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P31974

- MTA1_CELCE

UniProt

P31974 - MTA1_CELCE

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Protein

Modification methylase AluI

Gene

aluIM

Organism
Cellulosimicrobium cellulans (Arthrobacter luteus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence AGCT, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the AluI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei84 – 841PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase AluI (EC:2.1.1.37)
Short name:
M.AluI
Alternative name(s):
Cytosine-specific methyltransferase AluI
Gene namesi
Name:aluIM
OrganismiCellulosimicrobium cellulans (Arthrobacter luteus)
Taxonomic identifieri1710 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaePromicromonosporaceaeCellulosimicrobium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Modification methylase AluIPRO_0000087858Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP31974.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 491484SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31974-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKANAKYSF VDLFAGIGGF HAALAATGGV CEYAVEIDRE AAAVYERNWN
60 70 80 90 100
KPALGDITDD ANDEGVTLRG YDGPIDVLTG GFPCQPFSKS GAQHGMAETR
110 120 130 140 150
GTLFWNIARI IEEREPTVLI LENVRNLVGP RHRHEWLTII ETLRFFGYEV
160 170 180 190 200
SGAPAIFSPH LLPAWMGGTP QVRERVFITA TLVPERMRDE RIPRTETGEI
210 220 230 240 250
DAEAIGPKPV ATMNDRFPIK KGGTELFHPG DRKSGWNLLT SGIIREGDPE
260 270 280 290 300
PSNVDLRLTE TETLWIDAWD DLESTIRRAT GRPLEGFPYW ADSWTDFREL
310 320 330 340 350
SRLVVIRGFQ APEREVVGDR KRYVARTDMP EGFVPASVTR PAIDETLPAW
360 370 380 390 400
KQSHLRRNYD FFERHFAEVV AWAYRWGVYT DLFPASRRKL EWQAQDAPRL
410 420 430 440 450
WDTVMHFRPS GIRAKRPTYL PALVAITQTS IVGPLERRLS PRETARLQGL
460 470 480 490 500
PEWFDFGEQR AAATYKQMGN GVNVGVVRHI LREHVRRDRA LLKLTPAGQR
510 520
IINAVLADEP DATVGALGAA E
Length:521
Mass (Da):58,648
Last modified:July 1, 1993 - v1
Checksum:iACBBB2C4E5326BFB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11841 Genomic DNA. Translation: CAA77866.1.
PIRiS35614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11841 Genomic DNA. Translation: CAA77866.1 .
PIRi S35614.

3D structure databases

ProteinModelPortali P31974.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The M.AluI DNA-(cytosine C5)-methyltransferase has an unusually large, partially dispensable, variable region."
    Zhang B., Tao T., Wilson G.G., Blumenthal R.M.
    Nucleic Acids Res. 21:905-911(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 21606 / DSM 20424 / 73-14.

Entry informationi

Entry nameiMTA1_CELCE
AccessioniPrimary (citable) accession number: P31974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3