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Protein

tRNA1(Val) (adenine(37)-N6)-methyltransferase

Gene

yfiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the adenine in position 37 of tRNA1(Val) (anticodon cmo5UAC).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + adenine(37) in tRNA1(Val) = S-adenosyl-L-homocysteine + N(6)-methyladenine(37) in tRNA1(Val).1 Publication

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • tRNA (adenine-N6-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • tRNA methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11538-MONOMER.
ECOL316407:JW2559-MONOMER.
MetaCyc:EG11538-MONOMER.
BRENDAi2.1.1.223. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA1(Val) (adenine(37)-N6)-methyltransferase (EC:2.1.1.223)
Alternative name(s):
tRNA m6A37 methyltransferase
Gene namesi
Name:yfiC
Ordered Locus Names:b2575, JW2559
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11538. yfiC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show hypersensitivity to hyperosmotic and, to a lesser extent, oxidative stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245tRNA1(Val) (adenine(37)-N6)-methyltransferasePRO_0000169266Add
BLAST

Proteomic databases

PaxDbiP31825.
PRIDEiP31825.

Interactioni

Protein-protein interaction databases

BioGridi4260913. 18 interactions.
IntActiP31825. 4 interactions.
STRINGi511145.b2575.

Structurei

3D structure databases

ProteinModelPortaliP31825.
SMRiP31825. Positions 44-135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107S47. Bacteria.
COG4123. LUCA.
HOGENOMiHOG000283147.
InParanoidiP31825.
KOiK15460.
OMAiDRLFCYH.
OrthoDBiEOG66TGB2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01872. tRNA_methyltr_YfiC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR022882. tRNA_adenine-N6_MeTrfase.
[Graphical view]
PfamiPF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSTSVLRR NGFTFKQFFV AHDRCAMKVG TDGILLGAWA PVAGVKRCLD
60 70 80 90 100
IGAGSGLLAL MLAQRTDDSV MIDAVELESE AAAQAQENIN QSPWAERINV
110 120 130 140 150
HTADIQQWIT QQTVRFDLII SNPPYYQQGV ECSTPQREQA RYTTTLDHPS
160 170 180 190 200
LLTCAAECIT EEGFFCVVLP EQIGNGFTEL ALSMGWHLRL RTDVAENEAR
210 220 230 240
LPHRVLLAFS PQAGECFSDR LVIRGPDQNY SEAYTALTQA FYLFM
Length:245
Mass (Da):27,270
Last modified:April 27, 2001 - v2
Checksum:iCEB4F1E3A849B7E3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831A → R in D13169 (Ref. 1) Curated
Sequence conflicti83 – 831A → R in D64044 (Ref. 2) Curated
Sequence conflicti211 – 24535PQAGE…FYLFM → RRRENALAIA in D13169 (Ref. 1) CuratedAdd
BLAST
Sequence conflicti211 – 24535PQAGE…FYLFM → RRRENALAIA in D64044 (Ref. 2) CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA. No translation available.
D64044 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75628.2.
AP009048 Genomic DNA. Translation: BAE76751.1.
PIRiF65035.
RefSeqiNP_417070.2. NC_000913.3.
WP_001295363.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75628; AAC75628; b2575.
BAE76751; BAE76751; BAE76751.
GeneIDi947047.
KEGGiecj:JW2559.
eco:b2575.
PATRICi32120549. VBIEscCol129921_2677.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA. No translation available.
D64044 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75628.2.
AP009048 Genomic DNA. Translation: BAE76751.1.
PIRiF65035.
RefSeqiNP_417070.2. NC_000913.3.
WP_001295363.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31825.
SMRiP31825. Positions 44-135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260913. 18 interactions.
IntActiP31825. 4 interactions.
STRINGi511145.b2575.

Proteomic databases

PaxDbiP31825.
PRIDEiP31825.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75628; AAC75628; b2575.
BAE76751; BAE76751; BAE76751.
GeneIDi947047.
KEGGiecj:JW2559.
eco:b2575.
PATRICi32120549. VBIEscCol129921_2677.

Organism-specific databases

EchoBASEiEB1500.
EcoGeneiEG11538. yfiC.

Phylogenomic databases

eggNOGiENOG4107S47. Bacteria.
COG4123. LUCA.
HOGENOMiHOG000283147.
InParanoidiP31825.
KOiK15460.
OMAiDRLFCYH.
OrthoDBiEOG66TGB2.

Enzyme and pathway databases

BioCyciEcoCyc:EG11538-MONOMER.
ECOL316407:JW2559-MONOMER.
MetaCyc:EG11538-MONOMER.
BRENDAi2.1.1.223. 2026.

Miscellaneous databases

PROiP31825.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01872. tRNA_methyltr_YfiC.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
IPR007848. Small_mtfrase_dom.
IPR022882. tRNA_adenine-N6_MeTrfase.
[Graphical view]
PfamiPF05175. MTS. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Non-ribosomal proteins affecting the assembly of ribosomes in Escherichia coli."
    Nashimoto H.
    (In) Nierhaus K.H. (eds.); The translational apparatus, pp.185-195, Plenum Press, New York (1993)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Nashimoto H., Saito N.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Baum B., Koonin E.V.
    Unpublished observations (MAY-1993)
    Cited for: SIMILARITY TO METHYLTRANSFERASES.
  6. "The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that specifically modifies A37 of tRNA1Val(cmo5UAC)."
    Golovina A.Y., Sergiev P.V., Golovin A.V., Serebryakova M.V., Demina I., Govorun V.M., Dontsova O.A.
    RNA 15:1134-1141(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A METHYLTRANSFERASE, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    Strain: BW25141.

Entry informationi

Entry nameiTRMN6_ECOLI
AccessioniPrimary (citable) accession number: P31825
Secondary accession number(s): P76591, Q2MAF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: May 11, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.