EGNAEDLSFS EIMEYIVRSG 

       430        440        450        460        470        480 
RFADGSKLVM TSGDPYFTFG GTNDVKVAVV GKFIGRGYSF GDSLSGTATY GTKGDILMSE 

       490        500        510        520        530        540 
DGRIPGTDFR AFIFTSDIKP SLMSSLKGKT VVTKARLVRQ IKEGERIYID GNTGIILMAS 


PDQK 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
Nature 407:508-513(2000) [PubMed: 11029001] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25905 / AMRC-C165 / DSM 1728 / IFO 15155 / JCM 9062.
[2]"Purification and properties of pyruvate kinase from Thermoplasma acidophilum."
Potter S., Fothergill-Gilmore L.A.
FEMS Microbiol. Lett. 73:235-239(1992) [PubMed: 1426985] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

AL445065 Genomic DNA. Translation: CAC12025.1.
RefSeqNP_394355.1.

3D structure databases

HSSPHSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBaseSearch...

Genome annotation databases

GeneID1456432.
GenomeReviewsGene locus Ta0896 in contig AL139299_GR.
KEGGtac:Ta0896.
NMPDRfig|273075.1.peg.877.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP32044.
OMAP32044. LTFWRDG.

Enzyme and pathway databases

BioCycTACI273075:TA0896-MON.
BRENDA2.7.1.40. 435.

Family and domain databases

InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase_cat.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
[Graphical view]
Gene3DG3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHERPTHR11817. Pyruvate_kinase. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
ProDomPD001009. Pyruvate_kinase. 2 hits.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_THEAC
AccessionPrimary (citable) accession number: P32044
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 1, 2000
Last modified: June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents