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Protein

Pyruvate kinase

Gene

pki1

Organism
Hypocrea jecorina (Trichoderma reesei)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  2. Phosphoglycerate kinase (pgk1)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase (pki1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72SubstrateBy similarity1
Metal bindingi74PotassiumBy similarity1
Metal bindingi76PotassiumBy similarity1
Metal bindingi107PotassiumBy similarity1
Metal bindingi108Potassium; via carbonyl oxygenBy similarity1
Sitei263Transition state stabilizerBy similarity1
Metal bindingi265MagnesiumBy similarity1
Binding sitei288Substrate; via amide nitrogenBy similarity1
Metal bindingi289MagnesiumBy similarity1
Binding sitei289Substrate; via amide nitrogenBy similarity1
Binding sitei321SubstrateBy similarity1
Binding sitei360ADPSequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pki1
OrganismiHypocrea jecorina (Trichoderma reesei)
Taxonomic identifieri51453 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121191 – 538Pyruvate kinaseAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45PhosphoserineSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP31865.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi51453.JGI78439.

Structurei

3D structure databases

ProteinModelPortaliP31865.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
OMAiTHEDHKE.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQISRTQSI MATTAQEHLE TGGRINWLAS LNTAFTPARN FRRTSIICTI
60 70 80 90 100
GPKTNSVEAL NKLRDAGLNV ARMNFSHGSY EYHQSVIDNV RASVAAHPGR
110 120 130 140 150
PVAIALDTKG PEIRTGNTAG DVDIPISAGT VMNFTTDEKY ATACDTQNMY
160 170 180 190 200
VDYKNITKVI QPGRVIYVDD GVLAFDVLSI KDDQTVEVRA RNNGFISSRK
210 220 230 240 250
GVNLPNTDVD LPALSEKDKA DLRFGVKNNV DMVFASFIRR AQDIKDIRDV
260 270 280 290 300
LGPEGKQIQI IAKIENRQGL NNFAEILEET DGVMVARGDL GIEIPAAEVF
310 320 330 340 350
AAQKKMIAMC NIAGKPVICA TQMLESMIKN PRPTRAEISD VGNAVTDGAD
360 370 380 390 400
CVMLSGETAK GNYPAESIHE MHEASLKAEN TIPYVSHFEE MCTLVKRPVS
410 420 430 440 450
TVESCAMAAV RASLDLGAGG IIVLSTSGDS ARLLSKYRPV CPIFMVTRNP
460 470 480 490 500
TTSRFSHLYR GVYPFLYPEQ KPDFDTVNWQ EDVDKRIKWA VTRAIELKTL
510 520 530
TAGDTVVVVQ GWKGGMGNTN TLRIVRADPD HLGIGQME
Length:538
Mass (Da):58,888
Last modified:July 1, 1993 - v1
Checksum:iE9C26AC0E8186FE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07060 Unassigned DNA. Translation: AAA02922.1.
PIRiJN0780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07060 Unassigned DNA. Translation: AAA02922.1.
PIRiJN0780.

3D structure databases

ProteinModelPortaliP31865.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi51453.JGI78439.

Proteomic databases

PRIDEiP31865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
OMAiTHEDHKE.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_HYPJE
AccessioniPrimary (citable) accession number: P31865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 5, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.