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P31974

- MTA1_CELCE

UniProt

P31974 - MTA1_CELCE

Protein

Modification methylase AluI

Gene

aluIM

Organism
Cellulosimicrobium cellulans (Arthrobacter luteus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence AGCT, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the AluI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei84 – 841PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase AluI (EC:2.1.1.37)
    Short name:
    M.AluI
    Alternative name(s):
    Cytosine-specific methyltransferase AluI
    Gene namesi
    Name:aluIM
    OrganismiCellulosimicrobium cellulans (Arthrobacter luteus)
    Taxonomic identifieri1710 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaePromicromonosporaceaeCellulosimicrobium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 521521Modification methylase AluIPRO_0000087858Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP31974.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 491484SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31974-1 [UniParc]FASTAAdd to Basket

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    MSKANAKYSF VDLFAGIGGF HAALAATGGV CEYAVEIDRE AAAVYERNWN    50
    KPALGDITDD ANDEGVTLRG YDGPIDVLTG GFPCQPFSKS GAQHGMAETR 100
    GTLFWNIARI IEEREPTVLI LENVRNLVGP RHRHEWLTII ETLRFFGYEV 150
    SGAPAIFSPH LLPAWMGGTP QVRERVFITA TLVPERMRDE RIPRTETGEI 200
    DAEAIGPKPV ATMNDRFPIK KGGTELFHPG DRKSGWNLLT SGIIREGDPE 250
    PSNVDLRLTE TETLWIDAWD DLESTIRRAT GRPLEGFPYW ADSWTDFREL 300
    SRLVVIRGFQ APEREVVGDR KRYVARTDMP EGFVPASVTR PAIDETLPAW 350
    KQSHLRRNYD FFERHFAEVV AWAYRWGVYT DLFPASRRKL EWQAQDAPRL 400
    WDTVMHFRPS GIRAKRPTYL PALVAITQTS IVGPLERRLS PRETARLQGL 450
    PEWFDFGEQR AAATYKQMGN GVNVGVVRHI LREHVRRDRA LLKLTPAGQR 500
    IINAVLADEP DATVGALGAA E 521
    Length:521
    Mass (Da):58,648
    Last modified:July 1, 1993 - v1
    Checksum:iACBBB2C4E5326BFB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11841 Genomic DNA. Translation: CAA77866.1.
    PIRiS35614.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11841 Genomic DNA. Translation: CAA77866.1 .
    PIRi S35614.

    3D structure databases

    ProteinModelPortali P31974.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The M.AluI DNA-(cytosine C5)-methyltransferase has an unusually large, partially dispensable, variable region."
      Zhang B., Tao T., Wilson G.G., Blumenthal R.M.
      Nucleic Acids Res. 21:905-911(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 21606 / DSM 20424 / 73-14.

    Entry informationi

    Entry nameiMTA1_CELCE
    AccessioniPrimary (citable) accession number: P31974
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3