Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tautomerase PptA

Gene

pptA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can use enol isomers of phenylpyruvate, 2-hydroxy-2,4-pentadienoate and (p-hydroxyphenyl)pyruvate as substrates.1 Publication

Enzyme regulationi

Inhibited by (E)-2-fluoro-p-hydroxycinnamate.1 Publication

Kineticsi

  1. KM=205 µM for enol isomers of phenylpyruvate1 Publication
  2. KM=220 µM for 2-hydroxy-2,4-pentadienoate1 Publication
  3. KM=180 µM for (p-hydroxyphenyl)pyruvate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei2Proton acceptor; via imino nitrogen1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11761-MONOMER.
    ECOL316407:JW1456-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tautomerase PptA (EC:5.3.2.-)
    Gene namesi
    Name:pptA
    Synonyms:ydcE
    Ordered Locus Names:b1461, JW1456
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11761. pptA.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB03793. Benzoic Acid.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved
    ChainiPRO_00002095262 – 77Tautomerase PptAAdd BLAST76

    Proteomic databases

    PaxDbiP31992.
    PRIDEiP31992.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi4262891. 27 interactors.
    IntActiP31992. 14 interactors.
    STRINGi511145.b1461.

    Structurei

    Secondary structure

    177
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi3 – 9Combined sources7
    Helixi15 – 33Combined sources19
    Helixi37 – 39Combined sources3
    Beta strandi41 – 46Combined sources6
    Helixi49 – 51Combined sources3
    Helixi52 – 58Combined sources7
    Turni59 – 66Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1GYJX-ray2.10A/B2-77[»]
    1GYXX-ray1.35A/B2-77[»]
    1GYYX-ray1.35A/B2-77[»]
    ProteinModelPortaliP31992.
    SMRiP31992.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP31992.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105KZK. Bacteria.
    COG1942. LUCA.
    HOGENOMiHOG000077848.
    KOiK01821.
    OMAiIKCFPRD.

    Family and domain databases

    HAMAPiMF_00718. Tautomerase_PptA. 1 hit.
    InterProiIPR004370. 4-oxalocrotonate_tautomerase.
    IPR014347. Tautomerase/MIF_sf.
    IPR017284. Tautomerase_PptA.
    [Graphical view]
    PfamiPF01361. Tautomerase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037799. Tautomer_YdcE_prd. 1 hit.
    SUPFAMiSSF55331. SSF55331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31992-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPHIDIKCFP RELDEQQKAA LAADITDVII RHLNSKDSSI SIALQQIQPE
    60 70
    SWQAIWDAEI APQMEALIKK PGYSMNA
    Length:77
    Mass (Da):8,673
    Last modified:January 23, 2007 - v3
    Checksum:iD32FF4023FE556CA
    GO

    Mass spectrometryi

    Molecular mass is 8540 Da from positions 2 - 77. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X60998 Genomic DNA. Translation: CAA43312.1.
    U00096 Genomic DNA. Translation: AAC74543.1.
    AP009048 Genomic DNA. Translation: BAA15092.1.
    PIRiH64898.
    RefSeqiNP_415978.1. NC_000913.3.
    WP_001120143.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74543; AAC74543; b1461.
    BAA15092; BAA15092; BAA15092.
    GeneIDi945731.
    KEGGiecj:JW1456.
    eco:b1461.
    PATRICi32118214. VBIEscCol129921_1527.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X60998 Genomic DNA. Translation: CAA43312.1.
    U00096 Genomic DNA. Translation: AAC74543.1.
    AP009048 Genomic DNA. Translation: BAA15092.1.
    PIRiH64898.
    RefSeqiNP_415978.1. NC_000913.3.
    WP_001120143.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1GYJX-ray2.10A/B2-77[»]
    1GYXX-ray1.35A/B2-77[»]
    1GYYX-ray1.35A/B2-77[»]
    ProteinModelPortaliP31992.
    SMRiP31992.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262891. 27 interactors.
    IntActiP31992. 14 interactors.
    STRINGi511145.b1461.

    Chemistry databases

    DrugBankiDB03793. Benzoic Acid.

    Proteomic databases

    PaxDbiP31992.
    PRIDEiP31992.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC74543; AAC74543; b1461.
    BAA15092; BAA15092; BAA15092.
    GeneIDi945731.
    KEGGiecj:JW1456.
    eco:b1461.
    PATRICi32118214. VBIEscCol129921_1527.

    Organism-specific databases

    EchoBASEiEB1711.
    EcoGeneiEG11761. pptA.

    Phylogenomic databases

    eggNOGiENOG4105KZK. Bacteria.
    COG1942. LUCA.
    HOGENOMiHOG000077848.
    KOiK01821.
    OMAiIKCFPRD.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11761-MONOMER.
    ECOL316407:JW1456-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP31992.
    PROiP31992.

    Family and domain databases

    HAMAPiMF_00718. Tautomerase_PptA. 1 hit.
    InterProiIPR004370. 4-oxalocrotonate_tautomerase.
    IPR014347. Tautomerase/MIF_sf.
    IPR017284. Tautomerase_PptA.
    [Graphical view]
    PfamiPF01361. Tautomerase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037799. Tautomer_YdcE_prd. 1 hit.
    SUPFAMiSSF55331. SSF55331. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPPTA_ECOLI
    AccessioniPrimary (citable) accession number: P31992
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: January 23, 2007
    Last modified: November 2, 2016
    This is version 127 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.