P31891 (MBHL_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uptake hydrogenase large subunit
EC=1.12.99.6

Alternative name(s):
Hydrogenlyase
Membrane-bound hydrogenase large subunit

Gene names

Name:	hoxG
Ordered Locus Names:	PHG002

Encoded on

Plasmid megaplasmid pHG1

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	618 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 618	617	Uptake hydrogenase large subunit		PRO_0000199706
Sites
	Metal binding	75	1	Nickel Potential
	Metal binding	78	1	Nickel Potential
	Metal binding	597	1	Nickel Potential
	Metal binding	600	1	Nickel Potential
Experimental info
	Sequence conflict	578	1	M → V in AAA16462. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31891 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 8899BA7486865D3F	FASTA	618	68,795
	10 20 30 40 50 60 MSAYATQGFN LDDRGRRIVV DPVTRIEGHM RCEVNVDANN VIRNAVSTGT MWRGLEVILK 70 80 90 100 110 120 GRDPRDAWAF VERICGVCTG CHALASVRAV ENALDIRIPK NAHLIREIMA KTLQVHDHAV 130 140 150 160 170 180 HFYHLHALDW VDVMSALKAD PKRTSELQQL VSPAHPLSSA GYFRDIQNRL KRFVESGQLG 190 200 210 220 230 240 PFMNGYWGSK AYVLPPEANL MAVTHYLEAL DLQKEWVKIH TIFGGKNPHP NYLVGGVPCA 250 260 270 280 290 300 INLDGIGAAS APVNMERLSF VKARIDEIIE FNKNVYVPDV LAIGTLYKQA GWLYGGGLAA 310 320 330 340 350 360 TNVLDYGEYP NVAYNKSTDQ LPGGAILNGN WDEVFPVDPR DSQQVQEFVS HSWYKYADES 370 380 390 400 410 420 VGLHPWDGVT EPNYVLGANT KGTRTRIEQI DESAKYSWIK SPRWRGHAME VGPLSRYILA 430 440 450 460 470 480 YAHARSGNKY AERPKEQLEY SAQMINSAIP KALGLPETQY TLKQLLPSTI GRTLARALES 490 500 510 520 530 540 QYCGEMMHSD WHDLVANIRA GDTATANVDK WDPATWPLQA KGVGTVAAPR GALGHWIRIK 550 560 570 580 590 600 DGRIENYQCV VPTTWNGSPR DYKGQIGAFE ASLMNTPMVN PEQPVEILRT LHSFDPCLAC 610 STHVMSAEGQ ELTTVKVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16." Kortlueke C., Horstmann K., Schwartz E., Rohde M., Binsack R., Friedrich B. J. Bacteriol. 174:6277-6289(1992) [PubMed: 1383192] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis." Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G. J. Mol. Biol. 332:369-383(2003) [PubMed: 12948488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.
[3]	"Immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria." Lorenz B., Schneider K., Kratzin H., Schlegel H.G. Biochim. Biophys. Acta 995:1-9(1989) [PubMed: 2493816] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-31.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M96433 Unassigned DNA. Translation: AAA16462.1.
AY305378 Genomic DNA. Translation: AAP85758.1.

PIR

B43255.

RefSeq

NP_942644.1. NC_005241.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RGW	X-ray	1.50	L	1-603	[»]

ProteinModelPortal

P31891.

ModBase

Search...

Protein-protein interaction databases

STRING

P31891.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2656805.

GenomeReviews

Gene locus PHG002 in contig AY305378_GR.

KEGG

reh:PHG002.

PATRIC

35228660. VBIRalEut6770_0002.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG733231.

OMA

DGVTEPN.

PhylomeDB

P31891.

ProtClustDB

CLSK923789.

Enzyme and pathway databases

BioCyc

MetaCyc:HOXGALCA-MONOMER.
REUT381666:PHG002-MONOMER.

Family and domain databases

InterPro

IPR001501. Ni-dep_hyd_lsu.
IPR018194. Ni-dep_hyd_lsu_Ni_BS.
[Graphical view]

KO

K06281.

Pfam

PF00374. NiFeSe_Hases. 1 hit.
[Graphical view]

PROSITE

PS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MBHL_CUPNH

Accession

Primary (citable) accession number: P31891
Secondary accession number(s): Q7WXU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 84 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program