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Protein

Defensin-like protein

Gene

FST

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in floral organogenesis. May play a protective role in flowers by protecting the reproductive organs from potential pathogen attack.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Protein family/group databases

TCDBi1.C.45.1.2. the plant defensin (plant defensin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-like protein
Alternative name(s):
Flower-specific gamma-thionin
Gene namesi
Name:FST
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 10580Defensin-like proteinPRO_0000007057Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 721 Publication
Disulfide bondi39 ↔ 591 Publication
Disulfide bondi45 ↔ 661 Publication
Disulfide bondi49 ↔ 681 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Flower. Found in petals, stamen and pistils, but not in sepals. In particular, accumulation in a configuration surrounding the inner reproductive whorls.

Developmental stagei

Accumulates in developing flowers and its level drops as flowers mature.

Structurei

Secondary structure

1
105
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 314Combined sources
Helixi42 – 5110Combined sources
Beta strandi55 – 595Combined sources
Beta strandi63 – 708Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MR4NMR-A26-72[»]
ProteinModelPortaliP32026.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32026.

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PRINTSiPR00288. PUROTHIONIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32026-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARSLCFMAF AILAMMLFVA YEVQARECKT ESNTFPGICI TKPPCRKACI
60 70 80 90 100
SEKFTDGHCS KLLRRCLCTK PCVFDEKMIK TGAETLVEEA KTLAAALLEE

EIMDN
Length:105
Mass (Da):11,750
Last modified:July 1, 1993 - v1
Checksum:iDA7F4511F0651BC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11748 mRNA. Translation: CAA77806.1.
PIRiS23574.
RefSeqiXP_016515032.1. XM_016659546.1.

Genome annotation databases

GeneIDi107831752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11748 mRNA. Translation: CAA77806.1.
PIRiS23574.
RefSeqiXP_016515032.1. XM_016659546.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MR4NMR-A26-72[»]
ProteinModelPortaliP32026.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.45.1.2. the plant defensin (plant defensin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107831752.

Miscellaneous databases

EvolutionaryTraceiP32026.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PRINTSiPR00288. PUROTHIONIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A flower-specific cDNA encoding a novel thionin in tobacco."
    Gu Q., Kawata E.E., Morse M.-J., Wu H.-M., Cheung A.Y.
    Mol. Gen. Genet. 234:89-96(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Wisconsin 38.
    Tissue: Flower bud.
  2. "The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of the crop defense protein alfAFP."
    Lay F.T., Schirra H.J., Scanlon M.J., Anderson M.A., Craik D.J.
    J. Mol. Biol. 325:175-188(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 26-72, DISULFIDE BONDS.

Entry informationi

Entry nameiDEF_TOBAC
AccessioniPrimary (citable) accession number: P32026
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 6, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was initially thought to be a thionin.1 Publication

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.