P32038 (CFAD_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Complement factor D
EC=3.4.21.46

Alternative name(s):
Adipsin
C3 convertase activator
Endogenous vascular elastase
Properdin factor D

Gene names

Name:	Cfd
Synonyms:	Adn, Df

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Catalytic activity	Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.
Subcellular location	Secreted.
Post-translational modification	N-glycosylated.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Complement alternate pathway Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Inferred from mutant phenotype. Source: RGD complement activation, alternative pathway Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from direct assay. Source: RGD
Molecular function	serine-type endopeptidase activity Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 25	5	Activation peptide		PRO_0000027566
	Chain	26 – 263	238	Complement factor D		PRO_0000027567
Regions
	Domain	26 – 254	229	Peptidase S1
Sites
	Active site	66	1	Charge relay system
	Active site	115	1	Charge relay system
	Active site	209	1	Charge relay system
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...)
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	149 ↔ 215		By similarity
	Disulfide bond	180 ↔ 196		By similarity
	Disulfide bond	205 ↔ 230		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32038 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: D573133568A31CE0	FASTA	263	28,442
	10 20 30 40 50 60 MHSSVYLVAL VVLEAAVCVA QPRGRILGGQ EAMAHARPYM ASVQVNGTHV CGGTLVDEQW 70 80 90 100 110 120 VLSAAHCMDG VTKDEVVQVL LGAHSLSSPE PYKHLYDVQS VVLHPGSRPD SVEDDLMLFK 130 140 150 160 170 180 LSHNASLGPH VRPLPLQRED REVKPGTLCD VAGWGVVTHA GRRPDVLQQL TVSIMDRNTC 190 200 210 220 230 240 NLRTYHDGAI TKNMMCAESN RRDTCRGDSG GPLVCGDAVE AVVTWGSRVC GNRRKPGVFT 250 260 RVATYVPWIE NVLSGNVSVN VTA

References

[1]	"The endogenous vascular elastase that governs development and progression of monocrotaline-induced pulmonary hypertension in rats is a novel enzyme related to the serine proteinase adipsin." Zhu L., Wigle D., Hinek A., Kobayashi J., Ye C., Zuker M., Dodo H., Keeley F.W., Rabinovitch M. J. Clin. Invest. 94:1163-1171(1994) [PubMed: 8083356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Purification and partial characterization of rat factor D." Baker B.C., Campbell C.J., Grinham C.J., Turcatti G. Biochem. J. 279:775-779(1991) [PubMed: 1953671] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-55.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S73894 mRNA. Translation: AAB31922.1.
IPI	IPI00212480.
PIR	I55608.
RefSeq	NP_001071110.1. NM_001077642.1.
UniGene	Rn.16172.
3D structure databases
ProteinModelPortal	P32038.
SMR	P32038. Positions 26-254.
ModBase	Search...
Protein-protein interaction databases
STRING	P32038.
Protein family/group databases
MEROPS	S01.191.
Proteomic databases
PRIDE	P32038.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	54249.
KEGG	rno:54249.
UCSC	S73894. rat.
Organism-specific databases
CTD	1675.
RGD	2498. Cfd.
Phylogenomic databases
eggNOG	roNOG16853.
GeneTree	ENSGT00580000081367.
HOVERGEN	HBG013304.
InParanoid	P32038.
OrthoDB	EOG4FXR89.
Gene expression databases
ArrayExpress	P32038.
Genevestigator	P32038.
GermOnline	ENSRNOG00000033564. Rattus norvegicus.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01334.
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	610740.

Entry information

Entry name

CFAD_RAT

Accession

Primary (citable) accession number: P32038

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program