Reviewed, UniProtKB/Swiss-Prot P32038 (CFAD_RAT)

Names and origin

Protein names

Recommended name:
    Complement factor D
    EC=3.4.21.46
Alternative name(s):
    C3 convertase activator
    Properdin factor D
    Adipsin
    Endogenous vascular elastase

Gene names

Name:	Cfd
Synonyms:	Adn, Df

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Catalytic activity	Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.
Subcellular location	Secreted.
Post-translational modification	N-glycosylated.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Complement alternate pathway Immune response Innate immunity
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from mutant phenotype. Source: RGD complement activation, alternative pathway Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from direct assay. Source: RGD
Molecular function	serine-type endopeptidase activity Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 25	5	Activation peptide Ref.2		PRO_0000027566
	Chain	26 – 263	238	Complement factor D		PRO_0000027567
Regions
	Domain	26 – 254	229	Peptidase S1
Sites
	Active site	66	1	Charge relay system
	Active site	115	1	Charge relay system
	Active site	209	1	Charge relay system
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...)
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	149 ↔ 215		By similarity
	Disulfide bond	180 ↔ 196		By similarity
	Disulfide bond	205 ↔ 230		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32038-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: D573133568A31CE0	FASTA	263	28,442
	10 20 30 40 50 60 MHSSVYLVAL VVLEAAVCVA QPRGRILGGQ EAMAHARPYM ASVQVNGTHV CGGTLVDEQW 70 80 90 100 110 120 VLSAAHCMDG VTKDEVVQVL LGAHSLSSPE PYKHLYDVQS VVLHPGSRPD SVEDDLMLFK 130 140 150 160 170 180 LSHNASLGPH VRPLPLQRED REVKPGTLCD VAGWGVVTHA GRRPDVLQQL TVSIMDRNTC 190 200 210 220 230 240 NLRTYHDGAI TKNMMCAESN RRDTCRGDSG GPLVCGDAVE AVVTWGSRVC GNRRKPGVFT 250 260 RVATYVPWIE NVLSGNVSVN VTA

References

[1]	"The endogenous vascular elastase that governs development and progression of monocrotaline-induced pulmonary hypertension in rats is a novel enzyme related to the serine proteinase adipsin." Zhu L., Wigle D., Hinek A., Kobayashi J., Ye C., Zuker M., Dodo H., Keeley F.W., Rabinovitch M. J. Clin. Invest. 94:1163-1171(1994) [PubMed: 8083356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Purification and partial characterization of rat factor D." Baker B.C., Campbell C.J., Grinham C.J., Turcatti G. Biochem. J. 279:775-779(1991) [PubMed: 1953671] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-55.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S73894 mRNA. Translation: AAB31922.1.
IPI	IPI00212480.
PIR	I55608.
RefSeq	NP_001071110.1.
UniGene	Rn.16172
3D structure databases
HSSP	HSSP built from PDB template 1FDP based on UniProtKB P00746.
SMR	P32038. Positions 26-254.
ModBase	Search...
Protein family/group databases
MEROPS	S01.191.
Genome annotation databases
Ensembl	ENSRNOG00000033564. Rattus norvegicus. [Contig view]
GeneID	54249.
KEGG	rno:54249.
Organism-specific databases
RGD	2498. Cfd.
Phylogenomic databases
HOVERGEN	P32038.
Enzyme and pathway databases
BRENDA	3.4.21.46. 248.
Gene expression databases
ArrayExpress	P32038.
GermOnline	ENSRNOG00000033564. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	610740.

Entry information

Entry name

CFAD_RAT

Accession

Primary (citable) accession number: P32038

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)