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Protein

Macrosialin

Gene

Cd68

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

GO - Biological processi

  • cellular response to organic substance Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Macrosialin
Alternative name(s):
CD_antigen: CD68
Gene namesi
Name:Cd68
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88342. Cd68.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 291ExtracellularSequence analysisAdd BLAST271
Transmembranei292 – 316HelicalPROSITE-ProRule annotationAdd BLAST25
Topological domaini317 – 326CytoplasmicPROSITE-ProRule annotation10

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001710321 – 326MacrosialinAdd BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...)Sequence analysis1
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi134N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi139 ↔ 177PROSITE-ProRule annotation
Glycosylationi169N-linked (GlcNAc...)Sequence analysis1
Glycosylationi218N-linked (GlcNAc...)Sequence analysis1
Glycosylationi233N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi249 ↔ 286PROSITE-ProRule annotation
Glycosylationi251N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N- and O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP31996.
PaxDbiP31996.
PeptideAtlasiP31996.
PRIDEiP31996.

PTM databases

iPTMnetiP31996.
PhosphoSitePlusiP31996.
SwissPalmiP31996.

Expressioni

Tissue specificityi

Expressed in tissue macrophages and to a lesser extent in dendritic cells.

Gene expression databases

BgeeiENSMUSG00000018774.
CleanExiMM_CD68.
ExpressionAtlasiP31996. baseline and differential.
GenevisibleiP31996. MM.

Interactioni

Protein-protein interaction databases

IntActiP31996. 1 interactor.
MINTiMINT-4104943.
STRINGi10090.ENSMUSP00000104294.

Structurei

3D structure databases

ProteinModelPortaliP31996.
SMRiP31996.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati44 – 491-16
Repeati50 – 642-1Add BLAST15
Repeati65 – 721-28
Repeati73 – 882-2Add BLAST16

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 109Mucin-likeAdd BLAST89

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi110 – 121Pro-rich (hinge)Add BLAST12

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000070034.
HOVERGENiHBG005287.
InParanoidiP31996.
KOiK06501.
PhylomeDBiP31996.
TreeFamiTF316339.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P31996-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLPVCLILL GPLIAQGTEE DCPHKKAVTL LPSFTMTPTA TESTASPTTS
60 70 80 90 100
HRPTTTSHGN VTVHTSSGPT TVTHNPATTT SHGNATISHA TVSPTTNGTA
110 120 130 140 150
TSPRSSTVGP HPGPPPPSPS PRSKGALGNY TWANGSQPCV QLQAQIQIRI
160 170 180 190 200
LYPIQGGRKA WGISVLNPNK TKVQGGCDGT HPHLSLSFPY GQLTFGFKQD
210 220 230 240 250
LHQSPSTVYL DYMAVEYNVS FPQAAQWTFM AQNSSLRELQ APLGQSFCCG
260 270 280 290 300
NASIVLSPAV HLDLLSLRLQ AAQLPDKGHF GPCFSCNRDQ SLLLPLIIGL
310 320
VLLGLLTLVL IAFCITRRRQ STYQPL
Length:326
Mass (Da):34,818
Last modified:July 1, 1993 - v1
Checksum:iAB7203A9A7EA47BA
GO
Isoform Short (identifier: P31996-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-326: Missing.

Show »
Length:318
Mass (Da):33,844
Checksum:iCB1AF96E96BD1DE9
GO

Sequence cautioni

The sequence BAA23738 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117P → T in AAC15685 (PubMed:9806844).Curated1
Sequence conflicti129N → K in BAB21975 (PubMed:16141072).Curated1
Sequence conflicti137Q → H in AAC15685 (PubMed:9806844).Curated1
Sequence conflicti205P → T in BAB21975 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003043319 – 326Missing in isoform Short. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68273 mRNA. Translation: CAA48334.1.
AF022651 Genomic DNA. Translation: AAC40151.1.
AF045554 Genomic DNA. Translation: AAC15685.1.
AF039399 Genomic DNA. Translation: AAC40056.1.
AB009287 Genomic DNA. Translation: BAA23738.1. Sequence problems.
AK002264 mRNA. Translation: BAB21975.1.
AK170443 mRNA. Translation: BAE41801.1.
AL603707 Genomic DNA. Translation: CAI51942.1.
BC021637 mRNA. Translation: AAH21637.1.
DQ167574 mRNA. Translation: ABA02181.1.
CCDSiCCDS70216.1. [P31996-1]
PIRiA46676.
RefSeqiNP_001277987.1. NM_001291058.1. [P31996-1]
UniGeneiMm.15819.

Genome annotation databases

EnsembliENSMUST00000018918; ENSMUSP00000018918; ENSMUSG00000018774. [P31996-1]
GeneIDi12514.
KEGGimmu:12514.
UCSCiuc007jqy.2. mouse. [P31996-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68273 mRNA. Translation: CAA48334.1.
AF022651 Genomic DNA. Translation: AAC40151.1.
AF045554 Genomic DNA. Translation: AAC15685.1.
AF039399 Genomic DNA. Translation: AAC40056.1.
AB009287 Genomic DNA. Translation: BAA23738.1. Sequence problems.
AK002264 mRNA. Translation: BAB21975.1.
AK170443 mRNA. Translation: BAE41801.1.
AL603707 Genomic DNA. Translation: CAI51942.1.
BC021637 mRNA. Translation: AAH21637.1.
DQ167574 mRNA. Translation: ABA02181.1.
CCDSiCCDS70216.1. [P31996-1]
PIRiA46676.
RefSeqiNP_001277987.1. NM_001291058.1. [P31996-1]
UniGeneiMm.15819.

3D structure databases

ProteinModelPortaliP31996.
SMRiP31996.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP31996. 1 interactor.
MINTiMINT-4104943.
STRINGi10090.ENSMUSP00000104294.

PTM databases

iPTMnetiP31996.
PhosphoSitePlusiP31996.
SwissPalmiP31996.

Proteomic databases

MaxQBiP31996.
PaxDbiP31996.
PeptideAtlasiP31996.
PRIDEiP31996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018918; ENSMUSP00000018918; ENSMUSG00000018774. [P31996-1]
GeneIDi12514.
KEGGimmu:12514.
UCSCiuc007jqy.2. mouse. [P31996-1]

Organism-specific databases

CTDi968.
MGIiMGI:88342. Cd68.

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000070034.
HOVERGENiHBG005287.
InParanoidiP31996.
KOiK06501.
PhylomeDBiP31996.
TreeFamiTF316339.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP31996.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018774.
CleanExiMM_CD68.
ExpressionAtlasiP31996. baseline and differential.
GenevisibleiP31996. MM.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD68_MOUSE
AccessioniPrimary (citable) accession number: P31996
Secondary accession number(s): O54688
, O70321, Q3S4A9, Q5F2A8, Q9DD15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

CD68 is a commonly used marker for macrophages. However, a number of studies in human have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.