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Protein

Sucrose synthase 1

Gene

SS1

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.1 Publication

Catalytic activityi

NDP-glucose + D-fructose = NDP + sucrose.1 Publication

Kineticsi

  1. KM=30 mM for Sucrose1 Publication
  2. KM=5 µM for UDP1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.13. 2687.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sucrose synthase 1 (EC:2.4.1.13)
    Alternative name(s):
    Sucrose-UDP glucosyltransferase 1
    Gene namesi
    Name:SS1
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

    Organism-specific databases

    GrameneiP31922.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 807807Sucrose synthase 1PRO_0000204649Add
    BLAST

    Expressioni

    Tissue specificityi

    Highly expressed in developing endosperm and in roots and, at lower levels, in coleoptiles and aleurone. In 3 day old roots it is detected in cap cells and along the vascular strand, starting just after the meristemic region. In 9 day old leaves it is found in the phloem. In seeds it is distributed throughout the endosperm and also found in the assimilate-unloading tissues, the nucellar projection, the vascular area and at a high concentration in the chalazal region.1 Publication

    Developmental stagei

    Activity increases as seeds develop.1 Publication

    Interactioni

    Subunit structurei

    Forms homotetramers. In endosperm it forms both homotetramers and heterotetramers with SS2, all three possible heterotetramers are formed.1 Publication

    Protein-protein interaction databases

    STRINGi4513.MLOC_11770.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP31922.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR000368. Sucrose_synth.
    IPR012820. Sucrose_synthase_pln/cyn.
    [Graphical view]
    PfamiPF00534. Glycos_transf_1. 1 hit.
    PF00862. Sucrose_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02470. sucr_synth. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P31922-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAAKLTRLHS LRERLGATFS SHPNELIALF SRYVHQGKGM LQRHQLLAEF
    60 70 80 90 100
    DALFESDKEK YAPFEDILRA AQEAIVLPPW VALAIRPRTG VWDYIRVNVS
    110 120 130 140 150
    ELAVEELTVS EYLAFKEQLV DEHASRKFVL ELDFEPFNAS FPRPSMSKSY
    160 170 180 190 200
    GKGVQFLNRH LSSKLFQDKE SLYPLLNFLK AHNYKGTTMI LNDRIQSLRG
    210 220 230 240 250
    LQSALRKAEE YLVSIPEDTP SSEFNHRFQE LGLEKGWGDT AKRVHDTIHL
    260 270 280 290 300
    LLDLLEAPDP ASLEKFLGTI PMMFNVVILS PHGYFAQSNV LGYPDTGGQV
    310 320 330 340 350
    VYILDQVRAL ENEMLLRIKQ QGLDITPKIL IVTRLLPDAV GTTCGQRLEK
    360 370 380 390 400
    VIGTEHTDIL RVPFRTENGI RKWISRFDVW PYLETYTEDV ANELMREMQT
    410 420 430 440 450
    KPDLIIGNYS DGNLVATLLA HKLGVTQCTI AHALEKTKYP NSDIYLDKFD
    460 470 480 490 500
    SQYHFSCQFT ADLIAMNHTD FIITSTFQEI AGSKDSVGQY ESHIAFTLPD
    510 520 530 540 550
    LYRVVHGIDV FDPKFNIVSP GADMTVYFPY TETDKRLTAF HSEIEELLYS
    560 570 580 590 600
    DVENDEHKFV LKDRNKPIIF SMARLDRVKN MTGLVEMYGK NAHLKDLANL
    610 620 630 640 650
    VIVAGDHGKE SKDREEQAEF KRMYSLIEEY KLKGHIRWIS AQMNRVRNGE
    660 670 680 690 700
    LYRYICDTKG AFVQPAFYEA FGLTVIEAMT CGLPTIATCH GGPAEIIVDG
    710 720 730 740 750
    VSGLHIDPYH SDKAADILVN FFEKSTADPS YWDKISQGGL KRIYEKYTWK
    760 770 780 790 800
    LYSERLMTLT GVYGFWKYVS NLERRETRRY LEMFYALKYR SLAAAVPLAV

    DGESSGN
    Length:807
    Mass (Da):92,211
    Last modified:July 1, 1993 - v1
    Checksum:iA863A8C876A060C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti370 – 3701I → IL in CAA47264 (Ref. 2) Curated
    Sequence conflicti374 – 3741I → Y in CAA47264 (Ref. 2) Curated
    Sequence conflicti392 – 3932NE → KQ in CAA47264 (Ref. 2) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X65871 mRNA. Translation: CAA46701.1.
    X66728 mRNA. Translation: CAA47264.1.
    PIRiS29242.
    UniGeneiHv.639.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X65871 mRNA. Translation: CAA46701.1.
    X66728 mRNA. Translation: CAA47264.1.
    PIRiS29242.
    UniGeneiHv.639.

    3D structure databases

    ProteinModelPortaliP31922.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi4513.MLOC_11770.1.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Organism-specific databases

    GrameneiP31922.

    Enzyme and pathway databases

    BRENDAi2.4.1.13. 2687.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR000368. Sucrose_synth.
    IPR012820. Sucrose_synthase_pln/cyn.
    [Graphical view]
    PfamiPF00534. Glycos_transf_1. 1 hit.
    PF00862. Sucrose_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02470. sucr_synth. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "Homologous sucrose synthase genes in barley (Hordeum vulgare) are located in chromosomes 7H (syn. 1) and 2H. Evidence for a gene translocation?"
      Sanchez de la Hoz P., Vicente-Carbajosa J., Mena M., Carbonero P.
      FEBS Lett. 310:46-50(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Abyssinian 2231.
      Tissue: Endosperm.
    2. Brandt J., Thordal-Christensen H., Collinge D.B.
      Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 223-807.
      Strain: cv. Pallas.
    3. "The spatial distribution of sucrose synthase isozymes in barley."
      Guerin J., Carbonero P.
      Plant Physiol. 114:55-62(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY.
    4. "Sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants."
      Baroja-Fernandez E., Munoz F.J., Saikusa T., Rodriguez-Lopez M., Akazawa T., Pozueta-Romero J.
      Plant Cell Physiol. 44:500-509(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiSUS1_HORVU
    AccessioniPrimary (citable) accession number: P31922
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: July 22, 2015
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.