P31944 (CASPE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caspase-14
Short name=CASP-14
EC=3.4.22.-

Cleaved into the following 2 chains:

1. Caspase-14 subunit p19
2. Caspase-14 subunit p10

Gene names

Name:

CASP14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin By similarity.
Subunit structure	Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.5 Ref.6.
Tissue specificity	Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels. Ref.5
Induction	In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Ref.5
Sequence similarities	Belongs to the peptidase C14A family.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: InterPro cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW epidermis development Traceable author statement PubMed 10203698. Source: ProtInc proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell keratin filament Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type endopeptidase activity Traceable author statement PubMed 10203698. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – ?		Potential		PRO_0000004652
	Chain	? – 152		Caspase-14 subunit p19		PRO_0000004653
	Chain	153 – 242	90	Caspase-14 subunit p10		PRO_0000004654
Sites
	Active site	89	1	By similarity
	Active site	132	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31944 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: E539FB7E8DD808A2	FASTA	242	27,680
	10 20 30 40 50 60 MSNPRSLEEE KYDMSGARLA LILCVTKARE GSEEDLDALE HMFRQLRFES TMKRDPTAEQ 70 80 90 100 110 120 FQEELEKFQQ AIDSREDPVS CAFVVLMAHG REGFLKGEDG EMVKLENLFE ALNNKNCQAL 130 140 150 160 170 180 RAKPKVYIIQ ACRGEQRDPG ETVGGDEIVM VIKDSPQTIP TYTDALHVYS TVEGYIAYRH 190 200 210 220 230 240 DQKGSCFIQT LVDVFTKRKG HILELLTEVT RRMAEAELVQ EGKARKTNPE IQSTLRKRLY LQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caspase-14: analysis of gene structure and mRNA expression during keratinocyte differentiation." Eckhart L., Ban J., Fischer H., Tschachler E. Biochem. Biophys. Res. Commun. 277:655-659(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression and transcriptional regulation of caspase-14 in simple and complex epithelia." Pistritto G., Jost M., Srinivasula S.M., Baffa R., Poyet J.-L., Kari C., Lazebnik Y., Rodeck U., Alnemri E.S. Cell Death Differ. 9:995-1006(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes." Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J. Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 68-74; 137-147 AND 154-162. Tissue: Keratinocyte.
[5]	"Epidermal differentiation does not involve the pro-apoptotic executioner caspases, but is associated with caspase-14 induction and processing." Lippens S., Kockx M., Knaapen M., Mortier L., Polakowska R., Verheyen A., Garmyn M., Zwijsen A., Formstecher P., Huylebroeck D., Vandenabeele P., Declercq W. Cell Death Differ. 7:1218-1224(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[6]	"Processing of native caspase-14 occurs at an atypical cleavage site in normal epidermal differentiation." Chien A.J., Presland R.B., Kuechle M.K. Biochem. Biophys. Res. Commun. 296:911-917(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 153-163, PROTEOLYTIC PROCESSING, SUBUNIT, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF097874 mRNA. Translation: AAD16173.1. BC069541 mRNA. Translation: AAH69541.1. BC103868 mRNA. Translation: AAI03869.1. BC103869 mRNA. Translation: AAI03870.1.
IPI	IPI00013885.
PIR	JC7517.
RefSeq	NP_036246.1. NM_012114.2.
UniGene	Hs.466057.
3D structure databases
ProteinModelPortal	P31944.
ModBase	Search...
Protein-protein interaction databases
IntAct	P31944. 3 interactions.
STRING	9606.ENSP00000221740.
Protein family/group databases
MEROPS	C14.018.
Polymorphism databases
DMDM	12231007.
Proteomic databases
PaxDb	P31944.
PeptideAtlas	P31944.
PRIDE	P31944.
Protocols and materials databases
DNASU	23581.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000221740; ENSP00000221740; ENSG00000105141. ENST00000427043; ENSP00000393417; ENSG00000105141.
GeneID	23581.
KEGG	hsa:23581.
UCSC	uc010dzv.2. human.
Organism-specific databases
CTD	23581.
GeneCards	GC19P015160.
HGNC	HGNC:1502. CASP14.
HPA	CAB010059.
MIM	605848. gene.
neXtProt	NX_P31944.
PharmGKB	PA26085.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG327631.
HOGENOM	HOG000231878.
HOVERGEN	HBG050804.
InParanoid	P31944.
KO	K04401.
OMA	CVTKARE.
OrthoDB	EOG4HX51W.
PhylomeDB	P31944.
Gene expression databases
ArrayExpress	P31944.
Bgee	P31944.
CleanEx	HS_CASP14.
Genevestigator	P31944.
GermOnline	ENSG00000105141. Homo sapiens.
Family and domain databases
InterPro	IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
PANTHER	PTHR10454. PTHR10454. 1 hit.
Pfam	PF00656. Peptidase_C14. 1 hit. [Graphical view]
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00115. CASc. 1 hit. [Graphical view]
PROSITE	PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. False negative. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P31944.
ChEMBL	CHEMBL5991.
GenomeRNAi	23581.
NextBio	46186.
PMAP-CutDB	P31944.
SOURCE	Search...

Entry information

Entry name

CASPE_HUMAN

Accession

Primary (citable) accession number: P31944
Secondary accession number(s): O95823, Q3SYC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.