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P31822 (POL_FIVT2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pol polyprotein

Cleaved into the following 4 chains:

  1. Protease
    EC=3.4.23.-
    Alternative name(s):
    Retropepsin
  2. Reverse transcriptase/ribonuclease H
    Short name=RT
    EC=2.7.7.49
    EC=3.1.26.13
    Alternative name(s):
    Exoribonuclease H
    EC=3.1.13.2
  3. Deoxyuridine 5'-triphosphate nucleotidohydrolase
    Short name=dUTPase
    EC=3.6.1.23
  4. Integrase
    Short name=IN
Gene names
Name:pol
OrganismFeline immunodeficiency virus (isolate TM2) (FIV)
Taxonomic identifier31676 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusFeline lentivirus group
Virus hostFelidae (cat family) [TaxID: 9681]

Protein attributes

Sequence length1124 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activity

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.

dUTP + H2O = dUMP + diphosphate.

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

Cleavage sites that yield the mature proteins remain to be determined.

Sequence similarities

Belongs to the retroviral Pol polyprotein family.

Contains 1 integrase catalytic domain.

Contains 1 integrase-type DNA-binding domain.

Contains 1 integrase-type zinc finger.

Contains 1 peptidase A2 domain.

Contains 1 reverse transcriptase domain.

Contains 1 RNase H domain.

Ontologies

Keywords
   Biological processDNA integration
DNA recombination
Nucleotide metabolism
Viral genome integration
Virus entry into host cell
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionAspartyl protease
Endonuclease
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed DNA polymerase
Transferase
   Technical termMultifunctional enzyme
Gene Ontology (GO)
   Biological_processDNA integration

Inferred from electronic annotation. Source: UniProtKB-KW

DNA recombination

Inferred from electronic annotation. Source: UniProtKB-KW

dUTP metabolic process

Inferred from electronic annotation. Source: InterPro

establishment of integrated proviral latency

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

viral entry into host cell

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

RNA-DNA hybrid ribonuclease activity

Inferred from electronic annotation. Source: InterPro

RNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

aspartic-type endopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

dUTP diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

exoribonuclease H activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 153153Protease By similarity
PRO_0000038849
Chain154 – 709556Reverse transcriptase/ribonuclease H By similarity
PRO_0000038850
Chain710 – 843134Deoxyuridine 5'-triphosphate nucleotidohydrolase By similarity
PRO_0000038851
Chain844 – 1124281Integrase By similarity
PRO_0000038852

Regions

Domain62 – 14382Peptidase A2
Domain199 – 388190Reverse transcriptase
Domain591 – 711121RNase H
Domain899 – 1049151Integrase catalytic
Zinc finger848 – 88942Integrase-type
DNA binding1067 – 111549Integrase-type

Sites

Active site671For protease activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P31822 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: F287FE90FC978296

FASTA1,124128,101
        10         20         30         40         50         60 
ENLGKREGGA SCSPSKPSAA NSTICTSNGG ETIRFINYNT IGTTTTLERR PEIQIFVNGH 

        70         80         90        100        110        120 
PIKFLLDTGA DITILNRKDF QIGNSIENGK QNMIGVGGGK RGTNYINVHL EIRDENYRMQ 

       130        140        150        160        170        180 
CIFGNVCVLE DNSLIQPLLG RDNMIKFNIR LVMAQISEKI PIVKVRMKDP TQGPQVKQWP 

       190        200        210        220        230        240 
LSNEKIEALT DIVERLESEG KVKRADPNNP WNTPVFAIKK KSGKWRMLID FRVLNKLTDK 

       250        260        270        280        290        300 
GAEVQLGLPH PAGLQMKKQV TVLDIGDAYF TIPLDPDYAP YTAFTLPRKN NAGPGRRYVW 

       310        320        330        340        350        360 
CSLPQGWVLS PLIYQSTLNN ILQPFIKQNS ELDIYQYMDD IYIGSNLNKK EHKQKVEELR 

       370        380        390        400        410        420 
KLLLWWGFET PEDKLQEEPP YKWMGYELHP LTWSIQQKQL EIPERPTLNE LQKLAGKINW 

       430        440        450        460        470        480 
ASQTIPDLSI KELTNMMRGD QKLDSIREWT VEAKREVQKA KEAIETQAQL NYYDPNRGLY 

       490        500        510        520        530        540 
AKLSLVGPHQ ICYQVYQKNP EHILWYGKIN RQKKKAENTC DIALRACYKI REESIIRIGK 

       550        560        570        580        590        600 
EPVYEIPASR EAWESNLIRS PYLKAPPPEV EFIHAALSIK RALSMIQDAP IIGAETWYID 

       610        620        630        640        650        660 
GSRKQGKAAR AAYWTNTGKW QIMEIEGSNQ KAEVQALLLA LKAGSEEMNI ITDSQYILNI 

       670        680        690        700        710        720 
LNQQPDLMEG LWQEVLEQME KKIAIFIDWV PGHKGIPGNE EVDKLCQTMM IIEGEGILEK 

       730        740        750        760        770        780 
RSEDAGYDLL AAAQETHFLP GEVRIVPTKT RIMLPKGHWG LIMGKSSIGS KGVDVLGGVI 

       790        800        810        820        830        840 
DEGYRGELGV IMINLTKKSI TILEKQKIAQ LIILPCRHEG LQQGEIQMNS ERGEKGFGSA 

       850        860        870        880        890        900 
GVFSSWVDRI EEAELNHEKF HSDPQYLRTE FNLPRIVAEE IKRKCPLCRI RGEQVGGQLK 

       910        920        930        940        950        960 
IRPGIWQMDC THFNGKIIIV AVHVESGFLW AQIIPQETAD CTVKALLQLI CAHNVTELQT 

       970        980        990       1000       1010       1020 
DNGPNFKNQK MEGLLNYMGI KHKLGIPGNP QSQALVENAN NTLKVWIQKF LPETTSLDNA 

      1030       1040       1050       1060       1070       1080 
LALALHCLNF KQRGRLGRMA PYELYIQQES LRIQDYFSAI PQKLMMQWLY YKDQKDKKWK 

      1090       1100       1110       1120 
GPMRVEYWGQ GSVLLKDEEK GIFLVPRRHI RRVPEPCTLP EGDE 

« Hide

References

[1]"Identification of feline immunodeficiency virus rev gene activity."
Kiyomasu T., Miyazawa T., Furuya T., Shibata R., Sakai H., Sakuragi J.I., Fukasawa M., Maki N., Hasegawa A., Mikami T., Adachi A.
J. Virol. 65:4539-4542(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Molecular characterization and heterogeneity of feline immunodeficiency virus isolates."
Maki N., Miyazawa T., Fukasawa M., Hasegawa A., Hayami M., Miki K., Mikami T.
Arch. Virol. 123:29-45(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59418 Genomic RNA. Translation: AAA43071.1.
PIRB45557.

3D structure databases

ProteinModelPortalP31822.
SMRP31822. Positions 41-153, 710-843.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSA02.007.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProIPR001969. Aspartic_peptidase_AS.
IPR008180. dUTP_pyroPase.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view]
SUPFAMSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOL_FIVT2
AccessionPrimary (citable) accession number: P31822
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries