Reviewed, UniProtKB/Swiss-Prot P31835 (CDGT2_PAEMA)

Names and origin

Protein names	Recommended name:     Cyclomaltodextrin glucanotransferase     EC=2.4.1.19 Alternative name(s):     Cyclodextrin-glycosyltransferase       Short name=CGTase
Organism	Paenibacillus macerans (Bacillus macerans)
Taxonomic identifier	44252 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Secreted By similarity.
Domain	May consist of two protein domains: the one in the N-terminal side cleaves the alpha-1,4-glucosidic bond in starch, and the other in the C-terminal side catalyzes other activities, including the reconstitution of an alpha-1,4-glucosidic linkage for cyclizing the maltooligosaccharide produced.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. Contains 1 CBM20 (carbohydrate binding type-20) domain. Contains 1 IPT/TIG domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro cyclomaltodextrin glucanotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.1
	Chain	28 – 713	686	Cyclomaltodextrin glucanotransferase		PRO_0000001435
Regions
	Domain	526 – 606	81	IPT/TIG
	Domain	608 – 713	106	CBM20
	Region	28 – 165	138	A1
	Region	166 – 229	64	B
	Region	230 – 434	205	A2
	Region	435 – 522	88	C
	Region	523 – 609	87	D
	Region	610 – 713	104	E
Sites
	Active site	256	1	By similarity
	Active site	285	1	By similarity
	Active site	356	1	By similarity
	Metal binding	54	1	Calcium 2 By similarity
	Metal binding	56	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	59	1	Calcium 2 By similarity
	Metal binding	60	1	Calcium 2 By similarity
	Metal binding	78	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium 2 By similarity
	Metal binding	166	1	Calcium 1 By similarity
	Metal binding	217	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	226	1	Calcium 1 By similarity
	Metal binding	260	1	Calcium 1; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31835-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 5A287BCC4AAFE635	FASTA	713	76,857
	10 20 30 40 50 60 MKKQVKWLTS VSMSVGIALG AALPVWASPD TSVNNKLNFS TDTVYQIVTD RFVDGNSANN 70 80 90 100 110 120 PTGAAFSSDH SNLKLYFGGD WQGITNKIND GYLTGMGITA LWISQPVENI TAVINYSGVN 130 140 150 160 170 180 NTAYHGYWPR DFKKTNAAFG SFTDFSNLIA AAHSHNIKVV MDFAPNHTNP ASSTDPSFAE 190 200 210 220 230 240 NGALYNNGTL LGKYSNDTAG LFHHNGGTDF STTESGIYKN LYDLADINQN NNTIDSYLKE 250 260 270 280 290 300 SIQLWLNLGV DGIRFDAVKH MPQGWQKSYV SSIYSSANPV FTFGEWFLGP DEMTQDNINF 310 320 330 340 350 360 ANQSGMHLLD FAFAQEIREV FRDKSETMTD LNSVISSTGS SYNYINNMVT FIDNHDMDRF 370 380 390 400 410 420 QQAGASTRPT EQALAVTLTS RGVPAIYYGT EQYMTGNGDP NNRGMMTGFD TNKTAYKVIK 430 440 450 460 470 480 ALAPLRKSNP ALAYGSTTQR WVNSDVYVYE RKFGSNVALV AVNRSSTTAY PISGALTALP 490 500 510 520 530 540 NGTYTDVLGG LLNGNSITVN GGTVSNFTLA AGGTAVWQYT TTESSPIIGN VGPTMGKPGN 550 560 570 580 590 600 TITIDGRGFG TTKNKVTFGT TAVTGANIVS WEDTEIKVKV PNVAAGNTAV TVTNAAGTTS 610 620 630 640 650 660 AAFNNFNVLT ADQVTVRFKV NNATTALGQN VYLTGNVAEL GNWTAANAIG PMYNQVEASY 670 680 690 700 710 PTWYFDVSVP ANTALQFKFI KVNGSTVTWE GGNNHTFTSP SSGVATVTVD WQN

References

[1]	"Polypeptide possessing cyclomaltodextrin glucanotransferase activity." Sugimoto T., Kubota M., Sakai S. Patent number GB2169902, 23-JUL-1986 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-37.

Cross-references

Sequence databases
PIR	ALBSXR. S26589.
3D structure databases
HSSP	HSSP built from PDB template 1CXL based on UniProtKB P43379.
ModBase	Search...
Protein family/group databases
CAZy	CBM20. Carbohydrate-Binding Module Family 20. GH13. Glycoside Hydrolase Family 13.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR002044. Glyco_hydro_carb-bd. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR002909. IPT_TIG_rcpt. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF00686. CBM_20. 1 hit. PF01833. TIG. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
ProDom	PD001568. Glyco_hydro_CBD. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
PROSITE	PS51166. CBM20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDGT2_PAEMA

Accession

Primary (citable) accession number: P31835

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)