P32044 (KPYK_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Ordered Locus Names:

Ta0896

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) [Reference proteome] [HAMAP]

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma › Thermoplasma acidophilum

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the pyruvate kinase family.
Caution	Ref.2 has published some partial sequence, these sequences do not originate from T.acidophilum, rather they seem to be contaminated with human samples.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Pyruvate kinase		PRO_0000112129
Sites
	Metal binding	33	1	Potassium By similarity
	Metal binding	61	1	Potassium By similarity
	Metal binding	204	1	Magnesium By similarity
	Metal binding	228	1	Magnesium By similarity
	Binding site	31	1	Substrate By similarity
	Binding site	227	1	Substrate; via amide nitrogen By similarity
	Binding site	228	1	Substrate; via amide nitrogen By similarity
	Binding site	260	1	Substrate By similarity
	Site	202	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32044 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 7BD8BCAF4706A5B2	FASTA	544	59,147
	10 20 30 40 50 60 MKTKIVATIG PASSSPEIMK QMIDNGLSLV RINSAHADIK DVSKITQMVR SINRDVGIMI 70 80 90 100 110 120 DLKGPELRTG EFAGGTLKIS SGKDYVMGKD IVLNNMNVLS AVQVGDRILM SDGEVSFEVE 130 140 150 160 170 180 STDPFTIRAL NDGVLRDRSR VNIPGRFIEL GTITDRDRAF IREGIADGVD FFALSFVQKS 190 200 210 220 230 240 ENVDSLRDFV IDSGGDQYII SKIETKSGLD NIEEIVKSSD GIMVARGDLG VELPLKEVVL 250 260 270 280 290 300 AQKHIIKTAH EDGDFTIVAT QVLESMVNNS SPTRAEISDI TNAIIDNADA LMLSEESAIG 310 320 330 340 350 360 KYPVQAVRTL KEVSDYVEDK VSFDSSYYFK GNTIAYSVAR AAKILSDDIK SDGIVALTHT 370 380 390 400 410 420 GSTVRMISSL RPKAMVYAAT VSESLARKLN IYFGVLPLHM EGNAEDLSFS EIMEYIVRSG 430 440 450 460 470 480 RFADGSKLVM TSGDPYFTFG GTNDVKVAVV GKFIGRGYSF GDSLSGTATY GTKGDILMSE 490 500 510 520 530 540 DGRIPGTDFR AFIFTSDIKP SLMSSLKGKT VVTKARLVRQ IKEGERIYID GNTGIILMAS PDQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
[2]	"Purification and properties of pyruvate kinase from Thermoplasma acidophilum." Potter S., Fothergill-Gilmore L.A. FEMS Microbiol. Lett. 73:235-239(1992) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445065 Genomic DNA. Translation: CAC12025.1.
RefSeq	NP_394355.1. NC_002578.1.
3D structure databases
ProteinModelPortal	P32044.
ModBase	Search...
Protein-protein interaction databases
STRING	273075.Ta0896.
Proteomic databases
PRIDE	P32044.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC12025; CAC12025; CAC12025.
GeneID	1456432.
KEGG	tac:Ta0896.
Phylogenomic databases
eggNOG	COG0469.
HOGENOM	HOG000021559.
KO	K00873.
OMA	NSGYTAR.
ProtClustDB	PRK05826.
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_THEAC

Accession

Primary (citable) accession number: P32044

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program