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P32023 (KGP25_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cGMP-dependent protein kinase, isozyme 2 forms cD5/T2

Short name=cGK
EC=2.7.11.12
Alternative name(s):
Foraging protein
Gene names
Name:for
Synonyms:DG2, PGK2, Pkg24A
ORF Names:CG10033
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length934 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Developmental stage

Expressed throughout development, high during embryonic and adult stages. Isoform T2 is predominantly expressed during larval and adult stages. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 2 cyclic nucleotide-binding domains.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandATP-binding
cGMP
cGMP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfeeding behavior

Traceable author statement PubMed 11715043. Source: FlyBase

habituation

Inferred from mutant phenotype PubMed 11040266. Source: FlyBase

larval feeding behavior

Inferred from mutant phenotype PubMed 89278147. Source: UniProtKB

larval locomotory behavior

Inferred from mutant phenotype PubMed 8506349. Source: FlyBase

long-term memory

Inferred from mutant phenotype PubMed 17640898. Source: FlyBase

protein phosphorylation

Inferred from direct assay PubMed 89278147. Source: UniProtKB

regulation of heart contraction

Inferred from mutant phenotype PubMed 11919704. Source: FlyBase

regulation of response to food

Inferred from mutant phenotype PubMed 19696884. Source: FlyBase

response to sucrose

Inferred from direct assay PubMed 17701979. Source: FlyBase

short-term memory

Inferred from mutant phenotype PubMed 17640898. Source: FlyBase

   Cellular_componentplasma membrane

Inferred from direct assay PubMed 15218025. Source: FlyBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cGMP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cGMP-dependent protein kinase activity

Inferred from direct assay PubMed 89278147. Source: UniProtKB

cyclic nucleotide-dependent protein kinase activity

Inferred from sequence or structural similarity PubMed 3215511. Source: FlyBase

protein serine/threonine kinase activity

Non-traceable author statement PubMed 10908587. Source: FlyBase

Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform cD5 (identifier: P32023-1)

Also known as: E; J;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform T2 (identifier: P32023-2)

Also known as: C; D; F; G; T2a; T2b;

The sequence of this isoform differs from the canonical sequence as follows:
     368-407: Missing.
Isoform cD4 (identifier: Q03043-4)

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform T1 (identifier: Q03043-1)

Also known as: A; H;

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform T3A (identifier: Q03043-2)

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform T3B (identifier: Q03043-3)

Also known as: B;

The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 934934cGMP-dependent protein kinase, isozyme 2 forms cD5/T2
PRO_0000086122

Regions

Domain623 – 882260Protein kinase
Domain883 – 93452AGC-kinase C-terminal
Nucleotide binding409 – 49183cGMP 1 (truncated) By similarity
Nucleotide binding492 – 607116cGMP 2 By similarity
Nucleotide binding629 – 6379ATP By similarity

Sites

Active site7471Proton acceptor By similarity
Binding site6531ATP By similarity

Natural variations

Alternative sequence368 – 40740Missing in isoform T2.
VSP_004764

Experimental info

Sequence conflict661A → V in AAA28454. Ref.1
Sequence conflict2191Q → H in AAA28456. Ref.1
Sequence conflict2191Q → H in AAA28457. Ref.1
Sequence conflict2191Q → H in AAA28458. Ref.1
Sequence conflict3931N → H in AAA28454. Ref.1
Sequence conflict4011N → D in AAA28454. Ref.1
Sequence conflict404 – 4063IFS → NFF in AAA28454. Ref.1
Sequence conflict4641M → L in AAA28454. Ref.1
Sequence conflict5101Y → H in AAA28456. Ref.1
Sequence conflict5101Y → H in AAA28458. Ref.1
Sequence conflict7611V → G in AAA28454. Ref.1
Sequence conflict7611V → G in AAA28456. Ref.1
Sequence conflict7611V → G in AAA28457. Ref.1
Sequence conflict7611V → G in AAA28458. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform cD5 (E) (J) [UniParc].

Last modified February 1, 2005. Version 3.
Checksum: 5087411147CD5CCA

FASTA934105,907
        10         20         30         40         50         60 
MKIKHYPGKA VDASLSLEGS SAMGALYEAN WLRAANQPAA PATTGTKLSR QSSSAGSSFL 

        70         80         90        100        110        120 
IEGISALSKY QMTLENIRQL ELQSRDKRIA STIKELSGYR PSALQHHQQQ QMHNVWVAED 

       130        140        150        160        170        180 
QDQEHEELED ASEGKEKLAS IQEPPAVNHY VLDPTERPRV PRPRQQFSVK PPSLRRSQTM 

       190        200        210        220        230        240 
SQPPSYATLR SPPKIKENLS KSSSAYSTFS SAAEDSQDQV VICQQPQRLM APPPREPPPE 

       250        260        270        280        290        300 
PPKRVSKPLS RSQTSVQRYA TVRMPNQTTS FSRSVVRSRD STASQRRLSL EQAIEGLKLE 

       310        320        330        340        350        360 
GEKAVRQKSP QISPAASSNG SSKDLNGEGF CIPRPRLIVP VHTYARRRRT GNLKEQSSGG 

       370        380        390        400        410        420 
QEEEAEKGKG WKDFYVLSQD RHSSFYINRI GQNYDYDYPI NFNIFSPDGR VEVSREGKYL 

       430        440        450        460        470        480 
STLSGAKVLG ELAILYNCQR TATITAITEC NLWAIERQCF QTIMMRTGLI RQAEYSDFLK 

       490        500        510        520        530        540 
SVPIFKDLAE DTLIKISDVL EETHYQRGDY IVRQGARGDT FFIISKGKVR VTIKQQDTQE 

       550        560        570        580        590        600 
EKFIRMLGKG DFFGEKALQG DDLRTANIIC ESADGVSCLV IDRETFNQLI SNLDEIKHRY 

       610        620        630        640        650        660 
DDEGAMERRK INEEFRDINL TDLRVIATLG VGGFGRVELV QTNGDSSRSF ALKQMKKSQI 

       670        680        690        700        710        720 
VETRQQQHIM SEKEIMGEAN CQFIVKLFKT FKDKKYLYML MESCLGGELW TILRDKGNFD 

       730        740        750        760        770        780 
DSTTRFYTAC VVEAFDYLHS RNIIYRDLKP ENLLLNERGY VKLVDFGFAK KLQTGRKTWT 

       790        800        810        820        830        840 
FCGTPEYVAP EVILNRGHDI SADYWSLGVL MFELLTGTPP FTGSDPMRTY NIILKGIDAI 

       850        860        870        880        890        900 
EFPRNITRNA SNLIKKLCRD NPAERLGYQR GGISEIQKHK WFDGFYWWGL QNCTLEPPIK 

       910        920        930 
PAVKSVVDTT NFDDYPPDPE GPPPDDVTGW DKDF 

« Hide

Isoform T2 (C) (D) (F) (G) (T2a) (T2b) [UniParc].

Checksum: 13E8CF5A5A506981
Show »

FASTA894101,058
Isoform cD4 [UniParc].

See Q03043.

Isoform T1 (A) (H) [UniParc].

See Q03043.

Isoform T3A [UniParc].

See Q03043.

Isoform T3B (B) [UniParc].

See Q03043.

References

« Hide 'large scale' references
[1]"cGMP-dependent protein kinase genes in Drosophila."
Kalderon D., Rubin G.M.
J. Biol. Chem. 264:10738-10748(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS CD5 AND T2), DEVELOPMENTAL STAGE.
Strain: Canton-S.
Tissue: Imaginal disk.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T2).
Strain: Berkeley.
Tissue: Embryo and Head.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27124, M27122, M27123 Genomic DNA. Translation: AAA28457.1.
M30147 mRNA. Translation: AAA28456.1.
M30148 mRNA. Translation: AAA28458.1.
M30149 mRNA. Translation: AAA28454.1.
AE014134 Genomic DNA. Translation: AAG22252.2.
AE014134 Genomic DNA. Translation: AAG22254.1.
AE014134 Genomic DNA. Translation: AAS64615.1.
AE014134 Genomic DNA. Translation: AAS64616.1.
AE014134 Genomic DNA. Translation: AAX52650.1.
AF132175 mRNA. Translation: AAD34763.2.
BT004881 mRNA. Translation: AAO45237.1.
PIRC34106.
D34106.
T08418.
RefSeqNP_001014464.1. NM_001014464.1. [P32023-1]
NP_001162858.1. NM_001169387.1. [P32023-2]
NP_477489.1. NM_058141.3. [P32023-2]
NP_477490.1. NM_058142.3. [P32023-1]
NP_599146.1. NM_134319.3. [P32023-2]
NP_995627.1. NM_205905.1. [P32023-2]
NP_995629.1. NM_205907.1. [P32023-2]
UniGeneDm.23542.
Dm.6584.

3D structure databases

ProteinModelPortalP32023.
SMRP32023. Positions 364-918.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid69303. 9 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0089306; FBpp0088352; FBgn0000721. [P32023-1]
FBtr0100363; FBpp0099769; FBgn0000721. [P32023-1]
GeneID44817.
KEGGdme:Dmel_CG10033.
UCSCCG10033-RA. d. melanogaster. [P32023-1]

Organism-specific databases

CTD44817.
FlyBaseFBgn0000721. for.

Phylogenomic databases

GeneTreeENSGT00550000074358.
InParanoidP32023.
KOK07376.
OMADMRTANI.

Enzyme and pathway databases

BRENDA2.7.11.12. 1994.
SignaLinkP32023.

Gene expression databases

BgeeP32023.

Family and domain databases

Gene3D2.60.120.10. 2 hits.
InterProIPR000961. AGC-kinase_C.
IPR002374. cGMP_dep_kinase.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014710. RmlC-like_jellyroll.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00027. cNMP_binding. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR00104. CGMPKINASE.
SMARTSM00100. cNMP. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi44817.
NextBio837662.

Entry information

Entry nameKGP25_DROME
AccessionPrimary (citable) accession number: P32023
Secondary accession number(s): A4V044 expand/collapse secondary AC list , A4V046, Q0E8U1, Q24302, Q24303, Q9I7Q0, Q9I7Q2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2005
Last modified: July 9, 2014
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase