P32031 (SLP2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fork head domain transcription factor slp2

Alternative name(s):
Sloppy paired locus protein 2

Gene names

Name:	slp2
ORF Names:	CG2939

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcription factor involved in segmentation. May function primarily as a segment polarity gene. Different levels of slp activity seem to be required in different segments.
Subcellular location	Nucleus.
Tissue specificity	Expressed in the posterior half of each parasegment just anterior to the parasegmental boundary.
Developmental stage	Expression at 3-6 hours of embryogenesis. Strong re-expression in first-instar larvae.
Sequence similarities	Contains 1 fork-head DNA-binding domain.
Sequence caution	The sequence AAF51057.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Developmental protein Segmentation polarity protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Uncategorized	promoter binding Inferred from Biological aspect of Ancestor. Source: RefGenome specific RNA polymerase II transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome specific transcriptional repressor activity Inferred from Biological aspect of Ancestor. Source: RefGenome transcription activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Biological process	axon midline choice point recognition Inferred from Biological aspect of Ancestor. Source: RefGenome dorsal/ventral pattern formation Inferred from Biological aspect of Ancestor. Source: RefGenome embryo development ending in birth or egg hatching Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of neuron differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome neuron fate specification Inferred from Biological aspect of Ancestor. Source: RefGenome nonmotile primary cilium assembly Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of cell cycle Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of neuroblast proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of mitotic cell cycle Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome segment polarity determination Inferred from electronic annotation. Source: UniProtKB-KW specification of segmental identity, head Inferred from Biological aspect of Ancestor. Source: RefGenome tissue development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular component	transcription factor complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	DNA binding, bending Inferred from Biological aspect of Ancestor. Source: RefGenome double-stranded DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific distal enhancer binding RNA polymerase II transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome transcription factor binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Fork head domain transcription factor slp2		PRO_0000091915
Regions
	DNA binding	179 – 270	92	Fork-head
	Compositional bias	94 – 104	11	Asn-rich
Natural variations
	Natural variant	362	1	R → P in strain: Oregon-R.
	Natural variant	378	1	S → N in strain: Oregon-R.
	Natural variant	387	1	R → Q in strain: Canton-S.
	Natural variant	410	1	H → Q in strain: Oregon-R.
	Natural variant	416	1	T → I in strain: Oregon-R.
Experimental info
	Sequence conflict	413	1	P → L in CAA46892. Ref.1
	Sequence conflict	413	1	P → L in CAA46891. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32031 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 3D9744F413585C8D	FASTA	445	50,660
	10 20 30 40 50 60 MVKIEEGLPS SEISAHSLHF QHHHHPLPPT THHSALQSPH PVGLNLTNLM KMARTPHLKS 70 80 90 100 110 120 SFSINSILPE TVEHHDEDEE EDVEKKSPAK FPPNHNNNNL NTTNWGSPED HEAESDPESD 130 140 150 160 170 180 LDVTSMSPAP VANPNESDPD EVDEEFVEED IECDGETTDG DAENKSNDGK PVKDKKGNEK 190 200 210 220 230 240 PPYSYNALIM MAIRQSSEKR LTLNGIYEYI MTNHPYYRDN KQGWQNSIRH NLSLNKCFVK 250 260 270 280 290 300 VPRHYDDPGK GNYWMLDPSA EDVFIGGSTG KLRRRTTAAS RSRLAAFKRS LIGPMFPGLA 310 320 330 340 350 360 AYPQFGQFLT YPPTAPSLLA SMYQRYNPFA PKGGPGHPGL PPGLRDYPDH RDLKDRQDHH 370 380 390 400 410 420 HRPSSHHPRA VSSTSDCSTN SCCINMRQPP LWRLIKGNSR WRQHRRRHNH RPPITTHTWR 430 440 WVRRPFRLAA IPRDPRHSLF TNPSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila sloppy paired locus encodes two proteins involved in segmentation that show homology to mammalian transcription factors." Grossniklaus U., Pearson R.K., Gehring W.J. Genes Dev. 6:1030-1051(1992) [PubMed: 1317319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: Canton-S and Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66098 Genomic DNA. Translation: CAA46892.1. X66097 mRNA. Translation: CAA46891.1. AE014134 Genomic DNA. Translation: AAF51057.1. Sequence problems.
PIR	S23055. S23056.
RefSeq	NP_476834.1. NM_057486.3.
UniGene	Dm.3388.
3D structure databases
ProteinModelPortal	P32031.
SMR	P32031. Positions 180-268.
ModBase	Search...
Protein-protein interaction databases
STRING	P32031.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	33608.
KEGG	dme:Dmel_CG2939.
Organism-specific databases
CTD	33608.
FlyBase	FBgn0004567. slp2.
Phylogenomic databases
InParanoid	P32031.
OrthoDB	EOG4Q575K.
Gene expression databases
Bgee	P32031.
Family and domain databases
InterPro	IPR001766. TF_fork_head. IPR018122. TF_fork_head_CS. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K09385.
Pfam	PF00250. Fork_head. 1 hit. [Graphical view]
PRINTS	PR00053. FORKHEAD.
SMART	SM00339. FH. 1 hit. [Graphical view]
PROSITE	PS00657. FORK_HEAD_1. 1 hit. PS00658. FORK_HEAD_2. 1 hit. PS50039. FORK_HEAD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	784423.

Entry information

Entry name

SLP2_DROME

Accession

Primary (citable) accession number: P32031
Secondary accession number(s): Q9VQV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 2, 2001
Last modified:	January 25, 2012
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project