Reviewed, UniProtKB/Swiss-Prot P32019 (I5P2_HUMAN)

Names and origin

Protein names

Recommended name:
    Type II inositol-1,4,5-trisphosphate 5-phosphatase
    EC=3.1.3.36
Alternative name(s):
    Phosphoinositide 5-phosphatase
      Short name=5PTase
    75 kDa inositol polyphosphate-5-phosphatase

Gene names

Name:

INPP5B

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	993 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes the calcium-mobilizing second messenger Ins(1,4,5)P3, this is a signal-terminating reaction.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Tissue specificity	Platelets.
Sequence similarities	Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family. Contains 1 Rho-GAP domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	inositol-polyphosphate 5-phosphatase activity Ref.1 Ref.5 Non-traceable author statement. Source: UniProtKB phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P32019-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: P32019-2)

The sequence of this isoform differs from the canonical sequence as follows:
     178-257: Missing.

Isoform 3 (identifier: P32019-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.

Isoform 4 (identifier: P32019-4)

The sequence of this isoform differs from the canonical sequence as follows:
     810-828: TLMPVWTGDDGSQLDSPME → LAYLAAYCFETQLVTKSLI
     829-993: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 320	320			PRO_0000015639
	Chain	321 – 993	673	Type II inositol-1,4,5-trisphosphate 5-phosphatase		PRO_0000015640
Regions
	Domain	821 – 993	173	Rho-GAP
Natural variations
	Alternative sequence	1 – 244	244	Missing in isoform 3.		VSP_012821
	Alternative sequence	178 – 257	80	Missing in isoform 2.		VSP_012820
	Alternative sequence	810 – 828	19	TLMPV…DSPME → LAYLAAYCFETQLVTKSLI in isoform 4.		VSP_013902
	Alternative sequence	829 – 993	165	Missing in isoform 4.		VSP_013903
	Natural variant	46	1	G → S: dbSNP rs56993041.		VAR_061270
	Natural variant	745	1	M → T: dbSNP rs11488569. Ref.1 Ref.3 Ref.5		VAR_028002
Experimental info
	Sequence conflict	587 – 606	20	GSDDW…WCDRI → RALTTGIPVRSAVLLPGVIG F AA sequence Ref.5
	Sequence conflict	911	1	G → P AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 15, 2009. Version 4. Checksum: ABD3581CC6CD29D6	FASTA	993	112,852
	10 20 30 40 50 60 MDQSVAIQET LAEGEYCVIA VQGVLCEGDS RQSRLLGLVR YRLEHGGQEH ALFLYTHRRM 70 80 90 100 110 120 AITGDDVSLD QIVPVSRDFT LEEVSPDGEL YILGSDVTVQ LDTAELSLVF QLPFGSQTRM 130 140 150 160 170 180 FLHEVARACP GFDSATRDPE FLWLSRYRCA ELELEMPTPR GCNSALVTWP GYATIGGGRY 190 200 210 220 230 240 PSRKKRWGLE EARPQGAGSV LFWGGAMEKT GFRLMERAHG GGFVWGRSAR DGRRDEELEE 250 260 270 280 290 300 AGREMSAAAG SRERNTAGGS NFDGLRPNGK GVPMDQSSRG QDKPESLQPR QNKSKSEITD 310 320 330 340 350 360 MVRSSTITVS DKAHILSMQK FGLRDTIVKS HLLQKEEDYT YIQNFRFFAG TYNVNGQSPK 370 380 390 400 410 420 ECLRLWLSNG IQAPDVYCVG FQELDLSKEA FFFHDTPKEE EWFKAVSEGL HPDAKYAKVK 430 440 450 460 470 480 LIRLVGIMLL LYVKQEHAAY ISEVEAETVG TGIMGRMGNK GGVAIRFQFH NTSICVVNSH 490 500 510 520 530 540 LAAHIEEYER RNQDYKDICS RMQFCQPDPS LPPLTISNHD VILWLGDLNY RIEELDVEKV 550 560 570 580 590 600 KKLIEEKDFQ MLYAYDQLKI QVAAKTVFEG FTEGELTFQP TYKYDTGSDD WDTSEKCRAP 610 620 630 640 650 660 AWCDRILWKG KNITQLSYQS HMALKTSDHK PVSSVFDIGV RVVNDELYRK TLEEIVRSLD 670 680 690 700 710 720 KMENANIPSV SLSKREFCFQ NVKYMQLKVE SFTIHNGQVP CHFEFINKPD EESYCKQWLN 730 740 750 760 770 780 ANPSRGFLLP DSDVEIDLEL FVNKMTATKL NSGEDKIEDI LVLHLDRGKD YFLSVSGNYL 790 800 810 820 830 840 PSCFGSPIHT LCYMREPILD LPLETISELT LMPVWTGDDG SQLDSPMEIP KELWMMVDYL 850 860 870 880 890 900 YRNAVQQEDL FQQPGLRSEF EHIRDCLDTG MIDNLSASNH SVAEALLLFL ESLPEPVICY 910 920 930 940 950 960 STYHNCLECS GNYTASKQVI STLPIFHKNV FHYLMAFLRE LLKNSAKNHL DENILASIFG 970 980 990 SLLLRNPAGH QKLDMTEKKK AQEFIHQFLC NPL
	Isoform 2. Checksum: 4882C26F6E4DC9C6	FASTA	913	103,987
	10 20 30 40 50 60 MDQSVAIQET LAEGEYCVIA VQGVLCEGDS RQSRLLGLVR YRLEHGGQEH ALFLYTHRRM 70 80 90 100 110 120 AITGDDVSLD QIVPVSRDFT LEEVSPDGEL YILGSDVTVQ LDTAELSLVF QLPFGSQTRM 130 140 150 160 170 180 FLHEVARACP GFDSATRDPE FLWLSRYRCA ELELEMPTPR GCNSALVTWP GYATIGGGGS 190 200 210 220 230 240 NFDGLRPNGK GVPMDQSSRG QDKPESLQPR QNKSKSEITD MVRSSTITVS DKAHILSMQK 250 260 270 280 290 300 FGLRDTIVKS HLLQKEEDYT YIQNFRFFAG TYNVNGQSPK ECLRLWLSNG IQAPDVYCVG 310 320 330 340 350 360 FQELDLSKEA FFFHDTPKEE EWFKAVSEGL HPDAKYAKVK LIRLVGIMLL LYVKQEHAAY 370 380 390 400 410 420 ISEVEAETVG TGIMGRMGNK GGVAIRFQFH NTSICVVNSH LAAHIEEYER RNQDYKDICS 430 440 450 460 470 480 RMQFCQPDPS LPPLTISNHD VILWLGDLNY RIEELDVEKV KKLIEEKDFQ MLYAYDQLKI 490 500 510 520 530 540 QVAAKTVFEG FTEGELTFQP TYKYDTGSDD WDTSEKCRAP AWCDRILWKG KNITQLSYQS 550 560 570 580 590 600 HMALKTSDHK PVSSVFDIGV RVVNDELYRK TLEEIVRSLD KMENANIPSV SLSKREFCFQ 610 620 630 640 650 660 NVKYMQLKVE SFTIHNGQVP CHFEFINKPD EESYCKQWLN ANPSRGFLLP DSDVEIDLEL 670 680 690 700 710 720 FVNKMTATKL NSGEDKIEDI LVLHLDRGKD YFLSVSGNYL PSCFGSPIHT LCYMREPILD 730 740 750 760 770 780 LPLETISELT LMPVWTGDDG SQLDSPMEIP KELWMMVDYL YRNAVQQEDL FQQPGLRSEF 790 800 810 820 830 840 EHIRDCLDTG MIDNLSASNH SVAEALLLFL ESLPEPVICY STYHNCLECS GNYTASKQVI 850 860 870 880 890 900 STLPIFHKNV FHYLMAFLRE LLKNSAKNHL DENILASIFG SLLLRNPAGH QKLDMTEKKK 910 AQEFIHQFLC NPL
	Isoform 3. Checksum: 50921FAACF7E1E39	FASTA	749	85,620
	10 20 30 40 50 60 MSAAAGSRER NTAGGSNFDG LRPNGKGVPM DQSSRGQDKP ESLQPRQNKS KSEITDMVRS 70 80 90 100 110 120 STITVSDKAH ILSMQKFGLR DTIVKSHLLQ KEEDYTYIQN FRFFAGTYNV NGQSPKECLR 130 140 150 160 170 180 LWLSNGIQAP DVYCVGFQEL DLSKEAFFFH DTPKEEEWFK AVSEGLHPDA KYAKVKLIRL 190 200 210 220 230 240 VGIMLLLYVK QEHAAYISEV EAETVGTGIM GRMGNKGGVA IRFQFHNTSI CVVNSHLAAH 250 260 270 280 290 300 IEEYERRNQD YKDICSRMQF CQPDPSLPPL TISNHDVILW LGDLNYRIEE LDVEKVKKLI 310 320 330 340 350 360 EEKDFQMLYA YDQLKIQVAA KTVFEGFTEG ELTFQPTYKY DTGSDDWDTS EKCRAPAWCD 370 380 390 400 410 420 RILWKGKNIT QLSYQSHMAL KTSDHKPVSS VFDIGVRVVN DELYRKTLEE IVRSLDKMEN 430 440 450 460 470 480 ANIPSVSLSK REFCFQNVKY MQLKVESFTI HNGQVPCHFE FINKPDEESY CKQWLNANPS 490 500 510 520 530 540 RGFLLPDSDV EIDLELFVNK MTATKLNSGE DKIEDILVLH LDRGKDYFLS VSGNYLPSCF 550 560 570 580 590 600 GSPIHTLCYM REPILDLPLE TISELTLMPV WTGDDGSQLD SPMEIPKELW MMVDYLYRNA 610 620 630 640 650 660 VQQEDLFQQP GLRSEFEHIR DCLDTGMIDN LSASNHSVAE ALLLFLESLP EPVICYSTYH 670 680 690 700 710 720 NCLECSGNYT ASKQVISTLP IFHKNVFHYL MAFLRELLKN SAKNHLDENI LASIFGSLLL 730 740 RNPAGHQKLD MTEKKKAQEF IHQFLCNPL
	Isoform 4. Checksum: 9CD5C12DCF509A50	FASTA	828	93,924
	10 20 30 40 50 60 MDQSVAIQET LAEGEYCVIA VQGVLCEGDS RQSRLLGLVR YRLEHGGQEH ALFLYTHRRM 70 80 90 100 110 120 AITGDDVSLD QIVPVSRDFT LEEVSPDGEL YILGSDVTVQ LDTAELSLVF QLPFGSQTRM 130 140 150 160 170 180 FLHEVARACP GFDSATRDPE FLWLSRYRCA ELELEMPTPR GCNSALVTWP GYATIGGGRY 190 200 210 220 230 240 PSRKKRWGLE EARPQGAGSV LFWGGAMEKT GFRLMERAHG GGFVWGRSAR DGRRDEELEE 250 260 270 280 290 300 AGREMSAAAG SRERNTAGGS NFDGLRPNGK GVPMDQSSRG QDKPESLQPR QNKSKSEITD 310 320 330 340 350 360 MVRSSTITVS DKAHILSMQK FGLRDTIVKS HLLQKEEDYT YIQNFRFFAG TYNVNGQSPK 370 380 390 400 410 420 ECLRLWLSNG IQAPDVYCVG FQELDLSKEA FFFHDTPKEE EWFKAVSEGL HPDAKYAKVK 430 440 450 460 470 480 LIRLVGIMLL LYVKQEHAAY ISEVEAETVG TGIMGRMGNK GGVAIRFQFH NTSICVVNSH 490 500 510 520 530 540 LAAHIEEYER RNQDYKDICS RMQFCQPDPS LPPLTISNHD VILWLGDLNY RIEELDVEKV 550 560 570 580 590 600 KKLIEEKDFQ MLYAYDQLKI QVAAKTVFEG FTEGELTFQP TYKYDTGSDD WDTSEKCRAP 610 620 630 640 650 660 AWCDRILWKG KNITQLSYQS HMALKTSDHK PVSSVFDIGV RVVNDELYRK TLEEIVRSLD 670 680 690 700 710 720 KMENANIPSV SLSKREFCFQ NVKYMQLKVE SFTIHNGQVP CHFEFINKPD EESYCKQWLN 730 740 750 760 770 780 ANPSRGFLLP DSDVEIDLEL FVNKMTATKL NSGEDKIEDI LVLHLDRGKD YFLSVSGNYL 790 800 810 820 PSCFGSPIHT LCYMREPILD LPLETISELL AYLAAYCFET QLVTKSLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Properties of type II inositol polyphosphate 5-phosphatase." Jefferson A.B., Majerus P.W. J. Biol. Chem. 270:9370-9377(1995) [PubMed: 7721860] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT THR-745.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), VARIANT THR-745. Tissue: Lymph.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-178. Tissue: Stomach.
[5]	"Cloning and expression of human 75-kDa inositol polyphosphate-5-phosphatase." Ross T.S., Jefferson A.B., Mitchell C.A., Majerus P.W. J. Biol. Chem. 266:20283-20289(1991) [PubMed: 1718960] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 284-954, PROTEIN SEQUENCE OF 321-339, VARIANT THR-745. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74161 mRNA. Translation: AAA79207.1. Different initiation. AL603790, AL929472 Genomic DNA. Translation: CAH69926.1. AL603790, AL929472 Genomic DNA. Translation: CAH69928.1. AL603790, AL929472 Genomic DNA. Translation: CAH69929.1. AL929472, AL603790 Genomic DNA. Translation: CAH70076.1. AL929472, AL603790 Genomic DNA. Translation: CAH70079.1. AL929472, AL603790 Genomic DNA. Translation: CAH70080.1. BC042529 mRNA. Translation: AAH42529.2. BC058932 mRNA. Translation: AAH58932.1. AL833055 mRNA. Translation: CAH56301.1.
IPI	IPI00244111. IPI00478376. IPI00553072. IPI00604769.
RefSeq	NP_005531.2.
UniGene	Hs.449942
3D structure databases
SMR	P32019. Positions 325-664, 673-991.
ModBase	Search...
Protein-protein interaction databases
STRING	P32019.
PTM databases
PhosphoSite	P32019.
Genome annotation databases
Ensembl	ENST00000373023; ENSP00000362114; ENSG00000204084; Homo sapiens. [Genome view] ENST00000373026; ENSP00000362117; ENSG00000204084; Homo sapiens. [Genome view]
GeneID	3633.
KEGG	hsa:3633.
UCSC	uc001ccf.1. human. uc001ccg.1. human. uc009vvk.1. human.
Organism-specific databases
CTD	3633.
GeneCards	GC01M038162.
H-InvDB	HIX0000445.
HGNC	HGNC:6077. INPP5B.
MIM	147264. gene.
PharmGKB	PA29885.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04448.
HOVERGEN	P32019.
InParanoid	P32019.
Enzyme and pathway databases
BioCyc	MetaCyc:ENSG00000134678-MONOMER.
BRENDA	3.1.3.36. 247.
Reactome	REACT_11044. Signaling by Rho GTPases.
Gene expression databases
ArrayExpress	P32019.
Bgee	P32019.
CleanEx	HS_INPP5B.
Genevestigator	P32019.
GermOnline	ENSG00000204084. Homo sapiens.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. SM00324. RhoGAP. 1 hit. [Graphical view]
PROSITE	PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	14219.
SOURCE	Search...

Entry information

Entry name

I5P2_HUMAN

Accession

Primary (citable) accession number: P32019
Secondary accession number(s): Q5VSG9 , Q5VSH0, Q5VSH1, Q658Q5, Q6P6D4, Q6PD53, Q86YE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	December 15, 2009
Last modified:	February 9, 2010
This is version 92 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.