P31997 (CEAM8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carcinoembryonic antigen-related cell adhesion molecule 8

Alternative name(s):
CD67 antigen
Carcinoembryonic antigen CGM6
Non-specific cross-reacting antigen NCA-95
CD_antigen=CD66b

Gene names

Name:	CEACAM8
Synonyms:	CGM6

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Expressed in leukocytes of chronic myeloid Leukemia patients and bone marrow.
Sequence similarities	Belongs to the immunoglobulin superfamily. CEA family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	immune response Traceable author statement. Source: ProtInc
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular space Traceable author statement. Source: ProtInc integral to plasma membrane Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 34

34

Chain

35 – 320

286

Carcinoembryonic antigen-related cell adhesion molecule 8

PRO_0000014572

Propeptide

321 – 349

29

Removed in mature form

PRO_0000014573

Regions

Domain

35 – 142

108

Ig-like V-type

Domain

145 – 232

88

Ig-like C2-type 1

Domain

237 – 319

83

Ig-like C2-type 2

Amino acid modifications

Lipidation

320

1

GPI-anchor amidated aspartate

Glycosylation

104

1

N-linked (GlcNAc...) Potential

Glycosylation

111

1

N-linked (GlcNAc...) Potential

Glycosylation

115

1

N-linked (GlcNAc...) Potential

Glycosylation

152

1

N-linked (GlcNAc...) Potential

Glycosylation

173

1

N-linked (GlcNAc...) Potential

Glycosylation

197

1

N-linked (GlcNAc...) Potential

Glycosylation

224

1

N-linked (GlcNAc...) Potential

Glycosylation

256

1

N-linked (GlcNAc...) Potential

Glycosylation

274

1

N-linked (GlcNAc...) Potential

Glycosylation

288

1

N-linked (GlcNAc...) Ref.7

Glycosylation

309

1

N-linked (GlcNAc...) Potential

Disulfide bond

167 ↔ 215

Probable

Disulfide bond

259 ↔ 299

Probable

Natural variations

Natural variant

17

1

G → V.
Corresponds to variant rs28367882 [ dbSNP | Ensembl ].

VAR_049849

Natural variant

45

1

A → V.
Corresponds to variant rs35221575 [ dbSNP | Ensembl ].

VAR_049850

Natural variant

114

1

R → K.
Corresponds to variant rs1041997 [ dbSNP | Ensembl ].

VAR_011721

Natural variant

322

1

L → V. Ref.2
Corresponds to variant rs45476198 [ dbSNP | Ensembl ].

VAR_049851

Natural variant

340

1

I → M.
Corresponds to variant rs8103051 [ dbSNP | Ensembl ].

VAR_049852

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

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.

.

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.

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349

Helix Strand Turn

Details...

Beta strand

37 – 46

10

Beta strand

51 – 57

7

Beta strand

60 – 62

3

Beta strand

66 – 68

3

Helix

75 – 77

3

Beta strand

78 – 80

3

Turn

84 – 87

4

Beta strand

99 – 101

3

Beta strand

107 – 109

3

Turn

114 – 116

3

Turn

127 – 129

3

Beta strand

139 – 141

3

Sequences

	Sequence		Length	Mass (Da)
	P31997 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: AACF74DA1AC839D8	FASTA	349	38,154
	10 20 30 40 50 60 MGPISAPSCR WRIPWQGLLL TASLFTFWNP PTTAQLTIEA VPSNAAEGKE VLLLVHNLPQ 70 80 90 100 110 120 DPRGYNWYKG ETVDANRRII GYVISNQQIT PGPAYSNRET IYPNASLLMR NVTRNDTGSY 130 140 150 160 170 180 TLQVIKLNLM SEEVTGQFSV HPETPKPSIS SNNSNPVEDK DAVAFTCEPE TQNTTYLWWV 190 200 210 220 230 240 NGQSLPVSPR LQLSNGNRTL TLLSVTRNDV GPYECEIQNP ASANFSDPVT LNVLYGPDAP 250 260 270 280 290 300 TISPSDTYYH AGVNLNLSCH AASNPPSQYS WSVNGTFQQY TQKLFIPNIT TKNSGSYACH 310 320 330 340 TTNSATGRNR TTVRMITVSD ALVQGSSPGL SARATVSIMI GVLARVALI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a carcinoembryonic antigen gene family member expressed in leukocytes of chronic myeloid leukemia patients and bone marrow." Berling B., Kolbinger F., Grunert F., Thompson J.A., Brombacher F., Buchegger F., Vkleist S., Zimmermann W. Cancer Res. 50:6534-6539(1990) [PubMed: 2208113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[2]	"Characterization of a cDNA clone encoding a new species of the nonspecific cross-reacting antigen (NCA), a member of the CEA gene family." Arakawa F., Kuroki M., Misumi Y., Oikawa S., Nakazato H., Matsuoka Y. Biochem. Biophys. Res. Commun. 166:1063-1071(1990) [PubMed: 2306228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-322.
[3]	"Identification of three new genes and estimation of the size of the carcinoembryonic antigen family." Khan W.N., Fraengsmyr L., Teglund S., Israelsson A., Bremer K., Hammarstroem S. Genomics 14:384-390(1992) [PubMed: 1427854] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	"Mice transgenic for the human CGM6 gene express its product, the granulocyte marker CD66b, exclusively in granulocytes." Eades-Perner A., Thompson J., van der Putten H., Zimmermann W. Blood 91:663-672(1998) [PubMed: 9427723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
[7]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed: 16740002] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-288, MASS SPECTROMETRY. Tissue: Saliva.
[8]	"Solution structure of the first Ig-like domain of human carcinoembryonic antigen related cell adhesion molecule 8." RIKEN structural genomics initiative (RSGI) Submitted (MAY-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 33-154.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X52378 mRNA. Translation: CAA36604.1.
M33326 mRNA. Translation: AAA59914.1.
D90064 mRNA. Translation: BAA14108.1.
AC004558 Genomic DNA. Translation: AAC13659.1.
BC026263 mRNA. Translation: AAH26263.1.
Z95119 Genomic DNA. Translation: CAB08298.1.

IPI

IPI00013972.

PIR

A34815. S13524.

RefSeq

NP_001807.2. NM_001816.3.

UniGene

Hs.41.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DKS	NMR	-	A	33-149	[»]

ProteinModelPortal

P31997.

SMR

P31997. Positions 33-322.

ModBase

Search...

Protein-protein interaction databases

IntAct

P31997. 2 interactions.

STRING

P31997.

Polymorphism databases

DMDM

6166048.

Proteomic databases

PeptideAtlas

P31997.

PRIDE

P31997.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000244336; ENSP00000244336; ENSG00000124469.

GeneID

1088.

KEGG

hsa:1088.

UCSC

uc002oud.1. human.

Organism-specific databases

CTD

1088.

GeneCards

GC19M043084.

H-InvDB

HIX0018406.

HGNC

HGNC:1820. CEACAM8.

MIM

114890. gene.

neXtProt

NX_P31997.

PharmGKB

PA26364.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG12390.

HOGENOM

HBG506003.

HOVERGEN

HBG007922.

InParanoid

P31997.

OMA

SGSYACH.

OrthoDB

EOG41ZFBV.

PhylomeDB

P31997.

Gene expression databases

ArrayExpress

P31997.

Bgee

P31997.

CleanEx

HS_CEACAM8.

Genevestigator

P31997.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 3 hits.

KO

K06499.

Pfam

PF07679. I-set. 1 hit.
PF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00409. IG. 3 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

4526.

SOURCE

Search...

Entry information

Entry name

CEAM8_HUMAN

Accession

Primary (citable) accession number: P31997
Secondary accession number(s): O60399, Q16574

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 15, 1999
Last modified:	January 25, 2012
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.