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Protein

Ligninase B

Gene

LIPB

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.

Catalytic activityi

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H2O.
2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4-dimethoxyphenyl)methanol radical + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Pathwayi: lignin degradation

This protein is involved in the pathway lignin degradation, which is part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the pathway lignin degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei71 – 711Transition state stabilizerPROSITE-ProRule annotation
Active sitei75 – 751Proton acceptorPROSITE-ProRule annotation
Metal bindingi76 – 761Calcium 1PROSITE-ProRule annotation
Metal bindingi94 – 941Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi96 – 961Calcium 1PROSITE-ProRule annotation
Metal bindingi98 – 981Calcium 1PROSITE-ProRule annotation
Metal bindingi204 – 2041Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi205 – 2051Calcium 2PROSITE-ProRule annotation
Metal bindingi222 – 2221Calcium 2PROSITE-ProRule annotation
Metal bindingi224 – 2241Calcium 2PROSITE-ProRule annotation
Metal bindingi227 – 2271Calcium 2; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi229 – 2291Calcium 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Lignin degradation

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00892.

Names & Taxonomyi

Protein namesi
Recommended name:
Ligninase B (EC:1.11.1.14)
Alternative name(s):
Diarylpropane peroxidase
Lignin peroxidase
Gene namesi
Name:LIPB
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 287Sequence analysisPRO_0000023774
Chaini29 – 372344Ligninase BPRO_0000023775Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 43PROSITE-ProRule annotation
Disulfide bondi42 ↔ 313PROSITE-ProRule annotation
Disulfide bondi62 ↔ 148PROSITE-ProRule annotation
Disulfide bondi277 ↔ 345PROSITE-ProRule annotation
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP31838.
SMRiP31838. Positions 24-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Ligninase subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II1B. Eukaryota.
ENOG410ZS1R. LUCA.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR024589. Ligninase_C.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF11895. DUF3415. 1 hit.
PF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00462. LIGNINASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFKQLFAAI SLALSLSAAN AAAVIEKRAT CSNGKTVGDA SCCAWFDVLD
60 70 80 90 100
DIQQNLFHGG QCGAEAHESI RLVFHDSIAI SPAMEAQGKF GGGGADGSIM
110 120 130 140 150
IFDDIETAFH PNIGLDEIVK LQKPFVQKHG VTPGAFIAFA GAVALSNCPG
160 170 180 190 200
APQMNFFTGR APATQPAPDG LVPEPFHTVD QIINRVNDAG EFDELELVWM
210 220 230 240 250
LSAHSVAAVN DVDPTVQGLP FDSTPGIFDS QFFVETQLRG TAFPGSGGNQ
260 270 280 290 300
GEVESPLPGE IRIQSDHTIA RDSRTACEWQ SFVNNQSKLV DDFQFIFLAL
310 320 330 340 350
TQLGQDPNAM TDCSDVIPQS KPIPGNLPFS FFPAGKTIKD VEQACAETPF
360 370
PTLTTLPGPE TSVQRIPPPP GA
Length:372
Mass (Da):39,530
Last modified:July 1, 1993 - v1
Checksum:iE0DD2CF40425D9D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54257 Genomic DNA. Translation: CAA38178.1.
PIRiA32322.
B32322.
PS0010.
S13564. OPJGBP.
S69246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54257 Genomic DNA. Translation: CAA38178.1.
PIRiA32322.
B32322.
PS0010.
S13564. OPJGBP.
S69246.

3D structure databases

ProteinModelPortaliP31838.
SMRiP31838. Positions 24-372.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410II1B. Eukaryota.
ENOG410ZS1R. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00892.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR024589. Ligninase_C.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF11895. DUF3415. 1 hit.
PF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00462. LIGNINASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium."
    Gaskell J., Dieperink E., Cullen D.
    Nucleic Acids Res. 19:599-603(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24725 / DSM 6909 / CBS 481.73 / BCRC 36200 / NRRL 6361 / VKM F-1767.

Entry informationi

Entry nameiLIGB_PHACH
AccessioniPrimary (citable) accession number: P31838
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.