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Protein

Complement factor D

Gene

Cfd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Catalytic activityi

Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Charge relay system
Active sitei115 – 1151Charge relay system
Active sitei209 – 2091Charge relay system

GO - Molecular functioni

  1. endopeptidase activity Source: RGD
  2. serine-type endopeptidase activity Source: RGD

GO - Biological processi

  1. complement activation, alternative pathway Source: UniProtKB-KW
  2. Notch signaling pathway Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement alternate pathway, Immunity, Innate immunity

Protein family/group databases

MEROPSiS01.191.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor D (EC:3.4.21.46)
Alternative name(s):
Adipsin
C3 convertase activator
Endogenous vascular elastase
Properdin factor D
Gene namesi
Name:Cfd
Synonyms:Adn, Df
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2498. Cfd.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Propeptidei21 – 255Activation peptide1 PublicationPRO_0000027566
Chaini26 – 263238Complement factor DPRO_0000027567Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...)
Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
Disulfide bondi149 ↔ 215PROSITE-ProRule annotation
Disulfide bondi180 ↔ 196PROSITE-ProRule annotation
Disulfide bondi205 ↔ 230PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP32038.
PRIDEiP32038.

Expressioni

Gene expression databases

GenevestigatoriP32038.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015029.

Structurei

3D structure databases

ProteinModelPortaliP32038.
SMRiP32038. Positions 26-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 254229Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP32038.
KOiK01334.
PhylomeDBiP32038.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSSVYLVAL VVLEAAVCVA QPRGRILGGQ EAMAHARPYM ASVQVNGTHV
60 70 80 90 100
CGGTLVDEQW VLSAAHCMDG VTKDEVVQVL LGAHSLSSPE PYKHLYDVQS
110 120 130 140 150
VVLHPGSRPD SVEDDLMLFK LSHNASLGPH VRPLPLQRED REVKPGTLCD
160 170 180 190 200
VAGWGVVTHA GRRPDVLQQL TVSIMDRNTC NLRTYHDGAI TKNMMCAESN
210 220 230 240 250
RRDTCRGDSG GPLVCGDAVE AVVTWGSRVC GNRRKPGVFT RVATYVPWIE
260
NVLSGNVSVN VTA
Length:263
Mass (Da):28,442
Last modified:November 1, 1997 - v2
Checksum:iD573133568A31CE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73894 mRNA. Translation: AAB31922.1.
PIRiI55608.
RefSeqiNP_001071110.1. NM_001077642.1.
UniGeneiRn.16172.

Genome annotation databases

GeneIDi54249.
KEGGirno:54249.
UCSCiRGD:2498. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73894 mRNA. Translation: AAB31922.1.
PIRiI55608.
RefSeqiNP_001071110.1. NM_001077642.1.
UniGeneiRn.16172.

3D structure databases

ProteinModelPortaliP32038.
SMRiP32038. Positions 26-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015029.

Protein family/group databases

MEROPSiS01.191.

Proteomic databases

PaxDbiP32038.
PRIDEiP32038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi54249.
KEGGirno:54249.
UCSCiRGD:2498. rat.

Organism-specific databases

CTDi1675.
RGDi2498. Cfd.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP32038.
KOiK01334.
PhylomeDBiP32038.

Miscellaneous databases

NextBioi610740.
PROiP32038.

Gene expression databases

GenevestigatoriP32038.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The endogenous vascular elastase that governs development and progression of monocrotaline-induced pulmonary hypertension in rats is a novel enzyme related to the serine proteinase adipsin."
    Zhu L., Wigle D., Hinek A., Kobayashi J., Ye C., Zuker M., Dodo H., Keeley F.W., Rabinovitch M.
    J. Clin. Invest. 94:1163-1171(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Purification and partial characterization of rat factor D."
    Baker B.C., Campbell C.J., Grinham C.J., Turcatti G.
    Biochem. J. 279:775-779(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-55.

Entry informationi

Entry nameiCFAD_RAT
AccessioniPrimary (citable) accession number: P32038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.