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Protein

Sucrose synthase 1

Gene

SS1

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.1 Publication

Catalytic activityi

NDP-glucose + D-fructose = NDP + sucrose.1 Publication

Kineticsi

  1. KM=30 mM for Sucrose1 Publication
  2. KM=5 µM for UDP1 Publication

GO - Molecular functioni

  1. sucrose synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. biosynthetic process Source: InterPro
  2. sucrose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrose synthase 1 (EC:2.4.1.13)
Alternative name(s):
Sucrose-UDP glucosyltransferase 1
Gene namesi
Name:SS1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP31922.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 807807Sucrose synthase 1PRO_0000204649Add
BLAST

Expressioni

Tissue specificityi

Highly expressed in developing endosperm and in roots and, at lower levels, in coleoptiles and aleurone. In 3 day old roots it is detected in cap cells and along the vascular strand, starting just after the meristemic region. In 9 day old leaves it is found in the phloem. In seeds it is distributed throughout the endosperm and also found in the assimilate-unloading tissues, the nucellar projection, the vascular area and at a high concentration in the chalazal region.1 Publication

Developmental stagei

Activity increases as seeds develop.1 Publication

Gene expression databases

ExpressionAtlasiP31922. baseline.
GenevestigatoriP31922.

Interactioni

Subunit structurei

Forms homotetramers. In endosperm it forms both homotetramers and heterotetramers with SS2, all three possible heterotetramers are formed.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP31922.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR000368. Sucrose_synth.
IPR012820. Sucrose_synthase_pln/cyn.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02470. sucr_synth. 1 hit.

Sequencei

Sequence statusi: Complete.

P31922-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKLTRLHS LRERLGATFS SHPNELIALF SRYVHQGKGM LQRHQLLAEF
60 70 80 90 100
DALFESDKEK YAPFEDILRA AQEAIVLPPW VALAIRPRTG VWDYIRVNVS
110 120 130 140 150
ELAVEELTVS EYLAFKEQLV DEHASRKFVL ELDFEPFNAS FPRPSMSKSY
160 170 180 190 200
GKGVQFLNRH LSSKLFQDKE SLYPLLNFLK AHNYKGTTMI LNDRIQSLRG
210 220 230 240 250
LQSALRKAEE YLVSIPEDTP SSEFNHRFQE LGLEKGWGDT AKRVHDTIHL
260 270 280 290 300
LLDLLEAPDP ASLEKFLGTI PMMFNVVILS PHGYFAQSNV LGYPDTGGQV
310 320 330 340 350
VYILDQVRAL ENEMLLRIKQ QGLDITPKIL IVTRLLPDAV GTTCGQRLEK
360 370 380 390 400
VIGTEHTDIL RVPFRTENGI RKWISRFDVW PYLETYTEDV ANELMREMQT
410 420 430 440 450
KPDLIIGNYS DGNLVATLLA HKLGVTQCTI AHALEKTKYP NSDIYLDKFD
460 470 480 490 500
SQYHFSCQFT ADLIAMNHTD FIITSTFQEI AGSKDSVGQY ESHIAFTLPD
510 520 530 540 550
LYRVVHGIDV FDPKFNIVSP GADMTVYFPY TETDKRLTAF HSEIEELLYS
560 570 580 590 600
DVENDEHKFV LKDRNKPIIF SMARLDRVKN MTGLVEMYGK NAHLKDLANL
610 620 630 640 650
VIVAGDHGKE SKDREEQAEF KRMYSLIEEY KLKGHIRWIS AQMNRVRNGE
660 670 680 690 700
LYRYICDTKG AFVQPAFYEA FGLTVIEAMT CGLPTIATCH GGPAEIIVDG
710 720 730 740 750
VSGLHIDPYH SDKAADILVN FFEKSTADPS YWDKISQGGL KRIYEKYTWK
760 770 780 790 800
LYSERLMTLT GVYGFWKYVS NLERRETRRY LEMFYALKYR SLAAAVPLAV

DGESSGN
Length:807
Mass (Da):92,211
Last modified:July 1, 1993 - v1
Checksum:iA863A8C876A060C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti370 – 3701I → IL in CAA47264 (Ref. 2) Curated
Sequence conflicti374 – 3741I → Y in CAA47264 (Ref. 2) Curated
Sequence conflicti392 – 3932NE → KQ in CAA47264 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65871 mRNA. Translation: CAA46701.1.
X66728 mRNA. Translation: CAA47264.1.
PIRiS29242.
UniGeneiHv.639.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65871 mRNA. Translation: CAA46701.1.
X66728 mRNA. Translation: CAA47264.1.
PIRiS29242.
UniGeneiHv.639.

3D structure databases

ProteinModelPortaliP31922.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP31922.

Gene expression databases

ExpressionAtlasiP31922. baseline.
GenevestigatoriP31922.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR000368. Sucrose_synth.
IPR012820. Sucrose_synthase_pln/cyn.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02470. sucr_synth. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Homologous sucrose synthase genes in barley (Hordeum vulgare) are located in chromosomes 7H (syn. 1) and 2H. Evidence for a gene translocation?"
    Sanchez de la Hoz P., Vicente-Carbajosa J., Mena M., Carbonero P.
    FEBS Lett. 310:46-50(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Abyssinian 2231.
    Tissue: Endosperm.
  2. Brandt J., Thordal-Christensen H., Collinge D.B.
    Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 223-807.
    Strain: cv. Pallas.
  3. "The spatial distribution of sucrose synthase isozymes in barley."
    Guerin J., Carbonero P.
    Plant Physiol. 114:55-62(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY.
  4. "Sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants."
    Baroja-Fernandez E., Munoz F.J., Saikusa T., Rodriguez-Lopez M., Akazawa T., Pozueta-Romero J.
    Plant Cell Physiol. 44:500-509(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiSUS1_HORVU
AccessioniPrimary (citable) accession number: P31922
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: January 7, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.