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Protein

tRNA1(Val) (adenine(37)-N6)-methyltransferase

Gene

yfiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the adenine in position 37 of tRNA1(Val) (anticodon cmo5UAC).1 Publication

Catalytic activityi

S-adenosyl-L-methionine + adenine(37) in tRNA1(Val) = S-adenosyl-L-homocysteine + N6-methyladenine(37) in tRNA1(Val).1 Publication

GO - Molecular functioni

  • nucleic acid binding Source: InterPro
  • tRNA (adenine-N6-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • tRNA methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11538-MONOMER
MetaCyc:EG11538-MONOMER
BRENDAi2.1.1.223 2026

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA1(Val) (adenine(37)-N6)-methyltransferase (EC:2.1.1.223)
Alternative name(s):
tRNA m6A37 methyltransferase
Gene namesi
Name:yfiC
Ordered Locus Names:b2575, JW2559
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11538 yfiC

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show hypersensitivity to hyperosmotic and, to a lesser extent, oxidative stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001692661 – 245tRNA1(Val) (adenine(37)-N6)-methyltransferaseAdd BLAST245

Proteomic databases

PaxDbiP31825
PRIDEiP31825

Interactioni

Protein-protein interaction databases

BioGridi4260913, 33 interactors
IntActiP31825, 4 interactors
STRINGi316385.ECDH10B_2743

Structurei

3D structure databases

ProteinModelPortaliP31825
SMRiP31825
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107S47 Bacteria
COG4123 LUCA
HOGENOMiHOG000283147
InParanoidiP31825
KOiK15460
OMAiDKCAMKV

Family and domain databases

HAMAPiMF_01872 tRNA_methyltr_YfiC, 1 hit
InterProiView protein in InterPro
IPR002052 DNA_methylase_N6_adenine_CS
IPR029063 SAM-dependent_MTases
IPR007848 Small_mtfrase_dom
IPR022882 tRNA_adenine-N6_MeTrfase
PfamiView protein in Pfam
PF05175 MTS, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P31825-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSTSVLRR NGFTFKQFFV AHDRCAMKVG TDGILLGAWA PVAGVKRCLD
60 70 80 90 100
IGAGSGLLAL MLAQRTDDSV MIDAVELESE AAAQAQENIN QSPWAERINV
110 120 130 140 150
HTADIQQWIT QQTVRFDLII SNPPYYQQGV ECSTPQREQA RYTTTLDHPS
160 170 180 190 200
LLTCAAECIT EEGFFCVVLP EQIGNGFTEL ALSMGWHLRL RTDVAENEAR
210 220 230 240
LPHRVLLAFS PQAGECFSDR LVIRGPDQNY SEAYTALTQA FYLFM
Length:245
Mass (Da):27,270
Last modified:April 27, 2001 - v2
Checksum:iCEB4F1E3A849B7E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83A → R in D13169 (Ref. 1) Curated1
Sequence conflicti83A → R in D64044 (Ref. 2) Curated1
Sequence conflicti211 – 245PQAGE…FYLFM → RRRENALAIA in D13169 (Ref. 1) CuratedAdd BLAST35
Sequence conflicti211 – 245PQAGE…FYLFM → RRRENALAIA in D64044 (Ref. 2) CuratedAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA No translation available.
D64044 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC75628.2
AP009048 Genomic DNA Translation: BAE76751.1
PIRiF65035
RefSeqiNP_417070.2, NC_000913.3
WP_001295363.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75628; AAC75628; b2575
BAE76751; BAE76751; BAE76751
GeneIDi947047
KEGGiecj:JW2559
eco:b2575
PATRICifig|511145.12.peg.2677

Similar proteinsi

Entry informationi

Entry nameiTRMN6_ECOLI
AccessioniPrimary (citable) accession number: P31825
Secondary accession number(s): P76591, Q2MAF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: March 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health