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P32023

- KGP25_DROME

UniProt

P32023 - KGP25_DROME

Protein

cGMP-dependent protein kinase, isozyme 2 forms cD5/T2

Gene

for

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 3 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei653 – 6531ATPPROSITE-ProRule annotation
    Active sitei747 – 7471Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi409 – 49183cGMP 1 (truncated)By similarityAdd
    BLAST
    Nucleotide bindingi492 – 607116cGMP 2By similarityAdd
    BLAST
    Nucleotide bindingi629 – 6379ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cGMP binding Source: UniProtKB-KW
    3. cGMP-dependent protein kinase activity Source: UniProtKB
    4. cyclic nucleotide-dependent protein kinase activity Source: FlyBase
    5. protein serine/threonine kinase activity Source: FlyBase

    GO - Biological processi

    1. feeding behavior Source: FlyBase
    2. habituation Source: FlyBase
    3. larval feeding behavior Source: UniProtKB
    4. larval locomotory behavior Source: FlyBase
    5. long-term memory Source: FlyBase
    6. protein phosphorylation Source: UniProtKB
    7. regulation of heart contraction Source: FlyBase
    8. regulation of response to food Source: FlyBase
    9. response to sucrose Source: FlyBase
    10. short-term memory Source: FlyBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, cGMP, cGMP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.12. 1994.
    ReactomeiREACT_184321. Ca2+ pathway.
    SignaLinkiP32023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-dependent protein kinase, isozyme 2 forms cD5/T2 (EC:2.7.11.12)
    Short name:
    cGK
    Alternative name(s):
    Foraging protein
    Gene namesi
    Name:for
    Synonyms:DG2, PGK2, Pkg24A
    ORF Names:CG10033
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0000721. for.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 934934cGMP-dependent protein kinase, isozyme 2 forms cD5/T2PRO_0000086122Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed throughout development, high during embryonic and adult stages. Isoform T2 is predominantly expressed during larval and adult stages.1 Publication

    Gene expression databases

    BgeeiP32023.

    Interactioni

    Protein-protein interaction databases

    BioGridi69303. 9 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP32023.
    SMRiP32023. Positions 364-918.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini623 – 882260Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini883 – 93452AGC-kinase C-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00550000074358.
    InParanoidiP32023.
    KOiK07376.
    OMAiDMRTANI.

    Family and domain databases

    Gene3Di2.60.120.10. 2 hits.
    InterProiIPR000961. AGC-kinase_C.
    IPR002374. cGMP_dep_kinase.
    IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR014710. RmlC-like_jellyroll.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR00104. CGMPKINASE.
    SMARTiSM00100. cNMP. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 2 hits.
    PS50042. CNMP_BINDING_3. 2 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform cD5 (identifier: P32023-1) [UniParc]FASTAAdd to Basket

    Also known as: E, J

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKIKHYPGKA VDASLSLEGS SAMGALYEAN WLRAANQPAA PATTGTKLSR    50
    QSSSAGSSFL IEGISALSKY QMTLENIRQL ELQSRDKRIA STIKELSGYR 100
    PSALQHHQQQ QMHNVWVAED QDQEHEELED ASEGKEKLAS IQEPPAVNHY 150
    VLDPTERPRV PRPRQQFSVK PPSLRRSQTM SQPPSYATLR SPPKIKENLS 200
    KSSSAYSTFS SAAEDSQDQV VICQQPQRLM APPPREPPPE PPKRVSKPLS 250
    RSQTSVQRYA TVRMPNQTTS FSRSVVRSRD STASQRRLSL EQAIEGLKLE 300
    GEKAVRQKSP QISPAASSNG SSKDLNGEGF CIPRPRLIVP VHTYARRRRT 350
    GNLKEQSSGG QEEEAEKGKG WKDFYVLSQD RHSSFYINRI GQNYDYDYPI 400
    NFNIFSPDGR VEVSREGKYL STLSGAKVLG ELAILYNCQR TATITAITEC 450
    NLWAIERQCF QTIMMRTGLI RQAEYSDFLK SVPIFKDLAE DTLIKISDVL 500
    EETHYQRGDY IVRQGARGDT FFIISKGKVR VTIKQQDTQE EKFIRMLGKG 550
    DFFGEKALQG DDLRTANIIC ESADGVSCLV IDRETFNQLI SNLDEIKHRY 600
    DDEGAMERRK INEEFRDINL TDLRVIATLG VGGFGRVELV QTNGDSSRSF 650
    ALKQMKKSQI VETRQQQHIM SEKEIMGEAN CQFIVKLFKT FKDKKYLYML 700
    MESCLGGELW TILRDKGNFD DSTTRFYTAC VVEAFDYLHS RNIIYRDLKP 750
    ENLLLNERGY VKLVDFGFAK KLQTGRKTWT FCGTPEYVAP EVILNRGHDI 800
    SADYWSLGVL MFELLTGTPP FTGSDPMRTY NIILKGIDAI EFPRNITRNA 850
    SNLIKKLCRD NPAERLGYQR GGISEIQKHK WFDGFYWWGL QNCTLEPPIK 900
    PAVKSVVDTT NFDDYPPDPE GPPPDDVTGW DKDF 934
    Length:934
    Mass (Da):105,907
    Last modified:February 1, 2005 - v3
    Checksum:i5087411147CD5CCA
    GO
    Isoform T2 (identifier: P32023-2) [UniParc]FASTAAdd to Basket

    Also known as: C, D, F, G, T2a, T2b

    The sequence of this isoform differs from the canonical sequence as follows:
         368-407: Missing.

    Show »
    Length:894
    Mass (Da):101,058
    Checksum:i13E8CF5A5A506981
    GO
    Isoform cD4 (identifier: Q03043-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q03043.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:568
    Mass (Da):65,073
    GO
    Isoform T1 (identifier: Q03043-1) [UniParc]FASTAAdd to Basket

    Also known as: A, H

    The sequence of this isoform can be found in the external entry Q03043.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:1,088
    Mass (Da):121,341
    GO
    Isoform T3A (identifier: Q03043-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q03043.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:919
    Mass (Da):102,235
    GO
    Isoform T3B (identifier: Q03043-3) [UniParc]FASTAAdd to Basket

    Also known as: B

    The sequence of this isoform can be found in the external entry Q03043.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:742
    Mass (Da):83,349
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661A → V in AAA28454. (PubMed:2732245)Curated
    Sequence conflicti219 – 2191Q → H in AAA28456. (PubMed:2732245)Curated
    Sequence conflicti219 – 2191Q → H in AAA28457. (PubMed:2732245)Curated
    Sequence conflicti219 – 2191Q → H in AAA28458. (PubMed:2732245)Curated
    Sequence conflicti393 – 3931N → H in AAA28454. (PubMed:2732245)Curated
    Sequence conflicti401 – 4011N → D in AAA28454. (PubMed:2732245)Curated
    Sequence conflicti404 – 4063IFS → NFF in AAA28454. (PubMed:2732245)Curated
    Sequence conflicti464 – 4641M → L in AAA28454. (PubMed:2732245)Curated
    Sequence conflicti510 – 5101Y → H in AAA28456. (PubMed:2732245)Curated
    Sequence conflicti510 – 5101Y → H in AAA28458. (PubMed:2732245)Curated
    Sequence conflicti761 – 7611V → G in AAA28454. (PubMed:2732245)Curated
    Sequence conflicti761 – 7611V → G in AAA28456. (PubMed:2732245)Curated
    Sequence conflicti761 – 7611V → G in AAA28457. (PubMed:2732245)Curated
    Sequence conflicti761 – 7611V → G in AAA28458. (PubMed:2732245)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei368 – 40740Missing in isoform T2. 2 PublicationsVSP_004764Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27124, M27122, M27123 Genomic DNA. Translation: AAA28457.1.
    M30147 mRNA. Translation: AAA28456.1.
    M30148 mRNA. Translation: AAA28458.1.
    M30149 mRNA. Translation: AAA28454.1.
    AE014134 Genomic DNA. Translation: AAG22252.2.
    AE014134 Genomic DNA. Translation: AAG22254.1.
    AE014134 Genomic DNA. Translation: AAS64615.1.
    AE014134 Genomic DNA. Translation: AAS64616.1.
    AE014134 Genomic DNA. Translation: AAX52650.1.
    AF132175 mRNA. Translation: AAD34763.2.
    BT004881 mRNA. Translation: AAO45237.1.
    PIRiC34106.
    D34106.
    T08418.
    RefSeqiNP_001014464.1. NM_001014464.1. [P32023-1]
    NP_001162858.1. NM_001169387.1. [P32023-2]
    NP_477489.1. NM_058141.3. [P32023-2]
    NP_477490.1. NM_058142.3. [P32023-1]
    NP_599146.1. NM_134319.3. [P32023-2]
    NP_995627.1. NM_205905.1. [P32023-2]
    NP_995629.1. NM_205907.1. [P32023-2]
    UniGeneiDm.23542.
    Dm.6584.

    Genome annotation databases

    EnsemblMetazoaiFBtr0089306; FBpp0088352; FBgn0000721. [P32023-1]
    FBtr0100363; FBpp0099769; FBgn0000721. [P32023-1]
    GeneIDi44817.
    KEGGidme:Dmel_CG10033.
    UCSCiCG10033-RA. d. melanogaster. [P32023-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27124 , M27122 , M27123 Genomic DNA. Translation: AAA28457.1 .
    M30147 mRNA. Translation: AAA28456.1 .
    M30148 mRNA. Translation: AAA28458.1 .
    M30149 mRNA. Translation: AAA28454.1 .
    AE014134 Genomic DNA. Translation: AAG22252.2 .
    AE014134 Genomic DNA. Translation: AAG22254.1 .
    AE014134 Genomic DNA. Translation: AAS64615.1 .
    AE014134 Genomic DNA. Translation: AAS64616.1 .
    AE014134 Genomic DNA. Translation: AAX52650.1 .
    AF132175 mRNA. Translation: AAD34763.2 .
    BT004881 mRNA. Translation: AAO45237.1 .
    PIRi C34106.
    D34106.
    T08418.
    RefSeqi NP_001014464.1. NM_001014464.1. [P32023-1 ]
    NP_001162858.1. NM_001169387.1. [P32023-2 ]
    NP_477489.1. NM_058141.3. [P32023-2 ]
    NP_477490.1. NM_058142.3. [P32023-1 ]
    NP_599146.1. NM_134319.3. [P32023-2 ]
    NP_995627.1. NM_205905.1. [P32023-2 ]
    NP_995629.1. NM_205907.1. [P32023-2 ]
    UniGenei Dm.23542.
    Dm.6584.

    3D structure databases

    ProteinModelPortali P32023.
    SMRi P32023. Positions 364-918.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 69303. 9 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0089306 ; FBpp0088352 ; FBgn0000721 . [P32023-1 ]
    FBtr0100363 ; FBpp0099769 ; FBgn0000721 . [P32023-1 ]
    GeneIDi 44817.
    KEGGi dme:Dmel_CG10033.
    UCSCi CG10033-RA. d. melanogaster. [P32023-1 ]

    Organism-specific databases

    CTDi 44817.
    FlyBasei FBgn0000721. for.

    Phylogenomic databases

    GeneTreei ENSGT00550000074358.
    InParanoidi P32023.
    KOi K07376.
    OMAi DMRTANI.

    Enzyme and pathway databases

    BRENDAi 2.7.11.12. 1994.
    Reactomei REACT_184321. Ca2+ pathway.
    SignaLinki P32023.

    Miscellaneous databases

    GenomeRNAii 44817.
    NextBioi 837662.

    Gene expression databases

    Bgeei P32023.

    Family and domain databases

    Gene3Di 2.60.120.10. 2 hits.
    InterProi IPR000961. AGC-kinase_C.
    IPR002374. cGMP_dep_kinase.
    IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR014710. RmlC-like_jellyroll.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 2 hits.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR00104. CGMPKINASE.
    SMARTi SM00100. cNMP. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 2 hits.
    PS50042. CNMP_BINDING_3. 2 hits.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cGMP-dependent protein kinase genes in Drosophila."
      Kalderon D., Rubin G.M.
      J. Biol. Chem. 264:10738-10748(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CD5 AND T2), DEVELOPMENTAL STAGE.
      Strain: Canton-S.
      Tissue: Imaginal disk.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T2).
      Strain: Berkeley.
      Tissue: Embryo and Head.

    Entry informationi

    Entry nameiKGP25_DROME
    AccessioniPrimary (citable) accession number: P32023
    Secondary accession number(s): A4V044
    , A4V046, Q0E8U1, Q24302, Q24303, Q9I7Q0, Q9I7Q2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 141 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3