P31883 (MBHL_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Quinone-reactive Ni/Fe-hydrogenase large chain
EC=1.12.5.1

Alternative name(s):
Hydrogen:quinone oxidoreductase
Membrane-bound hydrogenase large subunit

Gene names

Name:	hydB
Ordered Locus Names:	WS1686

Organism

Wolinella succinogenes [Complete proteome] [HAMAP]

Taxonomic identifier

844 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + menaquinone = menaquinol.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogen:quinone oxidoreductase activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 576	575	Quinone-reactive Ni/Fe-hydrogenase large chain		PRO_0000199718
Sites
	Metal binding	62	1	Nickel Potential
	Metal binding	65	1	Nickel Potential
	Metal binding	547	1	Nickel Potential
	Metal binding	550	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P31883 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 091357213B6FF84E	FASTA	576	63,977
	10 20 30 40 50 60 MTKRIVVDPI TRIEGHLRIE VVVDENNVIQ DAFSTATLWR GLETILKGRD PRDAGFFTQR 70 80 90 100 110 120 ICGVCTYSHY KAGISAVENA LGIKPPLNAE LIRSLMSISL ILHDHTVHFY HLHGLDWCDI 130 140 150 160 170 180 TSALKADPVA ASKLAFKYSP NPIATGADEL TAVQKRVAEF AAKGNLGPFA NAYWGHKTYR 190 200 210 220 230 240 FSPEQNLIVL SHYLKALEVQ RVAAQMMAIW GAKQPHPQSL TVGGVTSVMD ALDPSRLGDW 250 260 270 280 290 300 LTKYKYVADF VNRAYYADVV MAAEVFKSEP SVLGGCNVKN FYSYQEIPLN KTEWMYSTGI 310 320 330 340 350 360 VMDGDITKVH EINEDLITEE ATHAWYKENK ALHPYDGQQD PNYTGFKDME TVGPDGTMVK 370 380 390 400 410 420 TKVIDEKGKY TWIKAPRYGG KPLEVGPLAT IVVGLAAKNP RIEKVATQFL KDTGLPLAAL 430 440 450 460 470 480 FTTLGRTAAR MLECKLSADY GFEAFNSLIA NLKVDQSTYT TYKIDKNKEY KGRYMGTVPR 490 500 510 520 530 540 GVLSHWVRIK NGVIQNYQAV VPSTWNAGPR DANGTKGPYE ASLVGMKLQD LSQPLEIIRV 550 560 570 IHSFDPCIAC AVHVMDTKGN ELSQYRVDPI TVGCNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes." Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A. Eur. J. Biochem. 206:93-102(1992) [PubMed: 1587288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11.
[2]	Erratum Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A. Eur. J. Biochem. 214:949-950(1993) [PubMed: 8319698] [Abstract]
[3]	"Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed: 14500908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSMZ 1740.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65189 Genomic DNA. Translation: CAA46303.1. BX571661 Genomic DNA. Translation: CAE10713.1.
PIR	S33853.
RefSeq	NP_907813.1. NC_005090.1.
3D structure databases
ProteinModelPortal	P31883.
ModBase	Search...
Protein family/group databases
TCDB	3.D.7.2.2. H2:heterodisulfide oxidoreductase (HHO) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2554759.
GenomeReviews	Gene locus WS1686 in contig BX571656_GR.
KEGG	wsu:WS1686.
NMPDR	fig|273121.1.peg.1540.
PATRIC	24040330. VBIWolSuc63014_1577.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG733231.
OMA	FKYTSNP.
ProtClustDB	CLSK872401.
Enzyme and pathway databases
BioCyc	WSUC273121:WS1686-MONOMER.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
KO	K05922.
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_WOLSU

Accession

Primary (citable) accession number: P31883

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program