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P31822

- POL_FIVT2

UniProt

P31822 - POL_FIVT2

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Protein

Pol polyprotein

Gene

pol

Organism
Feline immunodeficiency virus (isolate TM2) (FIV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
dUTP + H2O = dUMP + diphosphate.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671For protease activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri848 – 88942Integrase-typePROSITE-ProRule annotationAdd
BLAST
DNA bindingi1067 – 111549Integrase-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. dUTP diphosphatase activity Source: UniProtKB-EC
  4. exoribonuclease H activity Source: UniProtKB-EC
  5. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  6. RNA-DNA hybrid ribonuclease activity Source: InterPro
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. DNA recombination Source: UniProtKB-KW
  3. dUTP metabolic process Source: InterPro
  4. establishment of integrated proviral latency Source: UniProtKB-KW
  5. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Nucleotide metabolism, Viral genome integration, Virus entry into host cell

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Protein family/group databases

MEROPSiA02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 4 chains:
Alternative name(s):
Retropepsin
Alternative name(s):
Exoribonuclease H (EC:3.1.13.2)
Integrase
Short name:
IN
Gene namesi
Name:pol
OrganismiFeline immunodeficiency virus (isolate TM2) (FIV)
Taxonomic identifieri31676 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusFeline lentivirus group
Virus hostiFelidae (cat family) [TaxID: 9681]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 153153ProteaseBy similarityPRO_0000038849Add
BLAST
Chaini154 – 709556Reverse transcriptase/ribonuclease HBy similarityPRO_0000038850Add
BLAST
Chaini710 – 843134Deoxyuridine 5'-triphosphate nucleotidohydrolaseBy similarityPRO_0000038851Add
BLAST
Chaini844 – 1124281IntegraseBy similarityPRO_0000038852Add
BLAST

Post-translational modificationi

Cleavage sites that yield the mature proteins remain to be determined.

Structurei

3D structure databases

ProteinModelPortaliP31822.
SMRiP31822. Positions 41-153, 710-843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 14382Peptidase A2PROSITE-ProRule annotationAdd
BLAST
Domaini199 – 388190Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST
Domaini591 – 711121RNase HPROSITE-ProRule annotationAdd
BLAST
Domaini899 – 1049151Integrase catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated
Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
Contains 1 integrase-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 integrase-type zinc finger.PROSITE-ProRule annotation
Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
Contains 1 RNase H domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri848 – 88942Integrase-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
2.70.40.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF51283. SSF51283. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31822-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
ENLGKREGGA SCSPSKPSAA NSTICTSNGG ETIRFINYNT IGTTTTLERR
60 70 80 90 100
PEIQIFVNGH PIKFLLDTGA DITILNRKDF QIGNSIENGK QNMIGVGGGK
110 120 130 140 150
RGTNYINVHL EIRDENYRMQ CIFGNVCVLE DNSLIQPLLG RDNMIKFNIR
160 170 180 190 200
LVMAQISEKI PIVKVRMKDP TQGPQVKQWP LSNEKIEALT DIVERLESEG
210 220 230 240 250
KVKRADPNNP WNTPVFAIKK KSGKWRMLID FRVLNKLTDK GAEVQLGLPH
260 270 280 290 300
PAGLQMKKQV TVLDIGDAYF TIPLDPDYAP YTAFTLPRKN NAGPGRRYVW
310 320 330 340 350
CSLPQGWVLS PLIYQSTLNN ILQPFIKQNS ELDIYQYMDD IYIGSNLNKK
360 370 380 390 400
EHKQKVEELR KLLLWWGFET PEDKLQEEPP YKWMGYELHP LTWSIQQKQL
410 420 430 440 450
EIPERPTLNE LQKLAGKINW ASQTIPDLSI KELTNMMRGD QKLDSIREWT
460 470 480 490 500
VEAKREVQKA KEAIETQAQL NYYDPNRGLY AKLSLVGPHQ ICYQVYQKNP
510 520 530 540 550
EHILWYGKIN RQKKKAENTC DIALRACYKI REESIIRIGK EPVYEIPASR
560 570 580 590 600
EAWESNLIRS PYLKAPPPEV EFIHAALSIK RALSMIQDAP IIGAETWYID
610 620 630 640 650
GSRKQGKAAR AAYWTNTGKW QIMEIEGSNQ KAEVQALLLA LKAGSEEMNI
660 670 680 690 700
ITDSQYILNI LNQQPDLMEG LWQEVLEQME KKIAIFIDWV PGHKGIPGNE
710 720 730 740 750
EVDKLCQTMM IIEGEGILEK RSEDAGYDLL AAAQETHFLP GEVRIVPTKT
760 770 780 790 800
RIMLPKGHWG LIMGKSSIGS KGVDVLGGVI DEGYRGELGV IMINLTKKSI
810 820 830 840 850
TILEKQKIAQ LIILPCRHEG LQQGEIQMNS ERGEKGFGSA GVFSSWVDRI
860 870 880 890 900
EEAELNHEKF HSDPQYLRTE FNLPRIVAEE IKRKCPLCRI RGEQVGGQLK
910 920 930 940 950
IRPGIWQMDC THFNGKIIIV AVHVESGFLW AQIIPQETAD CTVKALLQLI
960 970 980 990 1000
CAHNVTELQT DNGPNFKNQK MEGLLNYMGI KHKLGIPGNP QSQALVENAN
1010 1020 1030 1040 1050
NTLKVWIQKF LPETTSLDNA LALALHCLNF KQRGRLGRMA PYELYIQQES
1060 1070 1080 1090 1100
LRIQDYFSAI PQKLMMQWLY YKDQKDKKWK GPMRVEYWGQ GSVLLKDEEK
1110 1120
GIFLVPRRHI RRVPEPCTLP EGDE
Length:1,124
Mass (Da):128,101
Last modified:July 1, 1993 - v1
Checksum:iF287FE90FC978296
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59418 Genomic RNA. Translation: AAA43071.1.
PIRiB45557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59418 Genomic RNA. Translation: AAA43071.1 .
PIRi B45557.

3D structure databases

ProteinModelPortali P31822.
SMRi P31822. Positions 41-153, 710-843.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi A02.007.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
2.70.40.10. 1 hit.
3.30.420.10. 2 hits.
InterProi IPR001969. Aspartic_peptidase_AS.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view ]
SUPFAMi SSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF51283. SSF51283. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of feline immunodeficiency virus rev gene activity."
    Kiyomasu T., Miyazawa T., Furuya T., Shibata R., Sakai H., Sakuragi J.I., Fukasawa M., Maki N., Hasegawa A., Mikami T., Adachi A.
    J. Virol. 65:4539-4542(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Molecular characterization and heterogeneity of feline immunodeficiency virus isolates."
    Maki N., Miyazawa T., Fukasawa M., Hasegawa A., Hayami M., Miki K., Mikami T.
    Arch. Virol. 123:29-45(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOL_FIVT2
AccessioniPrimary (citable) accession number: P31822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3