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Protein

Pyruvate kinase

Gene

pki1

Organism
Hypocrea jecorina (Trichoderma reesei)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721SubstrateBy similarity
Metal bindingi74 – 741PotassiumBy similarity
Metal bindingi76 – 761PotassiumBy similarity
Metal bindingi107 – 1071PotassiumBy similarity
Metal bindingi108 – 1081Potassium; via carbonyl oxygenBy similarity
Sitei263 – 2631Transition state stabilizerBy similarity
Metal bindingi265 – 2651MagnesiumBy similarity
Binding sitei288 – 2881Substrate; via amide nitrogenBy similarity
Metal bindingi289 – 2891MagnesiumBy similarity
Binding sitei289 – 2891Substrate; via amide nitrogenBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Binding sitei360 – 3601ADPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pki1
OrganismiHypocrea jecorina (Trichoderma reesei)
Taxonomic identifieri51453 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesHypocreaceaeTrichoderma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Pyruvate kinasePRO_0000112119Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei45 – 451PhosphoserineSequence Analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP31865.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi51453.JGI78439.

Structurei

3D structure databases

ProteinModelPortaliP31865.
SMRiP31865. Positions 35-524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
OMAiDRVMKSR.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31865-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQISRTQSI MATTAQEHLE TGGRINWLAS LNTAFTPARN FRRTSIICTI
60 70 80 90 100
GPKTNSVEAL NKLRDAGLNV ARMNFSHGSY EYHQSVIDNV RASVAAHPGR
110 120 130 140 150
PVAIALDTKG PEIRTGNTAG DVDIPISAGT VMNFTTDEKY ATACDTQNMY
160 170 180 190 200
VDYKNITKVI QPGRVIYVDD GVLAFDVLSI KDDQTVEVRA RNNGFISSRK
210 220 230 240 250
GVNLPNTDVD LPALSEKDKA DLRFGVKNNV DMVFASFIRR AQDIKDIRDV
260 270 280 290 300
LGPEGKQIQI IAKIENRQGL NNFAEILEET DGVMVARGDL GIEIPAAEVF
310 320 330 340 350
AAQKKMIAMC NIAGKPVICA TQMLESMIKN PRPTRAEISD VGNAVTDGAD
360 370 380 390 400
CVMLSGETAK GNYPAESIHE MHEASLKAEN TIPYVSHFEE MCTLVKRPVS
410 420 430 440 450
TVESCAMAAV RASLDLGAGG IIVLSTSGDS ARLLSKYRPV CPIFMVTRNP
460 470 480 490 500
TTSRFSHLYR GVYPFLYPEQ KPDFDTVNWQ EDVDKRIKWA VTRAIELKTL
510 520 530
TAGDTVVVVQ GWKGGMGNTN TLRIVRADPD HLGIGQME
Length:538
Mass (Da):58,888
Last modified:July 1, 1993 - v1
Checksum:iE9C26AC0E8186FE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07060 Unassigned DNA. Translation: AAA02922.1.
PIRiJN0780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07060 Unassigned DNA. Translation: AAA02922.1.
PIRiJN0780.

3D structure databases

ProteinModelPortaliP31865.
SMRiP31865. Positions 35-524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi51453.JGI78439.

Proteomic databases

PRIDEiP31865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG0469.
OMAiDRVMKSR.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the pyruvate kinase-encoding gene (pki1) of Trichoderma reesei."
    Schindler M., Mach R.L., Vollenhofer S.K., Hodits R., Gruber F., Visser J., de Graaff L., Kubicek C.P.
    Gene 130:271-275(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKPYK_HYPJE
AccessioniPrimary (citable) accession number: P31865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: January 7, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.