Reviewed, UniProtKB/Swiss-Prot P31865 (KPYK_TRIRE)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:

pki1

Organism

Trichoderma reesei (Hypocrea jecorina)

Taxonomic identifier

51453 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Pyruvate kinase		PRO_0000112119
Sites
	Active site	263	1	By similarity
	Metal binding	265	1	Magnesium Potential
	Metal binding	286	1	Magnesium Potential
	Metal binding	287	1	Magnesium Potential
	Binding site	360	1	ADP Potential
Amino acid modifications
	Modified residue	45	1	Phosphoserine Potential

Sequences

	Sequence		Length	Mass (Da)
	P31865-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: E9C26AC0E8186FE7	FASTA	538	58,888
	10 20 30 40 50 60 MSQISRTQSI MATTAQEHLE TGGRINWLAS LNTAFTPARN FRRTSIICTI GPKTNSVEAL 70 80 90 100 110 120 NKLRDAGLNV ARMNFSHGSY EYHQSVIDNV RASVAAHPGR PVAIALDTKG PEIRTGNTAG 130 140 150 160 170 180 DVDIPISAGT VMNFTTDEKY ATACDTQNMY VDYKNITKVI QPGRVIYVDD GVLAFDVLSI 190 200 210 220 230 240 KDDQTVEVRA RNNGFISSRK GVNLPNTDVD LPALSEKDKA DLRFGVKNNV DMVFASFIRR 250 260 270 280 290 300 AQDIKDIRDV LGPEGKQIQI IAKIENRQGL NNFAEILEET DGVMVARGDL GIEIPAAEVF 310 320 330 340 350 360 AAQKKMIAMC NIAGKPVICA TQMLESMIKN PRPTRAEISD VGNAVTDGAD CVMLSGETAK 370 380 390 400 410 420 GNYPAESIHE MHEASLKAEN TIPYVSHFEE MCTLVKRPVS TVESCAMAAV RASLDLGAGG 430 440 450 460 470 480 IIVLSTSGDS ARLLSKYRPV CPIFMVTRNP TTSRFSHLYR GVYPFLYPEQ KPDFDTVNWQ 490 500 510 520 530 EDVDKRIKWA VTRAIELKTL TAGDTVVVVQ GWKGGMGNTN TLRIVRADPD HLGIGQME

References

[1]	"Characterization of the pyruvate kinase-encoding gene (pki1) of Trichoderma reesei." Schindler M., Mach R.L., Vollenhofer S.K., Hodits R., Gruber F., Visser J., de Graaff L., Kubicek C.P. Gene 130:271-275(1993) [PubMed: 8359694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07060 Unassigned DNA. Translation: AAA02922.1.
PIR	JN0780.
3D structure databases
HSSP	HSSP built from PDB template 1A3W based on UniProtKB P00549.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 280374.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_TRIRE

Accession

Primary (citable) accession number: P31865

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)