Dual specificity mitogen-activated protein kinase kinase 1

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Reviewed, UniProtKB/Swiss-Prot P31938 (MP2K1_MOUSE)

Last modified November 3, 2009. Version 99. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 1
      Short name=MAP kinase kinase 1
      Short name=MAPKK 1
    EC=2.7.12.2
Alternative name(s):
    ERK activator kinase 1
    MAPK/ERK kinase 1
      Short name=MEK1

Gene names

Name:	Map2k1
Synonyms:	Mek1, Prkmk1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Inhibited by serine/threonine phosphatase 2A.
Subunit structure	Interacts with MORG1. Ref.6
Post-translational modification	MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

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Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	activation of MAPK activity Inferred from mutant phenotype. Source: MGI cell motility Inferred from mutant phenotype. Source: MGI keratinocyte differentiation Inferred from mutant phenotype. Source: MGI labyrinthine layer development Inferred from mutant phenotype. Source: MGI neuron differentiation Inferred from mutant phenotype. Source: MGI placenta blood vessel development Inferred from mutant phenotype. Source: MGI positive regulation of cell differentiation Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase activity Inferred from mutant phenotype. Source: MGI protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW receptor signaling protein tyrosine phosphatase activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 393

392

Dual specificity mitogen-activated protein kinase kinase 1

PRO_0000086366

Regions

Domain

68 – 361

294

Protein kinase

Nucleotide binding

74 – 82

ATP By similarity

Compositional bias

262 – 307

Pro-rich

Sites

Active site

190

Proton acceptor By similarity

Binding site

ATP By similarity

Site

8 – 9

Cleavage; by anthrax lethal factor By similarity

Amino acid modifications

Modified residue

218

Phosphoserine; by RAF By similarity

Modified residue

222

Phosphoserine; by RAF By similarity

Modified residue

231

Phosphoserine By similarity

Modified residue

286

Phosphothreonine By similarity

Modified residue

386

Phosphothreonine By similarity

Experimental info

Sequence conflict

374

W → Q AA sequence Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

P31938-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 01D1D18572AE40E7
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FASTA

393

43,474

        10         20         30         40         50         60 
MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV 

        70         80         90        100        110        120 
GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE 

       130        140        150        160        170        180 
CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL 

       190        200        210        220        230        240 
REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY 

       250        260        270        280        290        300 
SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELLFGCHVE GDAAETPPRP RTPGRPLSSY 

       310        320        330        340        350        360 
GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF 

       370        380        390 
IKRSDAEEVD FAGWLCSTIG LNQPSTPTHA ASI

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." Crews C.M., Alessandrini A., Erikson R.L. Science 258:478-480(1992) [PubMed: 1411546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product." Crews C.M., Erikson R.L. Proc. Natl. Acad. Sci. U.S.A. 89:8205-8209(1992) [PubMed: 1381507] [Abstract] Cited for: PROTEIN SEQUENCE OF 4-20; 71-84; 114-136; 206-234 AND 363-384. Tissue: T-cell.
[4]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 206-227; 270-291 AND 325-340, MASS SPECTROMETRY. Tissue: Hippocampus.
[5]	"Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor." Duesbery N.S., Webb C.P., Leppla S.H., Gordon V.M., Klimpel K.R., Copeland T.D., Ahn N.G., Oskarsson M.K., Fukasawa K., Paull K.D., Vande Woude G.F. Science 280:734-737(1998) [PubMed: 9563949] [Abstract] Cited for: CLEAVAGE BY ANTHRAX LETHAL FACTOR.
[6]	"Modular construction of a signaling scaffold: MORG1 interacts with components of the ERK cascade and links ERK signaling to specific agonists." Vomastek T., Schaeffer H.-J., Tarcsafalvi A., Smolkin M.E., Bissonette E.A., Weber M.J. Proc. Natl. Acad. Sci. U.S.A. 101:6981-6986(2004) [PubMed: 15118098] [Abstract] Cited for: INTERACTION WITH MORG1.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	L02526 mRNA. Translation: AAA39523.1. BC054754 mRNA. Translation: AAH54754.1.
IPI	IPI00466610.
PIR	I59571.
RefSeq	NP_032953.1.
UniGene	Mm.248907
3D structure databases
HSSP	HSSP built from PDB template 1BI8 based on UniProtKB Q00534.
SMR	P31938. Positions 62-382.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:467N.
STRING	P31938.
PTM databases
PhosphoSite	P31938.
Proteomic databases
PRIDE	P31938.
Genome annotation databases
Ensembl	ENSMUST00000005066; ENSMUSP00000005066; ENSMUSG00000004936; Mus musculus. [Genome view]
GeneID	26395.
KEGG	mmu:26395.
UCSC	uc009qbp.1. mouse.
Organism-specific databases
CTD	26395.
MGI	MGI:1346866. Map2k1.
Phylogenomic databases
HOGENOM	P31938.
HOVERGEN	P31938.
OMA	MQKRRKP.
Enzyme and pathway databases
BRENDA	2.7.12.2. 244.
Gene expression databases
ArrayExpress	P31938.
Bgee	P31938.
CleanEx	MM_MAP2K1.
Genevestigator	P31938.
GermOnline	ENSMUSG00000004936. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	304339.
SOURCE	Search...

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Entry information

Entry name

MP2K1_MOUSE

Accession

Primary (citable) accession number: P31938

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents