Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P31822

- POL_FIVT2

UniProt

P31822 - POL_FIVT2

Protein

Pol polyprotein

Gene

pol

Organism
Feline immunodeficiency virus (isolate TM2) (FIV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

    Catalytic activityi

    Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
    3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
    dUTP + H2O = dUMP + diphosphate.
    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei67 – 671For protease activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri848 – 88942Integrase-typePROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi1067 – 111549Integrase-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. dUTP diphosphatase activity Source: UniProtKB-EC
    4. exoribonuclease H activity Source: UniProtKB-EC
    5. RNA binding Source: InterPro
    6. RNA-directed DNA polymerase activity Source: UniProtKB-KW
    7. RNA-DNA hybrid ribonuclease activity Source: InterPro
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW
    2. DNA recombination Source: UniProtKB-KW
    3. dUTP metabolic process Source: InterPro
    4. establishment of integrated proviral latency Source: UniProtKB-KW
    5. viral entry into host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

    Keywords - Biological processi

    DNA integration, DNA recombination, Nucleotide metabolism, Viral genome integration, Virus entry into host cell

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiA02.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pol polyprotein
    Cleaved into the following 4 chains:
    Alternative name(s):
    Retropepsin
    Alternative name(s):
    Exoribonuclease H (EC:3.1.13.2)
    Integrase
    Short name:
    IN
    Gene namesi
    Name:pol
    OrganismiFeline immunodeficiency virus (isolate TM2) (FIV)
    Taxonomic identifieri31676 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusFeline lentivirus group
    Virus hostiFelidae (cat family) [TaxID: 9681]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 153153ProteaseBy similarityPRO_0000038849Add
    BLAST
    Chaini154 – 709556Reverse transcriptase/ribonuclease HBy similarityPRO_0000038850Add
    BLAST
    Chaini710 – 843134Deoxyuridine 5'-triphosphate nucleotidohydrolaseBy similarityPRO_0000038851Add
    BLAST
    Chaini844 – 1124281IntegraseBy similarityPRO_0000038852Add
    BLAST

    Post-translational modificationi

    Cleavage sites that yield the mature proteins remain to be determined.

    Structurei

    3D structure databases

    ProteinModelPortaliP31822.
    SMRiP31822. Positions 41-153, 710-843.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini62 – 14382Peptidase A2PROSITE-ProRule annotationAdd
    BLAST
    Domaini199 – 388190Reverse transcriptasePROSITE-ProRule annotationAdd
    BLAST
    Domaini591 – 711121RNase HPROSITE-ProRule annotationAdd
    BLAST
    Domaini899 – 1049151Integrase catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the retroviral Pol polyprotein family.Curated
    Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
    Contains 1 integrase-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 integrase-type zinc finger.PROSITE-ProRule annotation
    Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
    Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
    Contains 1 RNase H domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri848 – 88942Integrase-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    Gene3Di1.10.10.200. 1 hit.
    2.30.30.10. 1 hit.
    2.40.70.10. 1 hit.
    2.70.40.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProiIPR001969. Aspartic_peptidase_AS.
    IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR001037. Integrase_C_retrovir.
    IPR001584. Integrase_cat-core.
    IPR017856. Integrase_Zn-bd_dom-like_N.
    IPR003308. Integrase_Zn-bd_dom_N.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    IPR010659. RVT_connect.
    IPR010661. RVT_thumb.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    PF00552. IN_DBD_C. 1 hit.
    PF02022. Integrase_Zn. 1 hit.
    PF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    PF06815. RVT_connect. 1 hit.
    PF06817. RVT_thumb. 1 hit.
    [Graphical view]
    SUPFAMiSSF46919. SSF46919. 1 hit.
    SSF50122. SSF50122. 1 hit.
    SSF50630. SSF50630. 1 hit.
    SSF51283. SSF51283. 1 hit.
    SSF53098. SSF53098. 2 hits.
    PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS51027. INTEGRASE_DBD. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    PS50876. ZF_INTEGRASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31822-1 [UniParc]FASTAAdd to Basket

    « Hide

    ENLGKREGGA SCSPSKPSAA NSTICTSNGG ETIRFINYNT IGTTTTLERR     50
    PEIQIFVNGH PIKFLLDTGA DITILNRKDF QIGNSIENGK QNMIGVGGGK 100
    RGTNYINVHL EIRDENYRMQ CIFGNVCVLE DNSLIQPLLG RDNMIKFNIR 150
    LVMAQISEKI PIVKVRMKDP TQGPQVKQWP LSNEKIEALT DIVERLESEG 200
    KVKRADPNNP WNTPVFAIKK KSGKWRMLID FRVLNKLTDK GAEVQLGLPH 250
    PAGLQMKKQV TVLDIGDAYF TIPLDPDYAP YTAFTLPRKN NAGPGRRYVW 300
    CSLPQGWVLS PLIYQSTLNN ILQPFIKQNS ELDIYQYMDD IYIGSNLNKK 350
    EHKQKVEELR KLLLWWGFET PEDKLQEEPP YKWMGYELHP LTWSIQQKQL 400
    EIPERPTLNE LQKLAGKINW ASQTIPDLSI KELTNMMRGD QKLDSIREWT 450
    VEAKREVQKA KEAIETQAQL NYYDPNRGLY AKLSLVGPHQ ICYQVYQKNP 500
    EHILWYGKIN RQKKKAENTC DIALRACYKI REESIIRIGK EPVYEIPASR 550
    EAWESNLIRS PYLKAPPPEV EFIHAALSIK RALSMIQDAP IIGAETWYID 600
    GSRKQGKAAR AAYWTNTGKW QIMEIEGSNQ KAEVQALLLA LKAGSEEMNI 650
    ITDSQYILNI LNQQPDLMEG LWQEVLEQME KKIAIFIDWV PGHKGIPGNE 700
    EVDKLCQTMM IIEGEGILEK RSEDAGYDLL AAAQETHFLP GEVRIVPTKT 750
    RIMLPKGHWG LIMGKSSIGS KGVDVLGGVI DEGYRGELGV IMINLTKKSI 800
    TILEKQKIAQ LIILPCRHEG LQQGEIQMNS ERGEKGFGSA GVFSSWVDRI 850
    EEAELNHEKF HSDPQYLRTE FNLPRIVAEE IKRKCPLCRI RGEQVGGQLK 900
    IRPGIWQMDC THFNGKIIIV AVHVESGFLW AQIIPQETAD CTVKALLQLI 950
    CAHNVTELQT DNGPNFKNQK MEGLLNYMGI KHKLGIPGNP QSQALVENAN 1000
    NTLKVWIQKF LPETTSLDNA LALALHCLNF KQRGRLGRMA PYELYIQQES 1050
    LRIQDYFSAI PQKLMMQWLY YKDQKDKKWK GPMRVEYWGQ GSVLLKDEEK 1100
    GIFLVPRRHI RRVPEPCTLP EGDE 1124
    Length:1,124
    Mass (Da):128,101
    Last modified:July 1, 1993 - v1
    Checksum:iF287FE90FC978296
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59418 Genomic RNA. Translation: AAA43071.1.
    PIRiB45557.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59418 Genomic RNA. Translation: AAA43071.1 .
    PIRi B45557.

    3D structure databases

    ProteinModelPortali P31822.
    SMRi P31822. Positions 41-153, 710-843.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi A02.007.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.10.200. 1 hit.
    2.30.30.10. 1 hit.
    2.40.70.10. 1 hit.
    2.70.40.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProi IPR001969. Aspartic_peptidase_AS.
    IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR001037. Integrase_C_retrovir.
    IPR001584. Integrase_cat-core.
    IPR017856. Integrase_Zn-bd_dom-like_N.
    IPR003308. Integrase_Zn-bd_dom_N.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    IPR010659. RVT_connect.
    IPR010661. RVT_thumb.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    PF00552. IN_DBD_C. 1 hit.
    PF02022. Integrase_Zn. 1 hit.
    PF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    PF06815. RVT_connect. 1 hit.
    PF06817. RVT_thumb. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46919. SSF46919. 1 hit.
    SSF50122. SSF50122. 1 hit.
    SSF50630. SSF50630. 1 hit.
    SSF51283. SSF51283. 1 hit.
    SSF53098. SSF53098. 2 hits.
    PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS51027. INTEGRASE_DBD. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    PS50876. ZF_INTEGRASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of feline immunodeficiency virus rev gene activity."
      Kiyomasu T., Miyazawa T., Furuya T., Shibata R., Sakai H., Sakuragi J.I., Fukasawa M., Maki N., Hasegawa A., Mikami T., Adachi A.
      J. Virol. 65:4539-4542(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Molecular characterization and heterogeneity of feline immunodeficiency virus isolates."
      Maki N., Miyazawa T., Fukasawa M., Hasegawa A., Hayami M., Miki K., Mikami T.
      Arch. Virol. 123:29-45(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiPOL_FIVT2
    AccessioniPrimary (citable) accession number: P31822
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3