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Protein

Diacylglycerol acyltransferase/mycolyltransferase Ag85B

Gene

fbpB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).By similarity

Catalytic activityi

2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate.
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164NucleophileBy similarity1
Binding sitei164SubstrateBy similarity1
Binding sitei192SubstrateBy similarity1
Active sitei268By similarity1
Binding sitei277SubstrateBy similarity1
Active sitei300By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Protein family/group databases

ESTHERimycle-a85b. A85-Mycolyl-transferase.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol acyltransferase/mycolyltransferase Ag85B (EC:2.3.1.122, EC:2.3.1.20)
Short name:
DGAT
Alternative name(s):
30 kDa extracellular protein
Acyl-CoA:diacylglycerol acyltransferase
Antigen 85 complex B
Short name:
85B
Short name:
Ag85B
Extracellular alpha-antigen
Fibronectin-binding protein B
Short name:
Fbps B
Gene namesi
Name:fbpB
Ordered Locus Names:ML2028
ORF Names:MLCB561.03c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML2028.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 38Sequence analysisAdd BLAST38
ChainiPRO_000000022039 – 327Diacylglycerol acyltransferase/mycolyltransferase Ag85BAdd BLAST289

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi125 ↔ 130By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi272631.ML2028.

Structurei

3D structure databases

ProteinModelPortaliP31951.
SMRiP31951.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 81Substrate bindingBy similarity2
Regioni96 – 106Fibronectin-bindingAdd BLAST11
Regioni270 – 273Substrate bindingBy similarity4
Regioni300 – 302Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the mycobacterial A85 antigen family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C4W. Bacteria.
COG0627. LUCA.
HOGENOMiHOG000049505.
KOiK18851.
OMAiMSGSSAM.
OrthoDBiPOG091H0560.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDVSGKIRA WGRWLLVGAA ATLPSLISLA GGAATASAFS RPGLPVEYLQ
60 70 80 90 100
VPSEAMGRSI KVQFQNGGNG SPAVYLLDGL RAQDDYNGWD INTSAFEWYY
110 120 130 140 150
QSGLSVVMPV GGQSSFYSDW YSPACGKAGC TTYKWETFLT SELPKWLSAN
160 170 180 190 200
RSVKSTGSAV VGLSMAGSSA LILAAYHPDQ FIYAGSLSAL MDSSQGIEPQ
210 220 230 240 250
LIGLAMGDAG GYKAADMWGP PNDPAWQRND PILQAGKLVA NNTHLWVYCG
260 270 280 290 300
NGTPSELGGT NVPAEFLENF VHGSNLKFQD AYNGAGGHNA VFNLNADGTH
310 320
SWEYWGAQLN AMKPDLQNTL MAVPRSG
Length:327
Mass (Da):34,807
Last modified:April 27, 2001 - v2
Checksum:i2B0EB9B37AAF985C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59S → T in CAA43269 (PubMed:1945858).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60934 Genomic DNA. Translation: CAA43269.1.
AL049571 Genomic DNA. Translation: CAB40285.1.
AL583924 Genomic DNA. Translation: CAC30983.1.
PIRiG87162.
S20038.
S34434.
RefSeqiNP_302359.1. NC_002677.1.
WP_010908679.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30983; CAC30983; CAC30983.
GeneIDi909036.
KEGGimle:ML2028.
PATRICi18057796. VBIMycLep78757_3822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60934 Genomic DNA. Translation: CAA43269.1.
AL049571 Genomic DNA. Translation: CAB40285.1.
AL583924 Genomic DNA. Translation: CAC30983.1.
PIRiG87162.
S20038.
S34434.
RefSeqiNP_302359.1. NC_002677.1.
WP_010908679.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP31951.
SMRiP31951.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML2028.

Protein family/group databases

ESTHERimycle-a85b. A85-Mycolyl-transferase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30983; CAC30983; CAC30983.
GeneIDi909036.
KEGGimle:ML2028.
PATRICi18057796. VBIMycLep78757_3822.

Organism-specific databases

LepromaiML2028.

Phylogenomic databases

eggNOGiENOG4105C4W. Bacteria.
COG0627. LUCA.
HOGENOMiHOG000049505.
KOiK18851.
OMAiMSGSSAM.
OrthoDBiPOG091H0560.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
[Graphical view]
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA85B_MYCLE
AccessioniPrimary (citable) accession number: P31951
Secondary accession number(s): Q9RIA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.