Reviewed, UniProtKB/Swiss-Prot P32044 (KPYK_THEAC)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Ordered Locus Names:

Ta0896

Organism

Thermoplasma acidophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2303 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the pyruvate kinase family.
Caution	Ref.2 has published some partial sequence, these sequences do not originate from T.acidophilum, rather they seem to be contaminated with human samples.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Pyruvate kinase		PRO_0000112129
Sites
	Active site	202	1	By similarity
	Metal binding	204	1	Magnesium By similarity
	Metal binding	225	1	Magnesium By similarity
	Metal binding	226	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32044-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 7BD8BCAF4706A5B2	FASTA	544	59,147
	10 20 30 40 50 60 MKTKIVATIG PASSSPEIMK QMIDNGLSLV RINSAHADIK DVSKITQMVR SINRDVGIMI 70 80 90 100 110 120 DLKGPELRTG EFAGGTLKIS SGKDYVMGKD IVLNNMNVLS AVQVGDRILM SDGEVSFEVE 130 140 150 160 170 180 STDPFTIRAL NDGVLRDRSR VNIPGRFIEL GTITDRDRAF IREGIADGVD FFALSFVQKS 190 200 210 220 230 240 ENVDSLRDFV IDSGGDQYII SKIETKSGLD NIEEIVKSSD GIMVARGDLG VELPLKEVVL 250 260 270 280 290 300 AQKHIIKTAH EDGDFTIVAT QVLESMVNNS SPTRAEISDI TNAIIDNADA LMLSEESAIG 310 320 330 340 350 360 KYPVQAVRTL KEVSDYVEDK VSFDSSYYFK GNTIAYSVAR AAKILSDDIK SDGIVALTHT 370 380 390 400 410 420 GSTVRMISSL RPKAMVYAAT VSESLARKLN IYFGVLPLHM EGNAEDLSFS EIMEYIVRSG 430 440 450 460 470 480 RFADGSKLVM TSGDPYFTFG GTNDVKVAVV GKFIGRGYSF GDSLSGTATY GTKGDILMSE 490 500 510 520 530 540 DGRIPGTDFR AFIFTSDIKP SLMSSLKGKT VVTKARLVRQ IKEGERIYID GNTGIILMAS PDQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / AMRC-C165 / DSM 1728 / IFO 15155 / JCM 9062.
[2]	"Purification and properties of pyruvate kinase from Thermoplasma acidophilum." Potter S., Fothergill-Gilmore L.A. FEMS Microbiol. Lett. 73:235-239(1992) [PubMed: 1426985] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL445065 Genomic DNA. Translation: CAC12025.1.
RefSeq	NP_394355.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Genome annotation databases
GeneID	1456432.
GenomeReviews	Gene locus Ta0896 in contig AL139299_GR.
KEGG	tac:Ta0896.
NMPDR	fig|273075.1.peg.877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P32044.
OMA	P32044. LTFWRDG.
Enzyme and pathway databases
BioCyc	TACI273075:TA0896-MON.
BRENDA	2.7.1.40. 435.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_THEAC

Accession

Primary (citable) accession number: P32044

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)