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Protein

NADH-quinone oxidoreductase subunit F

Gene

nuoF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • FMNCuratedNote: Binds 1 FMN.Curated
  • [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi351 – 3511Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi354 – 3541Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi357 – 3571Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi398 – 3981Iron-sulfur (4Fe-4S)Sequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 7010NADBy similarity
Nucleotide bindingi174 – 22148FMNBy similarityAdd
BLAST

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • FMN binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • NAD binding Source: EcoCyc
  • NADH dehydrogenase (ubiquinone) activity Source: InterPro
  • quinone binding Source: UniProtKB-KW

GO - Biological processi

  • aerobic respiration Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NUOF-MONOMER.
ECOL316407:JW2279-MONOMER.
MetaCyc:NUOF-MONOMER.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit F (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F
NUO6
Gene namesi
Name:nuoF
Ordered Locus Names:b2284, JW2279
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11774. nuoF.

Subcellular locationi

GO - Cellular componenti

  • NADH dehydrogenase complex Source: EcoliWiki
  • plasma membrane Source: EcoliWiki
  • plasma membrane respiratory chain complex I Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445NADH-quinone oxidoreductase subunit FPRO_0000118568Add
BLAST

Keywords - PTMi

Quinone

Proteomic databases

EPDiP31979.
PaxDbiP31979.
PRIDEiP31979.

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.

Protein-protein interaction databases

BioGridi4260513. 24 interactions.
DIPiDIP-10382N.
IntActiP31979. 7 interactions.
STRINGi511145.b2284.

Structurei

3D structure databases

ProteinModelPortaliP31979.
SMRiP31979. Positions 21-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Phylogenomic databases

eggNOGiENOG4107QIZ. Bacteria.
COG1894. LUCA.
HOGENOMiHOG000251534.
InParanoidiP31979.
KOiK00335.
OMAiNTFCAHA.
PhylomeDBiP31979.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIIRTPET HPLTWRLRDD KQPVWLDEYR SKNGYEGARK ALTGLSPDEI
60 70 80 90 100
VNQVKDAGLK GRGGAGFSTG LKWSLMPKDE SMNIRYLLCN ADEMEPGTYK
110 120 130 140 150
DRLLMEQLPH LLVEGMLISA FALKAYRGYI FLRGEYIEAA VNLRRAIAEA
160 170 180 190 200
TEAGLLGKNI MGTGFDFELF VHTGAGRYIC GEETALINSL EGRRANPRSK
210 220 230 240 250
PPFPATSGAW GKPTCVNNVE TLCNVPAILA NGVEWYQNIS KSKDAGTKLM
260 270 280 290 300
GFSGRVKNPG LWELPFGTTA REILEDYAGG MRDGLKFKAW QPGGAGTDFL
310 320 330 340 350
TEAHLDLPME FESIGKAGSR LGTALAMAVD HEINMVSLVR NLEEFFARES
360 370 380 390 400
CGWCTPCRDG LPWSVKILRA LERGEGQPGD IETLEQLCRF LGPGKTFCAH
410 420 430 440
APGAVEPLQS AIKYFREEFE AGIKQPFSNT HLINGIQPNL LKERW
Length:445
Mass (Da):49,292
Last modified:November 1, 1997 - v3
Checksum:i7F39A7DBE50C2075
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 119NIIRTPETH → KHYPYSRND in AAA53584 (PubMed:8366049).Curated
Sequence conflicti26 – 261L → R in CAA48365 (PubMed:7690854).Curated
Sequence conflicti38 – 381A → R in AAA53584 (PubMed:8366049).Curated
Sequence conflicti41 – 411A → R in CAA48365 (PubMed:7690854).Curated
Sequence conflicti51 – 511V → I in AAA53584 (PubMed:8366049).Curated
Sequence conflicti370 – 3701A → R (PubMed:7690854).Curated
Sequence conflicti370 – 3701A → R (PubMed:8157582).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48365.1.
L25055 Unassigned DNA. Translation: AAA03537.1.
U00096 Genomic DNA. Translation: AAC75344.1.
AP009048 Genomic DNA. Translation: BAA16113.1.
L19569 Genomic DNA. Translation: AAA53584.1.
PIRiB65000.
RefSeqiNP_416787.1. NC_000913.3.
WP_000789500.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75344; AAC75344; b2284.
BAA16113; BAA16113; BAA16113.
GeneIDi946753.
KEGGiecj:JW2279.
eco:b2284.
PATRICi32119935. VBIEscCol129921_2377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA. Translation: CAA48365.1.
L25055 Unassigned DNA. Translation: AAA03537.1.
U00096 Genomic DNA. Translation: AAC75344.1.
AP009048 Genomic DNA. Translation: BAA16113.1.
L19569 Genomic DNA. Translation: AAA53584.1.
PIRiB65000.
RefSeqiNP_416787.1. NC_000913.3.
WP_000789500.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31979.
SMRiP31979. Positions 21-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260513. 24 interactions.
DIPiDIP-10382N.
IntActiP31979. 7 interactions.
STRINGi511145.b2284.

Protein family/group databases

TCDBi3.D.1.1.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

EPDiP31979.
PaxDbiP31979.
PRIDEiP31979.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75344; AAC75344; b2284.
BAA16113; BAA16113; BAA16113.
GeneIDi946753.
KEGGiecj:JW2279.
eco:b2284.
PATRICi32119935. VBIEscCol129921_2377.

Organism-specific databases

EchoBASEiEB1723.
EcoGeneiEG11774. nuoF.

Phylogenomic databases

eggNOGiENOG4107QIZ. Bacteria.
COG1894. LUCA.
HOGENOMiHOG000251534.
InParanoidiP31979.
KOiK00335.
OMAiNTFCAHA.
PhylomeDBiP31979.

Enzyme and pathway databases

BioCyciEcoCyc:NUOF-MONOMER.
ECOL316407:JW2279-MONOMER.
MetaCyc:NUOF-MONOMER.

Miscellaneous databases

PROiP31979.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUOF_ECOLI
AccessioniPrimary (citable) accession number: P31979
Secondary accession number(s): P78239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.