Uptake hydrogenase small subunit precursor - Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

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Reviewed, UniProtKB/Swiss-Prot P31892 (MBHS_RALEH)

Last modified June 16, 2009. Version 76. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Uptake hydrogenase small subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase small subunit

Gene names

Name:	hoxK
Ordered Locus Names:	PHG001

Encoded on

Plasmid megaplasmid pHG1

Organism

Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

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Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This enzyme recycles the H₂ produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O₂ under carbon- or phosphate-limited conditions.
Catalytic activity	H₂ + A = AH₂.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

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Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 43

Tat-type signal Ref.3

Chain

44 – 360

317

Uptake hydrogenase small subunit

PRO_0000013421

Sites

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

158

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

192

Iron-sulfur 1 (4Fe-4S) By similarity

Metal binding

230

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

233

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

258

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

264

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

273

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

292

Iron-sulfur 3 (3Fe-4S) By similarity

Metal binding

295

Iron-sulfur 3 (3Fe-4S) By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P31892-1 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: D2BB15D85902AF0C
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FASTA

360

39,472

        10         20         30         40         50         60 
MVETFYEVMR RQGISRRSFL KYCSLTATSL GLGPSFLPQI AHAMETKPRT PVLWLHGLEC 

        70         80         90        100        110        120 
TCCSESFIRS AHPLAKDVVL SMISLDYDDT LMAAAGHQAE AILEEIMTKY KGNYILAVEG 

       130        140        150        160        170        180 
NPPLNQDGMS CIIGGRPFIE QLKYVAKDAK AIISWGSCAS WGCVQAAKPN PTQATPVHKV 

       190        200        210        220        230        240 
ITDKPIIKVP GCPPIAEVMT GVITYMLTFD RIPELDRQGR PKMFYSQRIH DKCYRRPHFD 

       250        260        270        280        290        300 
AGQFVEEWDD ESARKGFCLY KMGCKGPTTY NACSTTRWNE GTSFPIQSGH GCIGCSEDGF 

       310        320        330        340        350        360 
WDKGSFYDRL TGISQFGVEA NADKIGGTAS VVVGAAVTAH AAASAIKRAS KKNETSGSEH

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16." Kortlueke C., Horstmann K., Schwartz E., Rohde M., Binsack R., Friedrich B. J. Bacteriol. 174:6277-6289(1992) [PubMed: 1383192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis." Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G. J. Mol. Biol. 332:369-383(2003) [PubMed: 12948488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria." Lorenz B., Schneider K., Kratzin H., Schlegel H.G. Biochim. Biophys. Acta 995:1-9(1989) [PubMed: 2493816] [Abstract] Cited for: PROTEIN SEQUENCE OF 44-59.

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Cross-references

Sequence databases
	M96433 Unassigned DNA. Translation: AAA16461.1. AY305378 Genomic DNA. Translation: AAP85757.1.
PIR	A43255.
RefSeq	NP_942643.1.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Genome annotation databases
GeneID	2656804.
GenomeReviews	Gene locus PHG001 in contig AY305378_GR.
KEGG	reh:PHG001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P31892.
OMA	P31892. FYEVMRR.
Enzyme and pathway databases
BioCyc	MetaCyc:HOXKALCA-MON.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

MBHS_RALEH

Accession

Primary (citable) accession number: P31892

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents