P32023 (KGP25_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cGMP-dependent protein kinase, isozyme 2 forms cD5/T2
Short name=cGK
EC=2.7.11.12

Alternative name(s):
Foraging protein

Gene names

Name:	for
Synonyms:	DG2, PGK2, Pkg24A
ORF Names:	CG10033

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	934 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Developmental stage	Expressed throughout development, high during embryonic and adult stages. Isoform T2 is predominantly expressed during larval and adult stages. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	habituation Inferred from mutant phenotype PubMed 11040266. Source: FlyBase larval feeding behavior Inferred from mutant phenotype PubMed 89278147. Source: UniProtKB larval locomotory behavior Inferred from mutant phenotype PubMed 8506349. Source: FlyBase long-term memory Inferred from mutant phenotype PubMed 17640898. Source: FlyBase regulation of heart contraction Inferred from mutant phenotype PubMed 11919704. Source: FlyBase regulation of response to food Inferred from mutant phenotype PubMed 19696884. Source: FlyBase response to sucrose stimulus Inferred from direct assay PubMed 17701979. Source: FlyBase short-term memory Inferred from mutant phenotype PubMed 17640898. Source: FlyBase
Cellular_component	plasma membrane Inferred from direct assay PubMed 15218025. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP-dependent protein kinase activity Inferred from direct assay PubMed 89278147. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform cD5 (identifier: P32023-1)

Also known as: E; J;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform T2 (identifier: P32023-2)

Also known as: C; D; F; G; T2a; T2b;

The sequence of this isoform differs from the canonical sequence as follows:
     368-407: Missing.

Isoform cD4 (identifier: Q03043-4)

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform T1 (identifier: Q03043-1)

Also known as: A; H;

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform T3A (identifier: Q03043-2)

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform T3B (identifier: Q03043-3)

Also known as: B;

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 934	934	cGMP-dependent protein kinase, isozyme 2 forms cD5/T2		PRO_0000086122
Regions
	Domain	623 – 882	260	Protein kinase
	Domain	883 – 934	52	AGC-kinase C-terminal
	Nucleotide binding	409 – 491	83	cGMP 1 (truncated) By similarity
	Nucleotide binding	492 – 607	116	cGMP 2 By similarity
	Nucleotide binding	629 – 637	9	ATP By similarity
Sites
	Active site	747	1	Proton acceptor By similarity
	Binding site	653	1	ATP By similarity
Natural variations
	Alternative sequence	368 – 407	40	Missing in isoform T2.		VSP_004764
Experimental info
	Sequence conflict	66	1	A → V in AAA28454. Ref.1
	Sequence conflict	219	1	Q → H in AAA28456. Ref.1
	Sequence conflict	219	1	Q → H in AAA28457. Ref.1
	Sequence conflict	219	1	Q → H in AAA28458. Ref.1
	Sequence conflict	393	1	N → H in AAA28454. Ref.1
	Sequence conflict	401	1	N → D in AAA28454. Ref.1
	Sequence conflict	404 – 406	3	IFS → NFF in AAA28454. Ref.1
	Sequence conflict	464	1	M → L in AAA28454. Ref.1
	Sequence conflict	510	1	Y → H in AAA28456. Ref.1
	Sequence conflict	510	1	Y → H in AAA28458. Ref.1
	Sequence conflict	761	1	V → G in AAA28454. Ref.1
	Sequence conflict	761	1	V → G in AAA28456. Ref.1
	Sequence conflict	761	1	V → G in AAA28457. Ref.1
	Sequence conflict	761	1	V → G in AAA28458. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform cD5 (E) (J) [UniParc]. Last modified February 1, 2005. Version 3. Checksum: 5087411147CD5CCA	FASTA	934	105,907
	10 20 30 40 50 60 MKIKHYPGKA VDASLSLEGS SAMGALYEAN WLRAANQPAA PATTGTKLSR QSSSAGSSFL 70 80 90 100 110 120 IEGISALSKY QMTLENIRQL ELQSRDKRIA STIKELSGYR PSALQHHQQQ QMHNVWVAED 130 140 150 160 170 180 QDQEHEELED ASEGKEKLAS IQEPPAVNHY VLDPTERPRV PRPRQQFSVK PPSLRRSQTM 190 200 210 220 230 240 SQPPSYATLR SPPKIKENLS KSSSAYSTFS SAAEDSQDQV VICQQPQRLM APPPREPPPE 250 260 270 280 290 300 PPKRVSKPLS RSQTSVQRYA TVRMPNQTTS FSRSVVRSRD STASQRRLSL EQAIEGLKLE 310 320 330 340 350 360 GEKAVRQKSP QISPAASSNG SSKDLNGEGF CIPRPRLIVP VHTYARRRRT GNLKEQSSGG 370 380 390 400 410 420 QEEEAEKGKG WKDFYVLSQD RHSSFYINRI GQNYDYDYPI NFNIFSPDGR VEVSREGKYL 430 440 450 460 470 480 STLSGAKVLG ELAILYNCQR TATITAITEC NLWAIERQCF QTIMMRTGLI RQAEYSDFLK 490 500 510 520 530 540 SVPIFKDLAE DTLIKISDVL EETHYQRGDY IVRQGARGDT FFIISKGKVR VTIKQQDTQE 550 560 570 580 590 600 EKFIRMLGKG DFFGEKALQG DDLRTANIIC ESADGVSCLV IDRETFNQLI SNLDEIKHRY 610 620 630 640 650 660 DDEGAMERRK INEEFRDINL TDLRVIATLG VGGFGRVELV QTNGDSSRSF ALKQMKKSQI 670 680 690 700 710 720 VETRQQQHIM SEKEIMGEAN CQFIVKLFKT FKDKKYLYML MESCLGGELW TILRDKGNFD 730 740 750 760 770 780 DSTTRFYTAC VVEAFDYLHS RNIIYRDLKP ENLLLNERGY VKLVDFGFAK KLQTGRKTWT 790 800 810 820 830 840 FCGTPEYVAP EVILNRGHDI SADYWSLGVL MFELLTGTPP FTGSDPMRTY NIILKGIDAI 850 860 870 880 890 900 EFPRNITRNA SNLIKKLCRD NPAERLGYQR GGISEIQKHK WFDGFYWWGL QNCTLEPPIK 910 920 930 PAVKSVVDTT NFDDYPPDPE GPPPDDVTGW DKDF
	Isoform T2 (C) (D) (F) (G) (T2a) (T2b) [UniParc]. Checksum: 13E8CF5A5A506981	FASTA	894	101,058
	10 20 30 40 50 60 MKIKHYPGKA VDASLSLEGS SAMGALYEAN WLRAANQPAA PATTGTKLSR QSSSAGSSFL 70 80 90 100 110 120 IEGISALSKY QMTLENIRQL ELQSRDKRIA STIKELSGYR PSALQHHQQQ QMHNVWVAED 130 140 150 160 170 180 QDQEHEELED ASEGKEKLAS IQEPPAVNHY VLDPTERPRV PRPRQQFSVK PPSLRRSQTM 190 200 210 220 230 240 SQPPSYATLR SPPKIKENLS KSSSAYSTFS SAAEDSQDQV VICQQPQRLM APPPREPPPE 250 260 270 280 290 300 PPKRVSKPLS RSQTSVQRYA TVRMPNQTTS FSRSVVRSRD STASQRRLSL EQAIEGLKLE 310 320 330 340 350 360 GEKAVRQKSP QISPAASSNG SSKDLNGEGF CIPRPRLIVP VHTYARRRRT GNLKEQSSGG 370 380 390 400 410 420 QEEEAEKDGR VEVSREGKYL STLSGAKVLG ELAILYNCQR TATITAITEC NLWAIERQCF 430 440 450 460 470 480 QTIMMRTGLI RQAEYSDFLK SVPIFKDLAE DTLIKISDVL EETHYQRGDY IVRQGARGDT 490 500 510 520 530 540 FFIISKGKVR VTIKQQDTQE EKFIRMLGKG DFFGEKALQG DDLRTANIIC ESADGVSCLV 550 560 570 580 590 600 IDRETFNQLI SNLDEIKHRY DDEGAMERRK INEEFRDINL TDLRVIATLG VGGFGRVELV 610 620 630 640 650 660 QTNGDSSRSF ALKQMKKSQI VETRQQQHIM SEKEIMGEAN CQFIVKLFKT FKDKKYLYML 670 680 690 700 710 720 MESCLGGELW TILRDKGNFD DSTTRFYTAC VVEAFDYLHS RNIIYRDLKP ENLLLNERGY 730 740 750 760 770 780 VKLVDFGFAK KLQTGRKTWT FCGTPEYVAP EVILNRGHDI SADYWSLGVL MFELLTGTPP 790 800 810 820 830 840 FTGSDPMRTY NIILKGIDAI EFPRNITRNA SNLIKKLCRD NPAERLGYQR GGISEIQKHK 850 860 870 880 890 WFDGFYWWGL QNCTLEPPIK PAVKSVVDTT NFDDYPPDPE GPPPDDVTGW DKDF
	Isoform cD4 [UniParc]. See Q03043.		–	–
	Isoform T1 (A) (H) [UniParc]. See Q03043.		–	–
	Isoform T3A [UniParc]. See Q03043.		–	–
	Isoform T3B (B) [UniParc]. See Q03043.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cGMP-dependent protein kinase genes in Drosophila." Kalderon D., Rubin G.M. J. Biol. Chem. 264:10738-10748(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CD5 AND T2), DEVELOPMENTAL STAGE. Strain: Canton-S. Tissue: Imaginal disk.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T2). Strain: Berkeley. Tissue: Embryo and Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27124, M27122, M27123 Genomic DNA. Translation: AAA28457.1. M30147 mRNA. Translation: AAA28456.1. M30148 mRNA. Translation: AAA28458.1. M30149 mRNA. Translation: AAA28454.1. AE014134 Genomic DNA. Translation: AAG22252.2. AE014134 Genomic DNA. Translation: AAG22254.1. AE014134 Genomic DNA. Translation: AAS64615.1. AE014134 Genomic DNA. Translation: AAS64616.1. AE014134 Genomic DNA. Translation: AAX52650.1. AF132175 mRNA. Translation: AAD34763.2. BT004881 mRNA. Translation: AAO45237.1.
PIR	C34106. D34106. T08418.
RefSeq	NP_001014464.1. NM_001014464.1. NP_001162858.1. NM_001169387.1. NP_477489.1. NM_058141.3. NP_477490.1. NM_058142.3. NP_599146.1. NM_134319.3. NP_995627.1. NM_205905.1. NP_995629.1. NM_205907.1.
UniGene	Dm.6584.
3D structure databases
ProteinModelPortal	P32023.
SMR	P32023. Positions 364-918.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089306; FBpp0088352; FBgn0000721. FBtr0100363; FBpp0099769; FBgn0000721.
GeneID	44817.
KEGG	dme:Dmel_CG10033.
UCSC	CG10033-RA. d. melanogaster.
Organism-specific databases
CTD	44817.
FlyBase	FBgn0000721. for.
Phylogenomic databases
GeneTree	ENSGT00550000074358.
InParanoid	P32023.
KO	K07376.
OMA	DMRTANI.
Enzyme and pathway databases
BRENDA	2.7.11.12. 1994.
Gene expression databases
Bgee	P32023.
GermOnline	CG10033. Drosophila melanogaster.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
InterPro	IPR000961. AGC-kinase_C. IPR002374. cGMP_dep_kinase. IPR018490. cNMP-bd-like. IPR018488. cNMP-bd_CS. IPR000595. cNMP-bd_dom. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR014710. RmlC-like_jellyroll. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR00104. CGMPKINASE.
SMART	SM00100. cNMP. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 2 hits. SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. 1 hit. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	44817.
NextBio	837662.

Entry information

Entry name

KGP25_DROME

Accession

Primary (citable) accession number: P32023
Secondary accession number(s): A4V044 , A4V046, Q0E8U1, Q24302, Q24303, Q9I7Q0, Q9I7Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 129 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project