P31977 (EZRI_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ezrin

Alternative name(s):
Cytovillin
Villin-2
p81

Gene names

Name:	Ezr
Synonyms:	Vil2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis By similarity.
Enzyme regulation	A head-to-tail association, of the N-terminal and C-terminal halves results in a closed conformation (inactive form) which is incapable of actin or membrane-binding By similarity.
Subunit structure	Interacts with MCC, MPP5, PLEKHG6, SCYL3/PACE1, SLC9A3R1, SCYL3/PACE1 and TMEM8B. Interacts (when phosphorylated) with FES/FPS By similarity. Found in a complex with EZR, PODXL and SLC9A3R2. Interacts with PODXL and SLC9A3R2. Ref.6
Subcellular location	Apical cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell projection By similarity. Cell projection › microvillus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell projection › ruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm › cell cortex By similarity. Cytoplasm › cytoskeleton By similarity. Note: Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Microvillar peripheral membrane protein (cytoplasmic side) By similarity. Localizes to cell extensions and peripheral processes of astrocytes. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells. Ref.6 Ref.7
Tissue specificity	Glomerular epithelium cell (podocyte). Expressed in cerebrum, cerebellum and hippocampus (at protein level). Expressed in the small intestine, lung, kidney and ovaries. Ref.4 Ref.6 Ref.7
Developmental stage	Levels increase in the fetal gut epithelium between day 15 and day 20 of gestation and during the first week after birth. Ref.4
Post-translational modification	Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding By similarity.
Sequence similarities	Contains 1 FERM domain.

Ontologies

Keywords
Biological process	Cell shape
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane
Domain	Coiled coil
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	actin filament bundle assembly Inferred from sequence or structural similarity. Source: UniProtKB epithelial cell differentiation Inferred from expression pattern Ref.4. Source: RGD establishment or maintenance of apical/basal cell polarity Inferred from electronic annotation. Source: Compara filopodium assembly Inferred from mutant phenotype PubMed 21753079. Source: RGD leukocyte cell-cell adhesion Inferred from electronic annotation. Source: Compara membrane to membrane docking Inferred from electronic annotation. Source: Compara regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Schwann cell microvillus Inferred from direct assay PubMed 17045809. Source: RGD T-tubule Inferred from direct assay PubMed 19028724. Source: RGD actin filament Inferred from sequence or structural similarity. Source: UniProtKB apical plasma membrane Inferred from direct assay Ref.6. Source: UniProtKB astrocyte projection Inferred from direct assay PubMed 21753079. Source: RGD basolateral plasma membrane Inferred from direct assay PubMed 11726633. Source: UniProtKB cell body Inferred from direct assay PubMed 11461930. Source: RGD cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cell tip Inferred from direct assay PubMed 17061246. Source: RGD cytosol Traceable author statement. Source: Reactome extrinsic to membrane Inferred from sequence or structural similarity. Source: UniProtKB filopodium Inferred from direct assay PubMed 21753079. Source: RGD focal adhesion Inferred from electronic annotation. Source: Compara microspike Inferred from direct assay PubMed 21753079. Source: RGD microtubule basal body Inferred from electronic annotation. Source: Compara microvillus membrane Inferred from direct assay PubMed 11726633. Source: UniProtKB nucleolus Inferred from electronic annotation. Source: Compara ruffle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell uropod Inferred from electronic annotation. Source: Compara
Molecular_function	actin filament binding Inferred from sequence or structural similarity. Source: UniProtKB cell adhesion molecule binding Inferred from sequence or structural similarity. Source: BHF-UCL structural molecule activity Traceable author statement Ref.4. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 586	585	Ezrin		PRO_0000219411
Regions
	Domain	2 – 295	294	FERM
	Region	244 – 586	343	Interaction with SCYL3 By similarity
	Coiled coil	300 – 466	167	Potential
Amino acid modifications
	Modified residue	60	1	N6-acetyllysine By similarity
	Modified residue	146	1	Phosphotyrosine; by PDGFR By similarity
	Modified residue	535	1	Phosphoserine By similarity
	Modified residue	567	1	Phosphothreonine; by ROCK2 and PKC/PRKCI By similarity
Experimental info
	Sequence conflict	455	1	D → E in AAL47844. Ref.3
	Sequence conflict	531	1	L → P in CAA48004. Ref.4
	Sequence conflict	532	1	L → Q in AAR91694. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31977 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3A5287052E74CCC9	FASTA	586	69,391
	10 20 30 40 50 60 MPKPINVRVT TMDAELEFAI QPNTTGKQLF DQVVKTIGLR EVWYFGLQYV DNKGFPTWLK 70 80 90 100 110 120 LDKKVSAQEV RKENPVQFKF RAKFYPEDVA DELIQDITQK LFFLQVKEGI LSDEIYCPPE 130 140 150 160 170 180 TAVLLGSYAV QAKFGDYNKE MHKSGYLSSE RLIPQRVMDQ HKLSRDQWED RIQVWHAEHR 190 200 210 220 230 240 GMLKDSAMLE YLKIAQDLEM YGINYFEIKN KKGTDLWLGV DALGLNIYEK DDKLTPKIGF 250 260 270 280 290 300 PWSEIRNISF NDKKFVIKPI DKKAPDFVFY APRLRINKRI LQLCMGNHEL YMRRRKPDTI 310 320 330 340 350 360 EVQQMKAQAR EEKHQKQLER QQLETEKKRR ETVEREKEQM LREKEELMLR LQDFEQKTKR 370 380 390 400 410 420 AEKELSEQIE KALQLEEERR RAQEEAERLE ADRMAALRAK EELERQAQDQ IKSQEQLAAE 430 440 450 460 470 480 LAEYTAKIAL LEEARRRKED EVEEWQHRAK EAQDDLVKTK EELHLVMTAP PPPPPPVYEP 490 500 510 520 530 540 VNYHVQEGLQ DEGAEPMGYS AELSSEGILD DRNEEKRITE AEKNERVQRQ LLTLSNELSQ 550 560 570 580 ARDENKRTHN DIIHNENMRQ GRDKYKTLRQ IRQGNTKQRI DEFEAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rattus norvegicus ezrin." Harita Y., Koike H., Han G., Miyauchi N., Karasawa T., Suzuki K., Shimizu F., Kawachi H. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	Gunn-Moore F.J., Tait S., Brophy P.J. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-455. Strain: Sprague-Dawley.
[4]	"Transcriptional regulation of the ezrin gene during rat intestinal development and epithelial differentiation." Barila D., Murgia C., Nobili F., Perozzi G. Biochim. Biophys. Acta 1263:133-140(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 427-586, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Wistar. Tissue: Intestine.
[5]	Lubec G., Chen W.-Q., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 28-35; 101-107; 263-273; 372-379; 428-435 AND 530-542, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Hippocampus.
[6]	"Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton." Takeda T., McQuistan T., Orlando R.A., Farquhar M.G. J. Clin. Invest. 108:289-301(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PODXL AND SLC9A3R2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Characterization of the NF2 protein merlin and the ERM protein ezrin in human, rat, and mouse central nervous system." Groenholm M., Teesalu T., Tyynelaa J., Piltti K., Boehling T., Wartiovaara K., Vaheri A., Carpen O. Mol. Cell. Neurosci. 28:683-693(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY428869 mRNA. Translation: AAR91694.1. BC081958 mRNA. Translation: AAH81958.1. AF450298 mRNA. Translation: AAL47844.1. X67788 mRNA. Translation: CAA48004.1.
IPI	IPI00470254.
PIR	S58759.
RefSeq	NP_062230.1. NM_019357.1.
UniGene	Rn.773.
3D structure databases
ProteinModelPortal	P31977.
SMR	P31977. Positions 1-385, 500-586.
ModBase	Search...
Protein-protein interaction databases
IntAct	P31977. 2 interactions.
PTM databases
PhosphoSite	P31977.
2D gel databases
World-2DPAGE	0004:P31977.
Proteomic databases
PaxDb	P31977.
PRIDE	P31977.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000046746; ENSRNOP00000046593; ENSRNOG00000018524.
GeneID	54319.
KEGG	rno:54319.
Organism-specific databases
CTD	7430.
RGD	621161. Ezr.
Phylogenomic databases
eggNOG	NOG236035.
GeneTree	ENSGT00650000092953.
HOGENOM	HOG000007113.
HOVERGEN	HBG002185.
InParanoid	P31977.
KO	K08007.
OrthoDB	EOG4C5CJJ.
Enzyme and pathway databases
Reactome	REACT_96538. Developmental Biology.
Gene expression databases
ArrayExpress	P31977.
Genevestigator	P31977.
GermOnline	ENSRNOG00000018524. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.80.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR019749. Band_41_domain. IPR019750. Band_41_fam. IPR011174. ERM. IPR011259. ERM_C_dom. IPR000798. Ez/rad/moesin_like. IPR014352. FERM/acyl-CoA-bd_prot_3-hlx. IPR019748. FERM_central. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR018979. FERM_N. IPR018980. FERM_PH-like_C. IPR008954. Moesin. IPR011993. PH_like_dom. [Graphical view]
Pfam	PF00769. ERM. 1 hit. PF09380. FERM_C. 1 hit. PF00373. FERM_M. 1 hit. PF09379. FERM_N. 1 hit. [Graphical view]
PIRSF	PIRSF002305. ERM. 1 hit.
PRINTS	PR00935. BAND41. PR00661. ERMFAMILY.
SMART	SM00295. B41. 1 hit. [Graphical view]
SUPFAM	SSF47031. FERM_3-hlx. 1 hit. SSF48678. Moesin. 1 hit.
PROSITE	PS00660. FERM_1. 1 hit. PS00661. FERM_2. 1 hit. PS50057. FERM_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	611002.

Entry information

Entry name

EZRI_RAT

Accession

Primary (citable) accession number: P31977
Secondary accession number(s): Q5WQV4, Q66H97, Q8VHK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program