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P31970

- SYE_SYNP2

UniProt

P31970 - SYE_SYNP2

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 4 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei252 – 2521ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSSP32049:GKF7-1395-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:SYNPCC7002_A1394
    OrganismiSynechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum)
    Taxonomic identifieri32049 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000001688: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 481481Glutamate--tRNA ligasePRO_0000119675Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi32049.SYNPCC7002_A1394.

    Structurei

    3D structure databases

    ProteinModelPortaliP31970.
    SMRiP31970. Positions 2-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi9 – 1911"HIGH" regionAdd
    BLAST
    Motifi249 – 2535"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiDIDMQIS.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31970-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVRVRIAPS PTGNLHIGTA RTAVFNWLFA HHHGGTFVLR VEDTDLERSK    50
    PEYTENIKTG LQWLGLHWDE GPFFQTQRLD QYKAAIQTLL DQGLAYRCYC 100
    TPAELEAMRE QQKANNQAPR YDNRHRNLTE AQRAEFEAQG RKPVIRFKID 150
    DAQQIVWQDL IRGTMTWKGS DLGGDMVIAR TPEGDESFGQ PLYNLAVVVD 200
    DIDMQISHVI RGEDHIANTA KQILLYEALG AAVPQFAHTP LILNQEGRKL 250
    SKRDGVTSID DFRQMGFLPQ AIANYMSLLG WTPTDSTQEI FTLEEAAKEF 300
    SLERVNKAGA KFDWDKLDWI NAQYLHQMPI PALTDLLIPY LQAAGYGDYL 350
    GDRPWLESLV ALVAPSLTRL ADVTQETRLL FGDSITLDEK ATAQLQTEGV 400
    KVILQEILQN IQASTNFTPD EAKALINQAT KAHGVKKGVV MKSMRAGLMG 450
    ELQGPDLMQS WVLLHQKGWD IERLNQAIAS I 481
    Length:481
    Mass (Da):54,135
    Last modified:June 10, 2008 - v4
    Checksum:i71E6FB7E30925E49
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000951 Genomic DNA. Translation: ACA99390.1.
    M99379 Genomic DNA. Translation: AAA27356.1.
    RefSeqiWP_012307013.1. NC_010475.1.
    YP_001734646.1. NC_010475.1.

    Genome annotation databases

    EnsemblBacteriaiACA99390; ACA99390; SYNPCC7002_A1394.
    GeneIDi6055293.
    KEGGisyp:SYNPCC7002_A1394.
    PATRICi23817378. VBISynSp37135_1638.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000951 Genomic DNA. Translation: ACA99390.1 .
    M99379 Genomic DNA. Translation: AAA27356.1 .
    RefSeqi WP_012307013.1. NC_010475.1.
    YP_001734646.1. NC_010475.1.

    3D structure databases

    ProteinModelPortali P31970.
    SMRi P31970. Positions 2-478.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 32049.SYNPCC7002_A1394.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA99390 ; ACA99390 ; SYNPCC7002_A1394 .
    GeneIDi 6055293.
    KEGGi syp:SYNPCC7002_A1394.
    PATRICi 23817378. VBISynSp37135_1638.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi DIDMQIS.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci SSP32049:GKF7-1395-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Synechococcus sp. PCC 7002."
      Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T., Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J., Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.
      Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27264 / PCC 7002 / PR-6.
    2. "Cloning and characterization of the psaE gene of the cyanobacterium Synechococcus sp. PCC 7002: characterization of a psaE mutant and overproduction of the protein in Escherichia coli."
      Zhao J., Snyder W., Muhlenhoff U., Rhiel E., Bryant D.A.
      Mol. Microbiol. 9:183-194(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-160.

    Entry informationi

    Entry nameiSYE_SYNP2
    AccessioniPrimary (citable) accession number: P31970
    Secondary accession number(s): B1XMA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 97 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3