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P31955 (AREG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amphiregulin

Short name=AR
Alternative name(s):
Schwannoma-derived growth factor
Short name=SDGF
Gene names
Name:Areg
Synonyms:Sdgf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Subunit structure

The immature precursor interacts with CNIH By similarity.

Subcellular location

Membrane; Single-pass membrane protein.

Induction

Androgen-dependent.

Sequence similarities

Belongs to the amphiregulin family.

Contains 1 EGF-like domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainEGF-like domain
Signal
Transmembrane
Transmembrane helix
   Molecular functionCytokine
Growth factor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdichotomous subdivision of terminal units involved in mammary gland duct morphogenesis

Inferred from mutant phenotype PubMed 17369357. Source: MGI

epidermal growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

epithelial cell proliferation involved in mammary gland duct elongation

Inferred from mutant phenotype PubMed 17369357. Source: MGI

glial cell proliferation

Inferred from electronic annotation. Source: Ensembl

mammary gland alveolus development

Inferred from genetic interaction PubMed 10331984. Source: MGI

mammary gland branching involved in thelarche

Inferred from mutant phenotype PubMed 10331984PubMed 17369357. Source: MGI

negative regulation of osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

neuron projection development

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of phosphorylation

Inferred from electronic annotation. Source: Ensembl

response to cAMP

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from direct assay PubMed 17369357. Source: MGI

response to glucocorticoid

Inferred from electronic annotation. Source: Ensembl

response to hydrogen peroxide

Inferred from electronic annotation. Source: Ensembl

response to peptide hormone

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 8588926. Source: MGI

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular

Inferred from direct assay PubMed 8588926. Source: MGI

nucleus

Inferred from direct assay PubMed 8588926. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Propeptide27 – 9973
PRO_0000007476
Chain100 – 248149Amphiregulin
PRO_0000007477

Regions

Transmembrane192 – 21524Helical; Potential
Domain135 – 17541EGF-like

Amino acid modifications

Glycosylation1061N-linked (GlcNAc...) Potential
Glycosylation2411N-linked (GlcNAc...) Potential
Disulfide bond139 ↔ 152 By similarity
Disulfide bond147 ↔ 163 By similarity
Disulfide bond165 ↔ 174 By similarity

Sequences

Sequence LengthMass (Da)Tools
P31955 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 98C61A1B0E75A64E

FASTA24827,549
        10         20         30         40         50         60 
MRTPLLPLAR SVLLLLVLGS GHYAAALELN DPSSGKGESL SGDHSAGGLE LSVGREVSTI 

        70         80         90        100        110        120 
SEMPSGSELS TGDYDYSEEY DNEPQISGYI IDDSVRVEQV IKPKKNKTEG EKSTEKPKRK 

       130        140        150        160        170        180 
KKGGKNGKGR RNKKKKNPCT AKFQNFCIHG ECRYIENLEV VTCNCHQDYF GERCGEKSMK 

       190        200        210        220        230        240 
THSEDDKDLS KIAVVAVTIF VSAIILAAIG IGIVITVHLW KRYFREYEGE TEERRRLRQE 


NGTVHAIA 

« Hide

References

« Hide 'large scale' references
[1]"Androgen-responsive expression and mitogenic activity of schwannoma-derived growth factor on an androgen-dependent Shionogi mouse mammary carcinoma cell line."
Sonoda H., Yamaguchi T., Watanabe S.
Biochem. Biophys. Res. Commun. 185:103-109(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Amphiregulin is an implantation-specific and progesterone-regulated gene in the mouse uterus."
Das S.K., Chakraborty I., Paria B.C., Wang X.N., Plowman G.D., Dey S.K.
Mol. Endocrinol. 9:691-705(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cecum.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D12648 mRNA. Translation: BAA02169.1.
L41352 Genomic DNA. Translation: AAB00472.1.
AK018590 mRNA. Translation: BAB31296.1.
BC009138 mRNA. Translation: AAH09138.1.
PIRJH0612.
RefSeqNP_033834.1. NM_009704.3.
UniGeneMm.8039.

3D structure databases

ProteinModelPortalP31955.
SMRP31955. Positions 135-177.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000031325.

PTM databases

PhosphoSiteP31955.

Proteomic databases

PRIDEP31955.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000031325; ENSMUSP00000031325; ENSMUSG00000029378.
GeneID11839.
KEGGmmu:11839.
UCSCuc008ybt.1. mouse.

Organism-specific databases

CTD374.
MGIMGI:88068. Areg.

Phylogenomic databases

eggNOGNOG41450.
HOGENOMHOG000034038.
HOVERGENHBG060279.
InParanoidP31955.
KOK09782.
OMAEYEGEAE.
OrthoDBEOG7XDBH6.
PhylomeDBP31955.
TreeFamTF332773.

Gene expression databases

ArrayExpressP31955.
BgeeP31955.
CleanExMM_AREG.
GenevestigatorP31955.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR015497. EGF_rcpt_ligand.
[Graphical view]
PANTHERPTHR10740. PTHR10740. 1 hit.
SMARTSM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279779.
PMAP-CutDBP31955.
PROP31955.
SOURCESearch...

Entry information

Entry nameAREG_MOUSE
AccessionPrimary (citable) accession number: P31955
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot