Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

cGMP-dependent protein kinase, isozyme 2 forms cD5/T2

Gene

for

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei545cGMP 2By similarity1
Binding sitei600cAMP or cGMP 2By similarity1
Binding sitei653ATPPROSITE-ProRule annotation1
Active sitei747Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi430 – 433cAMP or cGMP 1By similarity4
Nucleotide bindingi440 – 441cAMP or cGMP 1By similarity2
Nucleotide bindingi554 – 557cAMP or cGMP 2By similarity4
Nucleotide bindingi564 – 565cAMP or cGMP 2By similarity2
Nucleotide bindingi629 – 637ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cGMP binding Source: UniProtKB-KW
  • cGMP-dependent protein kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  • feeding behavior Source: FlyBase
  • habituation Source: FlyBase
  • larval feeding behavior Source: UniProtKB
  • larval locomotory behavior Source: FlyBase
  • long-term memory Source: FlyBase
  • motor neuron axon guidance Source: FlyBase
  • protein phosphorylation Source: UniProtKB
  • regulation of BMP signaling pathway Source: FlyBase
  • regulation of heart contraction Source: FlyBase
  • regulation of response to food Source: FlyBase
  • response to sucrose Source: FlyBase
  • short-term memory Source: FlyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, cGMP, cGMP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.12 1994
ReactomeiR-DME-392517 Rap1 signalling
SignaLinkiP32023

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-dependent protein kinase, isozyme 2 forms cD5/T2 (EC:2.7.11.12)
Short name:
cGK
Alternative name(s):
Foraging protein
Gene namesi
Name:for
Synonyms:DG2, PGK2, Pkg24A
ORF Names:CG10033
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000721 for

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861221 – 934cGMP-dependent protein kinase, isozyme 2 forms cD5/T2Add BLAST934

Proteomic databases

PRIDEiP32023

Expressioni

Developmental stagei

Expressed throughout development, high during embryonic and adult stages. Isoform T2 is predominantly expressed during larval and adult stages.1 Publication

Gene expression databases

BgeeiFBgn0000721
ExpressionAtlasiP32023 baseline and differential
GenevisibleiP32023 DM

Interactioni

Protein-protein interaction databases

BioGridi69303, 12 interactors

Structurei

3D structure databases

ProteinModelPortaliP32023
SMRiP32023
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini623 – 882Protein kinasePROSITE-ProRule annotationAdd BLAST260
Domaini883 – 934AGC-kinase C-terminalAdd BLAST52

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0616 Eukaryota
ENOG410XPQQ LUCA
GeneTreeiENSGT00810000125385
KOiK07376
OMAiHNGWIAE
OrthoDBiEOG091G0S9R

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
cd05572 STKc_cGK, 1 hit
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR002374 cGMP_dep_kinase
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR014710 RmlC-like_jellyroll
IPR008271 Ser/Thr_kinase_AS
IPR035014 STKc_cGK
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF00069 Pkinase, 1 hit
PRINTSiPR00104 CGMPKINASE
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00888 CNMP_BINDING_1, 1 hit
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform cD5 (identifier: P32023-1) [UniParc]FASTAAdd to basket
Also known as: E, J

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKIKHYPGKA VDASLSLEGS SAMGALYEAN WLRAANQPAA PATTGTKLSR
60 70 80 90 100
QSSSAGSSFL IEGISALSKY QMTLENIRQL ELQSRDKRIA STIKELSGYR
110 120 130 140 150
PSALQHHQQQ QMHNVWVAED QDQEHEELED ASEGKEKLAS IQEPPAVNHY
160 170 180 190 200
VLDPTERPRV PRPRQQFSVK PPSLRRSQTM SQPPSYATLR SPPKIKENLS
210 220 230 240 250
KSSSAYSTFS SAAEDSQDQV VICQQPQRLM APPPREPPPE PPKRVSKPLS
260 270 280 290 300
RSQTSVQRYA TVRMPNQTTS FSRSVVRSRD STASQRRLSL EQAIEGLKLE
310 320 330 340 350
GEKAVRQKSP QISPAASSNG SSKDLNGEGF CIPRPRLIVP VHTYARRRRT
360 370 380 390 400
GNLKEQSSGG QEEEAEKGKG WKDFYVLSQD RHSSFYINRI GQNYDYDYPI
410 420 430 440 450
NFNIFSPDGR VEVSREGKYL STLSGAKVLG ELAILYNCQR TATITAITEC
460 470 480 490 500
NLWAIERQCF QTIMMRTGLI RQAEYSDFLK SVPIFKDLAE DTLIKISDVL
510 520 530 540 550
EETHYQRGDY IVRQGARGDT FFIISKGKVR VTIKQQDTQE EKFIRMLGKG
560 570 580 590 600
DFFGEKALQG DDLRTANIIC ESADGVSCLV IDRETFNQLI SNLDEIKHRY
610 620 630 640 650
DDEGAMERRK INEEFRDINL TDLRVIATLG VGGFGRVELV QTNGDSSRSF
660 670 680 690 700
ALKQMKKSQI VETRQQQHIM SEKEIMGEAN CQFIVKLFKT FKDKKYLYML
710 720 730 740 750
MESCLGGELW TILRDKGNFD DSTTRFYTAC VVEAFDYLHS RNIIYRDLKP
760 770 780 790 800
ENLLLNERGY VKLVDFGFAK KLQTGRKTWT FCGTPEYVAP EVILNRGHDI
810 820 830 840 850
SADYWSLGVL MFELLTGTPP FTGSDPMRTY NIILKGIDAI EFPRNITRNA
860 870 880 890 900
SNLIKKLCRD NPAERLGYQR GGISEIQKHK WFDGFYWWGL QNCTLEPPIK
910 920 930
PAVKSVVDTT NFDDYPPDPE GPPPDDVTGW DKDF
Length:934
Mass (Da):105,907
Last modified:February 1, 2005 - v3
Checksum:i5087411147CD5CCA
GO
Isoform T2 (identifier: P32023-2) [UniParc]FASTAAdd to basket
Also known as: C, D, G, T2a, T2b

The sequence of this isoform differs from the canonical sequence as follows:
     368-407: Missing.

Show »
Length:894
Mass (Da):101,058
Checksum:i13E8CF5A5A506981
GO
Isoform cD4 (identifier: Q03043-4) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:568
Mass (Da):65,073
GO
Isoform T1 (identifier: Q03043-1) [UniParc]FASTAAdd to basket
Also known as: A, H
The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:1,088
Mass (Da):121,341
GO
Isoform T3A (identifier: Q03043-2) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:919
Mass (Da):102,235
GO
Isoform T3B (identifier: Q03043-3) [UniParc]FASTAAdd to basket
Also known as: B
The sequence of this isoform can be found in the external entry Q03043.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:742
Mass (Da):83,349
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66A → V in AAA28454 (PubMed:2732245).Curated1
Sequence conflicti219Q → H in AAA28456 (PubMed:2732245).Curated1
Sequence conflicti219Q → H in AAA28457 (PubMed:2732245).Curated1
Sequence conflicti219Q → H in AAA28458 (PubMed:2732245).Curated1
Sequence conflicti393N → H in AAA28454 (PubMed:2732245).Curated1
Sequence conflicti401N → D in AAA28454 (PubMed:2732245).Curated1
Sequence conflicti404 – 406IFS → NFF in AAA28454 (PubMed:2732245).Curated3
Sequence conflicti464M → L in AAA28454 (PubMed:2732245).Curated1
Sequence conflicti510Y → H in AAA28456 (PubMed:2732245).Curated1
Sequence conflicti510Y → H in AAA28458 (PubMed:2732245).Curated1
Sequence conflicti761V → G in AAA28454 (PubMed:2732245).Curated1
Sequence conflicti761V → G in AAA28456 (PubMed:2732245).Curated1
Sequence conflicti761V → G in AAA28457 (PubMed:2732245).Curated1
Sequence conflicti761V → G in AAA28458 (PubMed:2732245).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004764368 – 407Missing in isoform T2. 2 PublicationsAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27124, M27122, M27123 Genomic DNA Translation: AAA28457.1
M30147 mRNA Translation: AAA28456.1
M30148 mRNA Translation: AAA28458.1
M30149 mRNA Translation: AAA28454.1
AE014134 Genomic DNA Translation: AAG22252.2
AE014134 Genomic DNA Translation: AAG22254.1
AE014134 Genomic DNA Translation: AAS64616.1
AE014134 Genomic DNA Translation: AAX52650.1
AF132175 mRNA Translation: AAD34763.2
BT004881 mRNA Translation: AAO45237.1
PIRiC34106
D34106
T08418
RefSeqiNP_001014464.1, NM_001014464.2 [P32023-1]
NP_001162858.1, NM_001169387.2 [P32023-2]
NP_477489.1, NM_058141.4 [P32023-2]
NP_477490.1, NM_058142.4 [P32023-1]
NP_599146.1, NM_134319.4 [P32023-2]
NP_995629.1, NM_205907.2 [P32023-2]
UniGeneiDm.6584

Genome annotation databases

EnsemblMetazoaiFBtr0089306; FBpp0088352; FBgn0000721 [P32023-1]
FBtr0089307; FBpp0088353; FBgn0000721 [P32023-2]
FBtr0089308; FBpp0088354; FBgn0000721 [P32023-2]
FBtr0089309; FBpp0088924; FBgn0000721 [P32023-2]
FBtr0100363; FBpp0099769; FBgn0000721 [P32023-1]
FBtr0301399; FBpp0290613; FBgn0000721 [P32023-2]
GeneIDi44817
KEGGidme:Dmel_CG10033
UCSCiCG10033-RA d. melanogaster [P32023-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKGP25_DROME
AccessioniPrimary (citable) accession number: P32023
Secondary accession number(s): A4V044
, A4V046, Q0E8U1, Q24302, Q24303, Q9I7Q0, Q9I7Q2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 174 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health