Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Daunorubicin/doxorubicin resistance ATP-binding protein DrrA

Gene

drrA

Organism
Streptomyces peucetius
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex DrrAB involved in daunorubicin and doxorubicin resistance. Responsible for energy coupling to the transport system. Binds ATP or GTP.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 488ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.1.105.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Daunorubicin/doxorubicin resistance ATP-binding protein DrrA (EC:3.6.3.-)
Gene namesi
Name:drrA
OrganismiStreptomyces peucetius
Taxonomic identifieri1950 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

  • cytoplasmic side of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Daunorubicin/doxorubicin resistance ATP-binding protein DrrAPRO_0000092319Add
BLAST

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (DrrA) and two transmembrane proteins (DrrB).1 Publication

Structurei

3D structure databases

ProteinModelPortaliP32010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 239231ABC transporterPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005894. DrrA.
IPR025302. DUF4162.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF13732. DUF4162. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01188. drrA. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTQPTRAIE TSGLVKVYNG TRAVDGLDLN VPAGLVYGIL GPNGAGKSTT
60 70 80 90 100
IRMLATLLRP DGGTARVFGH DVTSEPDTVR RRISVTGQYA SVDEGLTGTE
110 120 130 140 150
NLVMMGRLQG YSWARARERA AELIDGFGLG DARDRLLKTY SGGMRRRLDI
160 170 180 190 200
AASIVVTPDL LFLDEPTTGL DPRSRNQVWD IVRALVDAGT TVLLTTQYLD
210 220 230 240 250
EADQLADRIA VIDHGRVIAE GTTGELKSSL GSNVLRLRLH DAQSRAEAER
260 270 280 290 300
LLSAELGVTI HRDSDPTALS ARIDDPRQGM RALAELSRTH LEVRSFSLGQ
310 320 330
SSLDEVFLAL TGHPADDRST EEAAEEEKVA
Length:330
Mass (Da):35,700
Last modified:July 1, 1993 - v1
Checksum:i582D66C90D54E6B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73758 Genomic DNA. Translation: AAA74717.1.
PIRiS27707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73758 Genomic DNA. Translation: AAA74717.1.
PIRiS27707.

3D structure databases

ProteinModelPortaliP32010.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.A.1.105.1. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005894. DrrA.
IPR025302. DUF4162.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF13732. DUF4162. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01188. drrA. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin."
    Guilfoile P.G., Hutchinson C.R.
    Proc. Natl. Acad. Sci. U.S.A. 88:8553-8557(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN RESISTANCE.
    Strain: ATCC 29050 / DSM 40754 / JCM 9920 / NBRC 100596 / NCIMB 10972.
  2. "Expression and characterization of DrrA and DrrB proteins of Streptomyces peucetius in Escherichia coli: DrrA is an ATP binding protein."
    Kaur P.
    J. Bacteriol. 179:569-575(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ATP-BINDING, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDRRA_STRPE
AccessioniPrimary (citable) accession number: P32010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.