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P31974 (MTA1_CELCE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase AluI

Short name=M.AluI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase AluI
Gene names
Name:aluIM
OrganismCellulosimicrobium cellulans (Arthrobacter luteus)
Taxonomic identifier1710 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaePromicromonosporaceaeCellulosimicrobium

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence AGCT, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the AluI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 521521Modification methylase AluI
PRO_0000087858

Regions

Domain8 – 491484SAM-dependent MTase C5-type

Sites

Active site841 By similarity

Sequences

Sequence LengthMass (Da)Tools
P31974 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: ACBBB2C4E5326BFB

FASTA52158,648
        10         20         30         40         50         60 
MSKANAKYSF VDLFAGIGGF HAALAATGGV CEYAVEIDRE AAAVYERNWN KPALGDITDD 

        70         80         90        100        110        120 
ANDEGVTLRG YDGPIDVLTG GFPCQPFSKS GAQHGMAETR GTLFWNIARI IEEREPTVLI 

       130        140        150        160        170        180 
LENVRNLVGP RHRHEWLTII ETLRFFGYEV SGAPAIFSPH LLPAWMGGTP QVRERVFITA 

       190        200        210        220        230        240 
TLVPERMRDE RIPRTETGEI DAEAIGPKPV ATMNDRFPIK KGGTELFHPG DRKSGWNLLT 

       250        260        270        280        290        300 
SGIIREGDPE PSNVDLRLTE TETLWIDAWD DLESTIRRAT GRPLEGFPYW ADSWTDFREL 

       310        320        330        340        350        360 
SRLVVIRGFQ APEREVVGDR KRYVARTDMP EGFVPASVTR PAIDETLPAW KQSHLRRNYD 

       370        380        390        400        410        420 
FFERHFAEVV AWAYRWGVYT DLFPASRRKL EWQAQDAPRL WDTVMHFRPS GIRAKRPTYL 

       430        440        450        460        470        480 
PALVAITQTS IVGPLERRLS PRETARLQGL PEWFDFGEQR AAATYKQMGN GVNVGVVRHI 

       490        500        510        520 
LREHVRRDRA LLKLTPAGQR IINAVLADEP DATVGALGAA E 

« Hide

References

[1]"The M.AluI DNA-(cytosine C5)-methyltransferase has an unusually large, partially dispensable, variable region."
Zhang B., Tao T., Wilson G.G., Blumenthal R.M.
Nucleic Acids Res. 21:905-911(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 21606 / DSM 20424 / 73-14.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11841 Genomic DNA. Translation: CAA77866.1.
PIRS35614.

3D structure databases

ProteinModelPortalP31974.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTA1_CELCE
AccessionPrimary (citable) accession number: P31974
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries