Reviewed,
UniProtKB/Swiss-Prot P31883 (MBHL_WOLSU)
Last modified
June 16, 2009.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Quinone-reactive Ni/Fe-hydrogenase large chain EC=1.12.5.1 Alternative name(s): Membrane-bound hydrogenase large subunit Hydrogen:quinone oxidoreductase | ||||
| Gene names |
| ||||
| Organism | Wolinella succinogenes [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 844 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Wolinella |
Protein attributes
| Sequence length | 576 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions. |
| Catalytic activity | H2 + menaquinone = menaquinol. |
| Cofactor | Binds 1 nickel ion per subunit By similarity. |
| Subunit structure | Heterodimer of a large and a small subunit. |
| Subcellular location | |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Ligand | Metal-binding Nickel |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogen:quinone oxidoreductase activityInferred from electronic annotation. Source: EC nickel ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.1 | ||||||
| Chain | 2 – 576 | 575 | Quinone-reactive Ni/Fe-hydrogenase large chain | PRO_0000199718 | |||||
Sites | |||||||||
| Metal binding | 62 | 1 | Nickel Potential | ||||||
| Metal binding | 65 | 1 | Nickel Potential | ||||||
| Metal binding | 547 | 1 | Nickel Potential | ||||||
| Metal binding | 550 | 1 | Nickel Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes." Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A. Eur. J. Biochem. 206:93-102(1992) [PubMed: 1587288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11. |
| [2] | Erratum Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A. Eur. J. Biochem. 214:949-950(1993) [PubMed: 8319698] [Abstract] |
| [3] | "Complete genome sequence and analysis of Wolinella succinogenes." Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C. Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003) [PubMed: 14500908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSMZ 1740. |
Cross-references
Sequence databases | |
|---|---|
| X65189 Genomic DNA. Translation: CAA46303.1. BX571661 Genomic DNA. Translation: CAE10713.1. | |
| PIR | S33853. |
| RefSeq | NP_907813.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FRF based on UniProtKB P18188. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2554759. |
| GenomeReviews | Gene locus WS1686 in contig BX571656_GR. |
| KEGG | wsu:WS1686. |
| NMPDR | fig|273121.1.peg.1540. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P31883. |
| OMA | P31883. ARCIEAK. |
Enzyme and pathway databases | |
| BioCyc | WSUC273121:WS1686-MON. |
| BRENDA | 1.12.5.1. 191624. |
Family and domain databases | |
| InterPro | IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view] |
| Pfam | PF00374. NiFeSe_Hases. 1 hit. [Graphical view] |
| PROSITE | PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MBHL_WOLSU | ||||||||
| Accession | Primary (citable) accession number: P31883 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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