Reviewed, UniProtKB/Swiss-Prot P32001 (RPOD_BUCAP)

Names and origin

Protein names

Recommended name:
    RNA polymerase sigma factor rpoD
Alternative name(s):
    Sigma-70

Gene names

Name:	rpoD
Ordered Locus Names:	BUsg_052

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
Sequence similarities	Belongs to the sigma-70 factor family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation Inferred from electronic annotation. Source: InterPro
Molecular function	sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	RNA polymerase sigma factor rpoD		PRO_0000093878
Regions
	DNA binding	577 – 596	20	H-T-H motif By similarity
	Motif	407 – 420	14	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	P32001-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 95DC5864D463AD67	FASTA	617	71,470
	10 20 30 40 50 60 MEHNPKSQLK LLVTHGKEQG YLTYAEVNDH LPEEIIDSEQ IDDIIQMIND MGIQVVEEAP 70 80 90 100 110 120 DADDLILNEI NTDTDEDAVE AATQVLSSVE SELGRTTDPV RMYMREMGTV ELLTREGEID 130 140 150 160 170 180 IAKRIEEGIN QVQSSVSEYP EAITYLLEQY DRIKTGQIRL SDIITGFVDP NAEEIFSHST 190 200 210 220 230 240 SAIHIGSEIL DNITNDSNEN EDENEDENED EDENSIDPEL ANEKFRELRK QYTNTSNTIK 250 260 270 280 290 300 NKNRNHQDSL LEIYNLSEIF KQFRLVPKQF DHLVNNMRNM MNRVRKQERK IMKLCIEECK 310 320 330 340 350 360 IPKKIFLKIF SGKETDQNWF KKEQSSNQSW SKKLEEIKEK VFSSMKKLKQ IEKETGLTIE 370 380 390 400 410 420 QVKDINKRMS IGEAKAKRAK KEMVEANLRL VISIAKKYTN RGLQFLDLIQ EGNIGLMKAV 430 440 450 460 470 480 DKFEYRRGYK FSTYATWWIR QAITRSIADQ ARTIRIPVHM IETINKLNRI SRQMLQEIGR 490 500 510 520 530 540 EPTPEELSEK MLIPEDKIRK VLKIAKEPIS METPIGDDDD SHLGDFIEDT TLELPLDSAT 550 560 570 580 590 600 SESLRSATHD VLSGLTAREA KVLRMRFGID MNTDHTLEEV GKQFDVTRER IRQIEAKALR 610 KLRHPSRSEV LRSFLDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a DNA fragment from Buchnera aphidicola (an endosymbiont of aphids) containing genes homologous to dnaG, rpoD, cysE, and secB." Lai C.-Y., Baumann P. Gene 119:113-118(1992) [PubMed: 1398077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M90644 Genomic DNA. Translation: AAA73234.1. AE013218 Genomic DNA. Translation: AAM67623.1.
PIR	RNJV7A. JC1291.
RefSeq	NP_660412.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005868.
GenomeReviews	Gene locus BUsg_052 in contig AE013218_GR.
KEGG	bas:BUsg052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P32001.
OMA	HPSRSET
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG052-MON.
Family and domain databases
InterPro	IPR014284. RNA_pol_sigma-70. IPR000943. RNA_pol_sigma70. IPR009042. RNA_pol_sigma70_r1_2. IPR007627. RNA_pol_sigma70_r2. IPR007624. RNA_pol_sigma70_r3. IPR007630. RNA_pol_sigma70_r4. IPR007631. RNA_pol_sigma_70_non-ess. IPR007127. RNA_pol_sigma_70_r1.1. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012760. RNA_pol_sigma_RpoD_C. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 2 hits.
Pfam	PF04546. Sigma70_ner. 1 hit. PF03979. Sigma70_r1_1. 1 hit. PF00140. Sigma70_r1_2. 1 hit. PF04542. Sigma70_r2. 1 hit. PF04539. Sigma70_r3. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
TIGRFAMs	TIGR02393. RpoD_Cterm. 1 hit. TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOD_BUCAP

Accession

Primary (citable) accession number: P32001

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)