Reviewed, UniProtKB/Swiss-Prot P31884 (MBHS_WOLSU)

Names and origin

Protein names

Recommended name:
    Quinone-reactive Ni/Fe-hydrogenase small chain
    EC=1.12.5.1
Alternative name(s):
    Membrane-bound hydrogenase small subunit
    Hydrogen:quinone oxidoreductase

Gene names

Name:	hydA
Ordered Locus Names:	WS1687

Organism

Wolinella succinogenes [Complete proteome] [HAMAP]

Taxonomic identifier

844 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	H2 + menaquinone = menaquinol.
Cofactor	Binds 1 3Fe-4S cluster By similarity. Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Ligand	3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ferredoxin hydrogenase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogen:quinone oxidoreductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Tat-type signal Ref.1
	Chain	37 – 354	318	Quinone-reactive Ni/Fe-hydrogenase small chain		PRO_0000013437
Sites
	Metal binding	53	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	153	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	186	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	224	1	Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
	Metal binding	227	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	252	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	258	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	267	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	286	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	289	1	Iron-sulfur 3 (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31884-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: C39CC21D2F5AB7D0	FASTA	354	38,294
	10 20 30 40 50 60 MLEEKGIERR DFMKWAGAMT AMLSLPATFT PLTAKAAELA DRLPVIWLHM AECTGCSESL 70 80 90 100 110 120 LRTDGPGIDS LIFDYISLEY HETVMAAAGW QAEHNLEHAI EKYKGRYVLM VEGGIPAGSS 130 140 150 160 170 180 EFYLTVGPHG TTGAEHARHA SANAAAIFAI GSCSSFGGVQ AARPNPTNAQ PLSKVTNKPV 190 200 210 220 230 240 INVPGCPPSE KNIVGNVLHF ILFGTLPSVD AFNRPMWAYG LRIHDLCERR GRFDAGEFVQ 250 260 270 280 290 300 EFGDEGAKKG YCLYKVGCKG PYTFNNCSKL RFNQHTSWPV QAGHGCIGCS EPDFWDTMGP 310 320 330 340 350 FEEPVANRLY ATAFDGLGAD KTADKIGITL LAATAVGVAA HAVLSMMVKD KENN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes." Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A. Eur. J. Biochem. 206:93-102(1992) [PubMed: 1587288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 37-52.
[2]	Erratum Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A. Eur. J. Biochem. 214:949-950(1993) [PubMed: 8319698] [Abstract]
[3]	"Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed: 14500908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSMZ 1740.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65189 Genomic DNA. Translation: CAA46302.1. Different initiation. BX571661 Genomic DNA. Translation: CAE10714.1. Different initiation.
PIR	S33852.
RefSeq	NP_907814.1.
3D structure databases
HSSP	HSSP built from PDB template 1FRF based on UniProtKB P18187.
ModBase	Search...
Genome annotation databases
GeneID	2554748.
GenomeReviews	Gene locus WS1687 in contig BX571656_GR.
KEGG	wsu:WS1687.
NMPDR	fig|273121.1.peg.1541.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P31884.
Enzyme and pathway databases
BioCyc	WSUC273121:WS1687-MON.
BRENDA	1.12.5.1. 191624.
Family and domain databases
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001821. NiFe_hydrogenase_ssu. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
PIRSF	PIRSF000310. NiFe_hyd_ssu. 1 hit.
PRINTS	PR00614. NIHGNASESMLL.
TIGRFAMs	TIGR00391. hydA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHS_WOLSU

Accession

Primary (citable) accession number: P31884

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)