Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Non-specific lipid-transfer protein

Gene

Scp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. May play a role in regulating steroidogenesis.

Catalytic activityi

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanoyl-CoA + propanoyl-CoA = CoA + 3-alpha,7-alpha,12-alpha-trihydroxy-24-oxo-5-beta-cholestanoyl-CoA.

GO - Molecular functioni

GO - Biological processi

  • acyl-CoA metabolic process Source: MGI
  • cholesterol transport Source: GO_Central
  • lipid hydroperoxide transport Source: MGI
  • peroxisome organization Source: MGI
  • phospholipid transport Source: GO_Central
  • positive regulation of intracellular cholesterol transport Source: MGI
  • steroid biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BRENDAi2.3.1.176. 3474.
ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-2046106. alpha-linolenic acid (ALA) metabolism.
R-MMU-389887. Beta-oxidation of pristanoyl-CoA.

Chemistry databases

SwissLipidsiSLP:000000495.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-specific lipid-transfer protein (EC:2.3.1.176)
Short name:
NSL-TP
Alternative name(s):
Propanoyl-CoA C-acyltransferase
SCP-chi
SCPX
Sterol carrier protein 2
Short name:
SCP-2
Sterol carrier protein X
Short name:
SCP-X
Gene namesi
Name:Scp2
Synonyms:Scp-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:98254. Scp2.

Subcellular locationi

  • Cytoplasm

  • Note: Cytoplasmic in the liver and also associated with mitochondria especially in steroidogenic tissues.
Isoform SCPx :
  • Peroxisome

  • Note: Interaction with PEX5 is essential for peroxisomal import.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000340931 – 547Non-specific lipid-transfer proteinAdd BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineBy similarity1
Modified residuei7N6-acetyllysineCombined sources1
Modified residuei8PhosphoserineCombined sources1
Modified residuei40N6-succinyllysineCombined sources1
Modified residuei132N6-acetyllysine; alternateCombined sources1
Modified residuei132N6-succinyllysine; alternateCombined sources1
Modified residuei168N6-succinyllysineCombined sources1
Modified residuei173N6-acetyllysineCombined sources1
Modified residuei177N6-acetyllysineCombined sources1
Modified residuei183N6-acetyllysine; alternateCombined sources1
Modified residuei183N6-succinyllysine; alternateCombined sources1
Modified residuei211N6-succinyllysineCombined sources1
Modified residuei282N6-succinyllysineCombined sources1
Modified residuei341N6-acetyllysine; alternateCombined sources1
Modified residuei341N6-succinyllysine; alternateCombined sources1
Modified residuei432N6-acetyllysine; alternateCombined sources1
Modified residuei432N6-succinyllysine; alternateCombined sources1
Modified residuei438N6-acetyllysine; alternateCombined sources1
Modified residuei438N6-succinyllysine; alternateCombined sources1
Modified residuei443N6-acetyllysine; alternateCombined sources1
Modified residuei443N6-succinyllysine; alternateCombined sources1
Modified residuei453N6-acetyllysine; alternateCombined sources1
Modified residuei453N6-succinyllysine; alternateCombined sources1
Modified residuei464N6-succinyllysineCombined sources1
Modified residuei470N6-acetyllysine; alternateCombined sources1
Modified residuei470N6-succinyllysine; alternateCombined sources1
Modified residuei479N6-succinyllysineCombined sources1
Modified residuei491N6-acetyllysineCombined sources1
Modified residuei492N6-succinyllysineCombined sources1
Modified residuei511N6-succinyllysineCombined sources1
Modified residuei516PhosphoserineCombined sources1
Modified residuei522N6-succinyllysineCombined sources1
Modified residuei534N6-succinyllysineCombined sources1
Modified residuei544N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP32020.
PaxDbiP32020.
PeptideAtlasiP32020.
PRIDEiP32020.
TopDownProteomicsiP32020-1. [P32020-1]

PTM databases

iPTMnetiP32020.
PhosphoSitePlusiP32020.

Expressioni

Tissue specificityi

Present at low levels in all tissues examined but expressed predominantly in the liver.

Gene expression databases

BgeeiENSMUSG00000028603.
CleanExiMM_SCP2.
ExpressionAtlasiP32020. baseline and differential.
GenevisibleiP32020. MM.

Interactioni

Subunit structurei

Interacts with PEX5.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP32020. 6 interactors.
MINTiMINT-219254.
STRINGi10090.ENSMUSP00000030340.

Structurei

3D structure databases

ProteinModelPortaliP32020.
SMRiP32020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini433 – 543SCP2Add BLAST111

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi545 – 547Microbody targeting signalSequence analysis3

Sequence similaritiesi

In the N-terminal section; belongs to the thiolase family.Curated
Contains 1 SCP2 domain.Curated

Phylogenomic databases

eggNOGiKOG1406. Eukaryota.
KOG4170. Eukaryota.
ENOG410XPRW. LUCA.
GeneTreeiENSGT00530000062928.
HOGENOMiHOG000221741.
HOVERGENiHBG006506.
InParanoidiP32020.
KOiK08764.
OMAiHMEVMIN.
OrthoDBiEOG091G026S.
PhylomeDBiP32020.
TreeFamiTF300574.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.47.10. 4 hits.
InterProiIPR003033. SCP2_sterol-bd_dom.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02036. SCP2. 1 hit.
PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
SSF55718. SSF55718. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform SCPx (identifier: P32020-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPSVALKSPR LRRVFVVGVG MTKFMKPGGE NSRDYPDMAK EAGQKALEDA
60 70 80 90 100
QIPYSAVEQA CVGYVYGDST SGQRAIYHSL GLTGIPIINV NNNCSTGSTA
110 120 130 140 150
LFMAHQLIQG GLANCVLALG FEKMERGSIG TKFSDRTTPT DKHIEVLIDK
160 170 180 190 200
YGLSAHPITP QMFGYAGKEH MEKYGTKVEH FAKIGWKNHK HSVNNTYSQF
210 220 230 240 250
QDEYSLEEVM KSKPVFDFLT ILQCCPTSDG AAAAILSSEE FVQQYGLQSK
260 270 280 290 300
AVEIVAQEMM TDLPSTFEEK SIIKVVGYDM SKEAARRCYE KSGLTPNDVD
310 320 330 340 350
VIELHDCFSV NELITYEALG LCPEGQGGTL VDRGDNTYGG KWVINPSGGL
360 370 380 390 400
ISKGHPLGAT GLAQCAELCW QLRGEAGKRQ VPGAKVALQH NLGLGGAVVV
410 420 430 440 450
TLYRMGFPEA ASSFRTHQVS AAPTSSAGDG FKANLVFKEI EKKLEEEGEQ
460 470 480 490 500
FVKKIGGIFA FKVKDGPGGK EATWVVDVKN GKGSVLPNSD KKADCTITMA
510 520 530 540
DSDLLALMTG KMNPQSAFFQ GKLKIAGNMG LAMKLQNLQL QPGKAKL
Length:547
Mass (Da):59,126
Last modified:June 16, 2003 - v3
Checksum:i37BA2E730D9CB105
GO
Isoform SCP2 (identifier: P32020-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-404: Missing.

Note: Mitochondrial precursor. Contains a mitochondrial transit peptide at positions 405-424 (Potential).Curated
Show »
Length:143
Mass (Da):15,237
Checksum:i6A466BBBFB9B9A00
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232A → C in AAA40098 (PubMed:8428655).Curated1
Sequence conflicti478V → A in AAA40099 (PubMed:1709640).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188951 – 404Missing in isoform SCP2. CuratedAdd BLAST404

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91458 mRNA. Translation: AAA40098.1.
AK002425 mRNA. Translation: BAB22092.2.
AK004860 mRNA. No translation available.
AL844206 Genomic DNA. Translation: CAM27075.1.
AL844206 Genomic DNA. Translation: CAM27076.1.
BC018384 mRNA. Translation: AAH18384.1.
BC034613 mRNA. Translation: AAH34613.1.
M62361 mRNA. Translation: AAA40099.1.
X91150 Genomic DNA. Translation: CAA62592.1.
CCDSiCCDS18446.1. [P32020-1]
PIRiA40015.
JU0157.
RefSeqiNP_035457.1. NM_011327.4. [P32020-1]
UniGeneiMm.379011.

Genome annotation databases

EnsembliENSMUST00000030340; ENSMUSP00000030340; ENSMUSG00000028603. [P32020-1]
ENSMUST00000106701; ENSMUSP00000102312; ENSMUSG00000028603. [P32020-2]
GeneIDi20280.
KEGGimmu:20280.
UCSCiuc008uas.2. mouse. [P32020-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91458 mRNA. Translation: AAA40098.1.
AK002425 mRNA. Translation: BAB22092.2.
AK004860 mRNA. No translation available.
AL844206 Genomic DNA. Translation: CAM27075.1.
AL844206 Genomic DNA. Translation: CAM27076.1.
BC018384 mRNA. Translation: AAH18384.1.
BC034613 mRNA. Translation: AAH34613.1.
M62361 mRNA. Translation: AAA40099.1.
X91150 Genomic DNA. Translation: CAA62592.1.
CCDSiCCDS18446.1. [P32020-1]
PIRiA40015.
JU0157.
RefSeqiNP_035457.1. NM_011327.4. [P32020-1]
UniGeneiMm.379011.

3D structure databases

ProteinModelPortaliP32020.
SMRiP32020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP32020. 6 interactors.
MINTiMINT-219254.
STRINGi10090.ENSMUSP00000030340.

Chemistry databases

SwissLipidsiSLP:000000495.

PTM databases

iPTMnetiP32020.
PhosphoSitePlusiP32020.

Proteomic databases

MaxQBiP32020.
PaxDbiP32020.
PeptideAtlasiP32020.
PRIDEiP32020.
TopDownProteomicsiP32020-1. [P32020-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030340; ENSMUSP00000030340; ENSMUSG00000028603. [P32020-1]
ENSMUST00000106701; ENSMUSP00000102312; ENSMUSG00000028603. [P32020-2]
GeneIDi20280.
KEGGimmu:20280.
UCSCiuc008uas.2. mouse. [P32020-1]

Organism-specific databases

CTDi6342.
MGIiMGI:98254. Scp2.

Phylogenomic databases

eggNOGiKOG1406. Eukaryota.
KOG4170. Eukaryota.
ENOG410XPRW. LUCA.
GeneTreeiENSGT00530000062928.
HOGENOMiHOG000221741.
HOVERGENiHBG006506.
InParanoidiP32020.
KOiK08764.
OMAiHMEVMIN.
OrthoDBiEOG091G026S.
PhylomeDBiP32020.
TreeFamiTF300574.

Enzyme and pathway databases

BRENDAi2.3.1.176. 3474.
ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-2046106. alpha-linolenic acid (ALA) metabolism.
R-MMU-389887. Beta-oxidation of pristanoyl-CoA.

Miscellaneous databases

PROiP32020.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028603.
CleanExiMM_SCP2.
ExpressionAtlasiP32020. baseline and differential.
GenevisibleiP32020. MM.

Family and domain databases

Gene3Di3.30.1050.10. 1 hit.
3.40.47.10. 4 hits.
InterProiIPR003033. SCP2_sterol-bd_dom.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02036. SCP2. 1 hit.
PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
SSF55718. SSF55718. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNLTP_MOUSE
AccessioniPrimary (citable) accession number: P32020
Secondary accession number(s): A2APS2, A2APS3, Q9DBM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 16, 2003
Last modified: November 2, 2016
This is version 162 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.