P32000 (PRIM_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase
EC=2.7.7.-

Gene names

Name:	dnaG
Ordered Locus Names:	BUsg_053

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.
Cofactor	Binds 1 zinc ion per monomer By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA primase family. Contains 1 Toprim domain.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA primase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 579	579	DNA primase		PRO_0000180482
Regions
	Domain	258 – 340	83	Toprim
	Zinc finger	40 – 64	25	CHC2-type By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32000 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 51D937DCF56DB75A	FASTA	579	68,270
	10 20 30 40 50 60 MSGKIPKYFI NELLFRTNIV ELIETRIKLK KNGKNYQTHC PFHNDKTPSF TVSYEKQFYY 70 80 90 100 110 120 CFGCSAHGNA IDFLINYDNL NFVESIEELA TIHSIEIPFD KKGTIEKKIY LKKQKLYFLM 130 140 150 160 170 180 DKMSKLYQKN IIFTNSANKY LTERGINKNM IDFFCIGFSS NKWDLFSKQF NIKKDFEKDL 190 200 210 220 230 240 LHYKIISTNK NGYIYDTFQG RIVFPIHDQH GRIISFGGRS ITNNILPKYL NSHENDIFHK 250 260 270 280 290 300 GKQIYGLYQV KKKHSKPKYL LVVEGYIDVI MLTQYNIEYV VSSLGTSITK EHIQILFRHT 310 320 330 340 350 360 NTVIYCYDGD DAGRNASWRT LKIALPYISD KKNIKFIILP NNEDPDTIIK KEGQKKFQLR 370 380 390 400 410 420 IDNALTMSKF FFKNILKNVD LSSDDDKFHL SVRALPLINC ISSDTIRIYL RQILARTIGI 430 440 450 460 470 480 VDDYQFEKFL YEKERKKKSL QFQIKHTPMR TLIGLLIQNP NLARLVPSTK KFENEKIQGL 490 500 510 520 530 540 PIFLEILKTC SDNPNFNTGQ LLEFYRYSKI INILKILSKW DHMIIKEKIQ NMFLDLLKNT 550 560 570 YSKILEKRQE KLIAKERIQG LTIDEKKEIW SINKKLSKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
[2]	"Sequence analysis of a DNA fragment from Buchnera aphidicola (an endosymbiont of aphids) containing genes homologous to dnaG, rpoD, cysE, and secB." Lai C.-Y., Baumann P. Gene 119:113-118(1992) [PubMed: 1398077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 261-579.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67624.1. M90644 Genomic DNA. Translation: AAA73233.1.
PIR	PC1137.
RefSeq	NP_660413.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P32000.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000540; EBBUCP00000000540; EBBUCG00000000540.
GeneID	1005869.
GenomeReviews	Gene locus BUsg_053 in contig AE013218_GR.
KEGG	bas:BUsg053.
PATRIC	21246871. VBIBucAph100086_0053.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007723.
HOGENOM	HBG573408.
OMA	LFEARNA.
ProtClustDB	CLSK315071.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG053-MONOMER.
Family and domain databases
InterPro	IPR016136. DNA_helicase_N/primase_C. IPR013264. DNA_primase_core_N. IPR019475. DNA_primase_DnaB-bd. IPR006295. DNA_primase_DnaG. IPR013173. DNA_primase_DnaG_DnaB-bd. IPR006171. Toprim_domain. IPR002694. Znf_CHC2. [Graphical view]
Gene3D	G3DSA:1.10.860.10. DnaB_N. 1 hit. G3DSA:3.90.980.10. Toprim_N. 1 hit. G3DSA:3.90.580.10. Znf_CHC2. 1 hit.
KO	K02316.
Pfam	PF10410. DnaB_bind. 1 hit. PF08278. DnaG_DnaB_bind. 1 hit. PF01751. Toprim. 1 hit. PF08275. Toprim_N. 1 hit. PF01807. zf-CHC2. 1 hit. [Graphical view]
SMART	SM00766. DnaG_DnaB_bind. 1 hit. SM00493. TOPRIM. 1 hit. SM00400. ZnF_CHCC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01391. DnaG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRIM_BUCAP

Accession

Primary (citable) accession number: P32000

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	August 30, 2002
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program