Reviewed,
UniProtKB/Swiss-Prot P31891 (MBHL_RALEH)
Last modified
June 16, 2009.
Version 66.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Uptake hydrogenase large subunit EC=1.12.99.6 Alternative name(s): Hydrogenlyase Membrane-bound hydrogenase large subunit | ||||
| Gene names |
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| Encoded on | Plasmid megaplasmid pHG1 | ||||
| Organism | Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 381666 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus |
Protein attributes
| Sequence length | 618 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions. |
| Catalytic activity | H2 + A = AH2. |
| Cofactor | Binds 1 nickel ion per subunit By similarity. |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Subcellular location | |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Ligand | Metal-binding Nickel |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing Plasmid |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activityInferred from electronic annotation. Source: EC nickel ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.3 | ||||||
| Chain | 2 – 618 | 617 | Uptake hydrogenase large subunit | PRO_0000199706 | |||||
Sites | |||||||||
| Metal binding | 75 | 1 | Nickel Potential | ||||||
| Metal binding | 78 | 1 | Nickel Potential | ||||||
| Metal binding | 597 | 1 | Nickel Potential | ||||||
| Metal binding | 600 | 1 | Nickel Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 578 | 1 | M → V in AAA16462. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16." Kortlueke C., Horstmann K., Schwartz E., Rohde M., Binsack R., Friedrich B. J. Bacteriol. 174:6277-6289(1992) [PubMed: 1383192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis." Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G. J. Mol. Biol. 332:369-383(2003) [PubMed: 12948488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria." Lorenz B., Schneider K., Kratzin H., Schlegel H.G. Biochim. Biophys. Acta 995:1-9(1989) [PubMed: 2493816] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-31. |
Cross-references
Sequence databases | |
|---|---|
| M96433 Unassigned DNA. Translation: AAA16462.1. AY305378 Genomic DNA. Translation: AAP85758.1. | |
| PIR | B43255. |
| RefSeq | NP_942644.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1UBL based on UniProtKB P21852. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2656805. |
| GenomeReviews | Gene locus PHG002 in contig AY305378_GR. |
| KEGG | reh:PHG002. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P31891. |
| OMA | P31891. VLPPEAN. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:HOXGALCA-MON. |
Family and domain databases | |
| InterPro | IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view] |
| Pfam | PF00374. NiFeSe_Hases. 1 hit. [Graphical view] |
| PROSITE | PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MBHL_RALEH | ||||||||
| Accession | Primary (citable) accession number: P31891 Secondary accession number(s): Q7WXU5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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