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P32044

- KPYK_THEAC

UniProt

P32044 - KPYK_THEAC

Protein

Pyruvate kinase

Gene

Ta0896

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311SubstrateBy similarity
    Metal bindingi33 – 331PotassiumBy similarity
    Metal bindingi61 – 611PotassiumBy similarity
    Sitei202 – 2021Transition state stabilizerBy similarity
    Metal bindingi204 – 2041MagnesiumBy similarity
    Binding sitei227 – 2271Substrate; via amide nitrogenBy similarity
    Metal bindingi228 – 2281MagnesiumBy similarity
    Binding sitei228 – 2281Substrate; via amide nitrogenBy similarity
    Binding sitei260 – 2601SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Ordered Locus Names:Ta0896
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001024: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 544544Pyruvate kinasePRO_0000112129Add
    BLAST

    Proteomic databases

    PRIDEiP32044.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    STRINGi273075.Ta0896.

    Structurei

    3D structure databases

    ProteinModelPortaliP32044.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    KOiK00873.
    OMAiGTHEEHK.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P32044-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTKIVATIG PASSSPEIMK QMIDNGLSLV RINSAHADIK DVSKITQMVR    50
    SINRDVGIMI DLKGPELRTG EFAGGTLKIS SGKDYVMGKD IVLNNMNVLS 100
    AVQVGDRILM SDGEVSFEVE STDPFTIRAL NDGVLRDRSR VNIPGRFIEL 150
    GTITDRDRAF IREGIADGVD FFALSFVQKS ENVDSLRDFV IDSGGDQYII 200
    SKIETKSGLD NIEEIVKSSD GIMVARGDLG VELPLKEVVL AQKHIIKTAH 250
    EDGDFTIVAT QVLESMVNNS SPTRAEISDI TNAIIDNADA LMLSEESAIG 300
    KYPVQAVRTL KEVSDYVEDK VSFDSSYYFK GNTIAYSVAR AAKILSDDIK 350
    SDGIVALTHT GSTVRMISSL RPKAMVYAAT VSESLARKLN IYFGVLPLHM 400
    EGNAEDLSFS EIMEYIVRSG RFADGSKLVM TSGDPYFTFG GTNDVKVAVV 450
    GKFIGRGYSF GDSLSGTATY GTKGDILMSE DGRIPGTDFR AFIFTSDIKP 500
    SLMSSLKGKT VVTKARLVRQ IKEGERIYID GNTGIILMAS PDQK 544
    Length:544
    Mass (Da):59,147
    Last modified:December 1, 2000 - v2
    Checksum:i7BD8BCAF4706A5B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445065 Genomic DNA. Translation: CAC12025.1.
    RefSeqiNP_394355.1. NC_002578.1.
    WP_010901306.1. NC_002578.1.

    Genome annotation databases

    EnsemblBacteriaiCAC12025; CAC12025; CAC12025.
    GeneIDi1456432.
    KEGGitac:Ta0896.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445065 Genomic DNA. Translation: CAC12025.1 .
    RefSeqi NP_394355.1. NC_002578.1.
    WP_010901306.1. NC_002578.1.

    3D structure databases

    ProteinModelPortali P32044.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273075.Ta0896.

    Proteomic databases

    PRIDEi P32044.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC12025 ; CAC12025 ; CAC12025 .
    GeneIDi 1456432.
    KEGGi tac:Ta0896.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    KOi K00873.
    OMAi GTHEEHK.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
      Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
      Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
    2. "Purification and properties of pyruvate kinase from Thermoplasma acidophilum."
      Potter S., Fothergill-Gilmore L.A.
      FEMS Microbiol. Lett. 73:235-239(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiKPYK_THEAC
    AccessioniPrimary (citable) accession number: P32044
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    PubMed:1426985 has published some partial sequence, these sequences do not originate from T.acidophilum, rather they seem to be contaminated with human samples.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3