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Protein

Macrosialin

Gene

Cd68

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

GO - Biological processi

  • cellular response to organic substance Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Macrosialin
Alternative name(s):
CD_antigen: CD68
Gene namesi
Name:Cd68
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88342. Cd68.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 291271ExtracellularSequence analysisAdd
BLAST
Transmembranei292 – 31625HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini317 – 32610CytoplasmicPROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 326306MacrosialinPRO_0000017103Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence analysis
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence analysis
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi139 ↔ 177PROSITE-ProRule annotation
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis
Glycosylationi218 – 2181N-linked (GlcNAc...)Sequence analysis
Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence analysis
Disulfide bondi249 ↔ 286PROSITE-ProRule annotation
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N- and O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP31996.
PaxDbiP31996.
PeptideAtlasiP31996.
PRIDEiP31996.

PTM databases

iPTMnetiP31996.
PhosphoSiteiP31996.
SwissPalmiP31996.

Expressioni

Tissue specificityi

Expressed in tissue macrophages and to a lesser extent in dendritic cells.

Gene expression databases

BgeeiENSMUSG00000018774.
CleanExiMM_CD68.
GenevisibleiP31996. MM.

Interactioni

Protein-protein interaction databases

IntActiP31996. 1 interaction.
MINTiMINT-4104943.
STRINGi10090.ENSMUSP00000104294.

Structurei

3D structure databases

ProteinModelPortaliP31996.
SMRiP31996. Positions 288-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati44 – 4961-1
Repeati50 – 64152-1Add
BLAST
Repeati65 – 7281-2
Repeati73 – 88162-2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 10989Mucin-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi110 – 12112Pro-rich (hinge)Add
BLAST

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000070034.
HOVERGENiHBG005287.
InParanoidiP31996.
KOiK06501.
PhylomeDBiP31996.
TreeFamiTF316339.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P31996-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLPVCLILL GPLIAQGTEE DCPHKKAVTL LPSFTMTPTA TESTASPTTS
60 70 80 90 100
HRPTTTSHGN VTVHTSSGPT TVTHNPATTT SHGNATISHA TVSPTTNGTA
110 120 130 140 150
TSPRSSTVGP HPGPPPPSPS PRSKGALGNY TWANGSQPCV QLQAQIQIRI
160 170 180 190 200
LYPIQGGRKA WGISVLNPNK TKVQGGCDGT HPHLSLSFPY GQLTFGFKQD
210 220 230 240 250
LHQSPSTVYL DYMAVEYNVS FPQAAQWTFM AQNSSLRELQ APLGQSFCCG
260 270 280 290 300
NASIVLSPAV HLDLLSLRLQ AAQLPDKGHF GPCFSCNRDQ SLLLPLIIGL
310 320
VLLGLLTLVL IAFCITRRRQ STYQPL
Length:326
Mass (Da):34,818
Last modified:July 1, 1993 - v1
Checksum:iAB7203A9A7EA47BA
GO
Isoform Short (identifier: P31996-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-326: Missing.

Show »
Length:318
Mass (Da):33,844
Checksum:iCB1AF96E96BD1DE9
GO

Sequence cautioni

The sequence BAA23738 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171P → T in AAC15685 (PubMed:9806844).Curated
Sequence conflicti129 – 1291N → K in BAB21975 (PubMed:16141072).Curated
Sequence conflicti137 – 1371Q → H in AAC15685 (PubMed:9806844).Curated
Sequence conflicti205 – 2051P → T in BAB21975 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei319 – 3268Missing in isoform Short. 1 PublicationVSP_003043

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68273 mRNA. Translation: CAA48334.1.
AF022651 Genomic DNA. Translation: AAC40151.1.
AF045554 Genomic DNA. Translation: AAC15685.1.
AF039399 Genomic DNA. Translation: AAC40056.1.
AB009287 Genomic DNA. Translation: BAA23738.1. Sequence problems.
AK002264 mRNA. Translation: BAB21975.1.
AK170443 mRNA. Translation: BAE41801.1.
AL603707 Genomic DNA. Translation: CAI51942.1.
BC021637 mRNA. Translation: AAH21637.1.
DQ167574 mRNA. Translation: ABA02181.1.
CCDSiCCDS70216.1. [P31996-1]
PIRiA46676.
RefSeqiNP_001277987.1. NM_001291058.1. [P31996-1]
UniGeneiMm.15819.

Genome annotation databases

EnsembliENSMUST00000018918; ENSMUSP00000018918; ENSMUSG00000018774. [P31996-1]
GeneIDi12514.
KEGGimmu:12514.
UCSCiuc007jqy.2. mouse. [P31996-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68273 mRNA. Translation: CAA48334.1.
AF022651 Genomic DNA. Translation: AAC40151.1.
AF045554 Genomic DNA. Translation: AAC15685.1.
AF039399 Genomic DNA. Translation: AAC40056.1.
AB009287 Genomic DNA. Translation: BAA23738.1. Sequence problems.
AK002264 mRNA. Translation: BAB21975.1.
AK170443 mRNA. Translation: BAE41801.1.
AL603707 Genomic DNA. Translation: CAI51942.1.
BC021637 mRNA. Translation: AAH21637.1.
DQ167574 mRNA. Translation: ABA02181.1.
CCDSiCCDS70216.1. [P31996-1]
PIRiA46676.
RefSeqiNP_001277987.1. NM_001291058.1. [P31996-1]
UniGeneiMm.15819.

3D structure databases

ProteinModelPortaliP31996.
SMRiP31996. Positions 288-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP31996. 1 interaction.
MINTiMINT-4104943.
STRINGi10090.ENSMUSP00000104294.

PTM databases

iPTMnetiP31996.
PhosphoSiteiP31996.
SwissPalmiP31996.

Proteomic databases

MaxQBiP31996.
PaxDbiP31996.
PeptideAtlasiP31996.
PRIDEiP31996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018918; ENSMUSP00000018918; ENSMUSG00000018774. [P31996-1]
GeneIDi12514.
KEGGimmu:12514.
UCSCiuc007jqy.2. mouse. [P31996-1]

Organism-specific databases

CTDi968.
MGIiMGI:88342. Cd68.

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000070034.
HOVERGENiHBG005287.
InParanoidiP31996.
KOiK06501.
PhylomeDBiP31996.
TreeFamiTF316339.

Miscellaneous databases

PROiP31996.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018774.
CleanExiMM_CD68.
GenevisibleiP31996. MM.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD68_MOUSE
AccessioniPrimary (citable) accession number: P31996
Secondary accession number(s): O54688
, O70321, Q3S4A9, Q5F2A8, Q9DD15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

CD68 is a commonly used marker for macrophages. However, a number of studies in human have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.