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Protein

cGMP-dependent protein kinase, isozyme 2 forms cD5/T2

Gene

for

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei653 – 6531ATPPROSITE-ProRule annotation
Active sitei747 – 7471Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi409 – 49183cGMP 1 (truncated)By similarityAdd
BLAST
Nucleotide bindingi492 – 607116cGMP 2By similarityAdd
BLAST
Nucleotide bindingi629 – 6379ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cGMP binding Source: UniProtKB-KW
  3. cGMP-dependent protein kinase activity Source: FlyBase
  4. cyclic nucleotide-dependent protein kinase activity Source: FlyBase
  5. protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  1. feeding behavior Source: FlyBase
  2. habituation Source: FlyBase
  3. larval feeding behavior Source: UniProtKB
  4. larval locomotory behavior Source: FlyBase
  5. long-term memory Source: FlyBase
  6. protein phosphorylation Source: UniProtKB
  7. regulation of heart contraction Source: FlyBase
  8. regulation of response to food Source: FlyBase
  9. response to sucrose Source: FlyBase
  10. short-term memory Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.12. 1994.
ReactomeiREACT_310517. Rap1 signalling.
REACT_325910. cGMP effects.
SignaLinkiP32023.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-dependent protein kinase, isozyme 2 forms cD5/T2 (EC:2.7.11.12)
Short name:
cGK
Alternative name(s):
Foraging protein
Gene namesi
Name:for
Synonyms:DG2, PGK2, Pkg24A
ORF Names:CG10033
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000721. for.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934cGMP-dependent protein kinase, isozyme 2 forms cD5/T2PRO_0000086122Add
BLAST

Expressioni

Developmental stagei

Expressed throughout development, high during embryonic and adult stages. Isoform T2 is predominantly expressed during larval and adult stages.1 Publication

Gene expression databases

BgeeiP32023.
ExpressionAtlasiP32023. differential.

Interactioni

Protein-protein interaction databases

BioGridi69303. 9 interactions.

Structurei

3D structure databases

ProteinModelPortaliP32023.
SMRiP32023. Positions 364-934.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini623 – 882260Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini883 – 93452AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00780000121885.
KOiK07376.
OMAiDMRTANI.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00104. CGMPKINASE.
SMARTiSM00100. cNMP. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform cD5 (identifier: P32023-1) [UniParc]FASTAAdd to basket

Also known as: E, J

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIKHYPGKA VDASLSLEGS SAMGALYEAN WLRAANQPAA PATTGTKLSR
60 70 80 90 100
QSSSAGSSFL IEGISALSKY QMTLENIRQL ELQSRDKRIA STIKELSGYR
110 120 130 140 150
PSALQHHQQQ QMHNVWVAED QDQEHEELED ASEGKEKLAS IQEPPAVNHY
160 170 180 190 200
VLDPTERPRV PRPRQQFSVK PPSLRRSQTM SQPPSYATLR SPPKIKENLS
210 220 230 240 250
KSSSAYSTFS SAAEDSQDQV VICQQPQRLM APPPREPPPE PPKRVSKPLS
260 270 280 290 300
RSQTSVQRYA TVRMPNQTTS FSRSVVRSRD STASQRRLSL EQAIEGLKLE
310 320 330 340 350
GEKAVRQKSP QISPAASSNG SSKDLNGEGF CIPRPRLIVP VHTYARRRRT
360 370 380 390 400
GNLKEQSSGG QEEEAEKGKG WKDFYVLSQD RHSSFYINRI GQNYDYDYPI
410 420 430 440 450
NFNIFSPDGR VEVSREGKYL STLSGAKVLG ELAILYNCQR TATITAITEC
460 470 480 490 500
NLWAIERQCF QTIMMRTGLI RQAEYSDFLK SVPIFKDLAE DTLIKISDVL
510 520 530 540 550
EETHYQRGDY IVRQGARGDT FFIISKGKVR VTIKQQDTQE EKFIRMLGKG
560 570 580 590 600
DFFGEKALQG DDLRTANIIC ESADGVSCLV IDRETFNQLI SNLDEIKHRY
610 620 630 640 650
DDEGAMERRK INEEFRDINL TDLRVIATLG VGGFGRVELV QTNGDSSRSF
660 670 680 690 700
ALKQMKKSQI VETRQQQHIM SEKEIMGEAN CQFIVKLFKT FKDKKYLYML
710 720 730 740 750
MESCLGGELW TILRDKGNFD DSTTRFYTAC VVEAFDYLHS RNIIYRDLKP
760 770 780 790 800
ENLLLNERGY VKLVDFGFAK KLQTGRKTWT FCGTPEYVAP EVILNRGHDI
810 820 830 840 850
SADYWSLGVL MFELLTGTPP FTGSDPMRTY NIILKGIDAI EFPRNITRNA
860 870 880 890 900
SNLIKKLCRD NPAERLGYQR GGISEIQKHK WFDGFYWWGL QNCTLEPPIK
910 920 930
PAVKSVVDTT NFDDYPPDPE GPPPDDVTGW DKDF
Length:934
Mass (Da):105,907
Last modified:January 31, 2005 - v3
Checksum:i5087411147CD5CCA
GO
Isoform T2 (identifier: P32023-2) [UniParc]FASTAAdd to basket

Also known as: C, D, F, G, T2a, T2b

The sequence of this isoform differs from the canonical sequence as follows:
     368-407: Missing.

Show »
Length:894
Mass (Da):101,058
Checksum:i13E8CF5A5A506981
GO
Isoform cD4 (identifier: Q03043-4) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:568
Mass (Da):65,073
GO
Isoform T1 (identifier: Q03043-1) [UniParc]FASTAAdd to basket

Also known as: A, H

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,088
Mass (Da):121,341
GO
Isoform T3A (identifier: Q03043-2) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:919
Mass (Da):102,235
GO
Isoform T3B (identifier: Q03043-3) [UniParc]FASTAAdd to basket

Also known as: B

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:742
Mass (Da):83,349
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661A → V in AAA28454 (PubMed:2732245).Curated
Sequence conflicti219 – 2191Q → H in AAA28456 (PubMed:2732245).Curated
Sequence conflicti219 – 2191Q → H in AAA28457 (PubMed:2732245).Curated
Sequence conflicti219 – 2191Q → H in AAA28458 (PubMed:2732245).Curated
Sequence conflicti393 – 3931N → H in AAA28454 (PubMed:2732245).Curated
Sequence conflicti401 – 4011N → D in AAA28454 (PubMed:2732245).Curated
Sequence conflicti404 – 4063IFS → NFF in AAA28454 (PubMed:2732245).Curated
Sequence conflicti464 – 4641M → L in AAA28454 (PubMed:2732245).Curated
Sequence conflicti510 – 5101Y → H in AAA28456 (PubMed:2732245).Curated
Sequence conflicti510 – 5101Y → H in AAA28458 (PubMed:2732245).Curated
Sequence conflicti761 – 7611V → G in AAA28454 (PubMed:2732245).Curated
Sequence conflicti761 – 7611V → G in AAA28456 (PubMed:2732245).Curated
Sequence conflicti761 – 7611V → G in AAA28457 (PubMed:2732245).Curated
Sequence conflicti761 – 7611V → G in AAA28458 (PubMed:2732245).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei368 – 40740Missing in isoform T2. 2 PublicationsVSP_004764Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27124, M27122, M27123 Genomic DNA. Translation: AAA28457.1.
M30147 mRNA. Translation: AAA28456.1.
M30148 mRNA. Translation: AAA28458.1.
M30149 mRNA. Translation: AAA28454.1.
AE014134 Genomic DNA. Translation: AAG22252.2.
AE014134 Genomic DNA. Translation: AAG22254.1.
AE014134 Genomic DNA. Translation: AAS64615.1.
AE014134 Genomic DNA. Translation: AAS64616.1.
AE014134 Genomic DNA. Translation: AAX52650.1.
AF132175 mRNA. Translation: AAD34763.2.
BT004881 mRNA. Translation: AAO45237.1.
PIRiC34106.
D34106.
T08418.
RefSeqiNP_001014464.1. NM_001014464.2. [P32023-1]
NP_001162858.1. NM_001169387.2. [P32023-2]
NP_477489.1. NM_058141.4. [P32023-2]
NP_477490.1. NM_058142.4. [P32023-1]
NP_599146.1. NM_134319.4. [P32023-2]
NP_995629.1. NM_205907.2. [P32023-2]
UniGeneiDm.6584.

Genome annotation databases

EnsemblMetazoaiFBtr0089306; FBpp0088352; FBgn0000721. [P32023-1]
FBtr0100363; FBpp0099769; FBgn0000721. [P32023-1]
GeneIDi44817.
KEGGidme:Dmel_CG10033.
UCSCiCG10033-RA. d. melanogaster. [P32023-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27124, M27122, M27123 Genomic DNA. Translation: AAA28457.1.
M30147 mRNA. Translation: AAA28456.1.
M30148 mRNA. Translation: AAA28458.1.
M30149 mRNA. Translation: AAA28454.1.
AE014134 Genomic DNA. Translation: AAG22252.2.
AE014134 Genomic DNA. Translation: AAG22254.1.
AE014134 Genomic DNA. Translation: AAS64615.1.
AE014134 Genomic DNA. Translation: AAS64616.1.
AE014134 Genomic DNA. Translation: AAX52650.1.
AF132175 mRNA. Translation: AAD34763.2.
BT004881 mRNA. Translation: AAO45237.1.
PIRiC34106.
D34106.
T08418.
RefSeqiNP_001014464.1. NM_001014464.2. [P32023-1]
NP_001162858.1. NM_001169387.2. [P32023-2]
NP_477489.1. NM_058141.4. [P32023-2]
NP_477490.1. NM_058142.4. [P32023-1]
NP_599146.1. NM_134319.4. [P32023-2]
NP_995629.1. NM_205907.2. [P32023-2]
UniGeneiDm.6584.

3D structure databases

ProteinModelPortaliP32023.
SMRiP32023. Positions 364-934.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69303. 9 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089306; FBpp0088352; FBgn0000721. [P32023-1]
FBtr0100363; FBpp0099769; FBgn0000721. [P32023-1]
GeneIDi44817.
KEGGidme:Dmel_CG10033.
UCSCiCG10033-RA. d. melanogaster. [P32023-1]

Organism-specific databases

CTDi44817.
FlyBaseiFBgn0000721. for.

Phylogenomic databases

GeneTreeiENSGT00780000121885.
KOiK07376.
OMAiDMRTANI.

Enzyme and pathway databases

BRENDAi2.7.11.12. 1994.
ReactomeiREACT_310517. Rap1 signalling.
REACT_325910. cGMP effects.
SignaLinkiP32023.

Miscellaneous databases

GenomeRNAii44817.
NextBioi837662.

Gene expression databases

BgeeiP32023.
ExpressionAtlasiP32023. differential.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00104. CGMPKINASE.
SMARTiSM00100. cNMP. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cGMP-dependent protein kinase genes in Drosophila."
    Kalderon D., Rubin G.M.
    J. Biol. Chem. 264:10738-10748(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CD5 AND T2), DEVELOPMENTAL STAGE.
    Strain: Canton-S.
    Tissue: Imaginal disk.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T2).
    Strain: Berkeley.
    Tissue: Embryo and Head.

Entry informationi

Entry nameiKGP25_DROME
AccessioniPrimary (citable) accession number: P32023
Secondary accession number(s): A4V044
, A4V046, Q0E8U1, Q24302, Q24303, Q9I7Q0, Q9I7Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 1993
Last sequence update: January 31, 2005
Last modified: March 31, 2015
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.