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P31883 (MBHL_WOLSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Quinone-reactive Ni/Fe-hydrogenase large chain

EC=1.12.5.1
Alternative name(s):
Hydrogen:quinone oxidoreductase
Membrane-bound hydrogenase large subunit
Gene names
Name:hydB
Ordered Locus Names:WS1686
OrganismWolinella succinogenes [Complete proteome] [HAMAP]
Taxonomic identifier844 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeWolinella

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.

Catalytic activity

H2 + menaquinone = menaquinol.

Cofactor

Binds 1 nickel ion per subunit By similarity.

Subunit structure

Heterodimer of a large and a small subunit.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 576575Quinone-reactive Ni/Fe-hydrogenase large chain
PRO_0000199718

Sites

Metal binding621Nickel Potential
Metal binding651Nickel Potential
Metal binding5471Nickel Potential
Metal binding5501Nickel Potential

Sequences

Sequence LengthMass (Da)Tools
P31883 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 091357213B6FF84E

FASTA57663,977
        10         20         30         40         50         60 
MTKRIVVDPI TRIEGHLRIE VVVDENNVIQ DAFSTATLWR GLETILKGRD PRDAGFFTQR 

        70         80         90        100        110        120 
ICGVCTYSHY KAGISAVENA LGIKPPLNAE LIRSLMSISL ILHDHTVHFY HLHGLDWCDI 

       130        140        150        160        170        180 
TSALKADPVA ASKLAFKYSP NPIATGADEL TAVQKRVAEF AAKGNLGPFA NAYWGHKTYR 

       190        200        210        220        230        240 
FSPEQNLIVL SHYLKALEVQ RVAAQMMAIW GAKQPHPQSL TVGGVTSVMD ALDPSRLGDW 

       250        260        270        280        290        300 
LTKYKYVADF VNRAYYADVV MAAEVFKSEP SVLGGCNVKN FYSYQEIPLN KTEWMYSTGI 

       310        320        330        340        350        360 
VMDGDITKVH EINEDLITEE ATHAWYKENK ALHPYDGQQD PNYTGFKDME TVGPDGTMVK 

       370        380        390        400        410        420 
TKVIDEKGKY TWIKAPRYGG KPLEVGPLAT IVVGLAAKNP RIEKVATQFL KDTGLPLAAL 

       430        440        450        460        470        480 
FTTLGRTAAR MLECKLSADY GFEAFNSLIA NLKVDQSTYT TYKIDKNKEY KGRYMGTVPR 

       490        500        510        520        530        540 
GVLSHWVRIK NGVIQNYQAV VPSTWNAGPR DANGTKGPYE ASLVGMKLQD LSQPLEIIRV 

       550        560        570 
IHSFDPCIAC AVHVMDTKGN ELSQYRVDPI TVGCNL 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65189 Genomic DNA. Translation: CAA46303.1.
BX571661 Genomic DNA. Translation: CAE10713.1.
PIRS33853.
RefSeqNP_907813.1. NC_005090.1.

3D structure databases

ProteinModelPortalP31883.
ModBaseSearch...

Protein family/group databases

TCDB3.D.7.2.2. H2:heterodisulfide oxidoreductase (HHO) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2554759.
GenomeReviewsGene locus WS1686 in contig BX571656_GR.
KEGGwsu:WS1686.
NMPDRfig|273121.1.peg.1540.
PATRIC24040330. VBIWolSuc63014_1577.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG733231.
OMAFKYTSNP.
ProtClustDBCLSK872401.

Enzyme and pathway databases

BioCycWSUC273121:WS1686-MONOMER.

Family and domain databases

InterProIPR001501. Ni-dep_hyd_lsu.
IPR018194. Ni-dep_hyd_lsu_Ni_BS.
[Graphical view]
KOK05922.
PfamPF00374. NiFeSe_Hases. 1 hit.
[Graphical view]
PROSITEPS00507. NI_HGENASE_L_1. 1 hit.
PS00508. NI_HGENASE_L_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMBHL_WOLSU
AccessionPrimary (citable) accession number: P31883
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families