SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P31822

- POL_FIVT2

UniProt

P31822 - POL_FIVT2

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Pol polyprotein

Gene
pol
Organism
Feline immunodeficiency virus (isolate TM2) (FIV)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
dUTP + H2O = dUMP + diphosphate.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671For protease activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri848 – 88942Integrase-typeAdd
BLAST
DNA bindingi1067 – 111549Integrase-typeAdd
BLAST

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. dUTP diphosphatase activity Source: UniProtKB-EC
  4. exoribonuclease H activity Source: UniProtKB-EC
  5. RNA binding Source: InterPro
  6. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  7. RNA-DNA hybrid ribonuclease activity Source: InterPro
  8. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. DNA recombination Source: UniProtKB-KW
  3. dUTP metabolic process Source: InterPro
  4. establishment of integrated proviral latency Source: UniProtKB-KW
  5. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Nucleotide metabolism, Viral genome integration, Virus entry into host cell

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Protein family/group databases

MEROPSiA02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 4 chains:
Alternative name(s):
Retropepsin
Alternative name(s):
Exoribonuclease H (EC:3.1.13.2)
Integrase
Short name:
IN
Gene namesi
Name:pol
OrganismiFeline immunodeficiency virus (isolate TM2) (FIV)
Taxonomic identifieri31676 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusFeline lentivirus group
Virus hostiFelidae (cat family) [TaxID: 9681]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 153153Protease By similarityPRO_0000038849Add
BLAST
Chaini154 – 709556Reverse transcriptase/ribonuclease H By similarityPRO_0000038850Add
BLAST
Chaini710 – 843134Deoxyuridine 5'-triphosphate nucleotidohydrolase By similarityPRO_0000038851Add
BLAST
Chaini844 – 1124281Integrase By similarityPRO_0000038852Add
BLAST

Post-translational modificationi

Cleavage sites that yield the mature proteins remain to be determined.

Structurei

3D structure databases

ProteinModelPortaliP31822.
SMRiP31822. Positions 41-153, 710-843.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 14382Peptidase A2Add
BLAST
Domaini199 – 388190Reverse transcriptaseAdd
BLAST
Domaini591 – 711121RNase HAdd
BLAST
Domaini899 – 1049151Integrase catalyticAdd
BLAST

Sequence similaritiesi

Contains 1 RNase H domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri848 – 88942Integrase-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
2.70.40.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF51283. SSF51283. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31822-1 [UniParc]FASTAAdd to Basket

« Hide

ENLGKREGGA SCSPSKPSAA NSTICTSNGG ETIRFINYNT IGTTTTLERR     50
PEIQIFVNGH PIKFLLDTGA DITILNRKDF QIGNSIENGK QNMIGVGGGK 100
RGTNYINVHL EIRDENYRMQ CIFGNVCVLE DNSLIQPLLG RDNMIKFNIR 150
LVMAQISEKI PIVKVRMKDP TQGPQVKQWP LSNEKIEALT DIVERLESEG 200
KVKRADPNNP WNTPVFAIKK KSGKWRMLID FRVLNKLTDK GAEVQLGLPH 250
PAGLQMKKQV TVLDIGDAYF TIPLDPDYAP YTAFTLPRKN NAGPGRRYVW 300
CSLPQGWVLS PLIYQSTLNN ILQPFIKQNS ELDIYQYMDD IYIGSNLNKK 350
EHKQKVEELR KLLLWWGFET PEDKLQEEPP YKWMGYELHP LTWSIQQKQL 400
EIPERPTLNE LQKLAGKINW ASQTIPDLSI KELTNMMRGD QKLDSIREWT 450
VEAKREVQKA KEAIETQAQL NYYDPNRGLY AKLSLVGPHQ ICYQVYQKNP 500
EHILWYGKIN RQKKKAENTC DIALRACYKI REESIIRIGK EPVYEIPASR 550
EAWESNLIRS PYLKAPPPEV EFIHAALSIK RALSMIQDAP IIGAETWYID 600
GSRKQGKAAR AAYWTNTGKW QIMEIEGSNQ KAEVQALLLA LKAGSEEMNI 650
ITDSQYILNI LNQQPDLMEG LWQEVLEQME KKIAIFIDWV PGHKGIPGNE 700
EVDKLCQTMM IIEGEGILEK RSEDAGYDLL AAAQETHFLP GEVRIVPTKT 750
RIMLPKGHWG LIMGKSSIGS KGVDVLGGVI DEGYRGELGV IMINLTKKSI 800
TILEKQKIAQ LIILPCRHEG LQQGEIQMNS ERGEKGFGSA GVFSSWVDRI 850
EEAELNHEKF HSDPQYLRTE FNLPRIVAEE IKRKCPLCRI RGEQVGGQLK 900
IRPGIWQMDC THFNGKIIIV AVHVESGFLW AQIIPQETAD CTVKALLQLI 950
CAHNVTELQT DNGPNFKNQK MEGLLNYMGI KHKLGIPGNP QSQALVENAN 1000
NTLKVWIQKF LPETTSLDNA LALALHCLNF KQRGRLGRMA PYELYIQQES 1050
LRIQDYFSAI PQKLMMQWLY YKDQKDKKWK GPMRVEYWGQ GSVLLKDEEK 1100
GIFLVPRRHI RRVPEPCTLP EGDE 1124
Length:1,124
Mass (Da):128,101
Last modified:July 1, 1993 - v1
Checksum:iF287FE90FC978296
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59418 Genomic RNA. Translation: AAA43071.1.
PIRiB45557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59418 Genomic RNA. Translation: AAA43071.1 .
PIRi B45557.

3D structure databases

ProteinModelPortali P31822.
SMRi P31822. Positions 41-153, 710-843.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi A02.007.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
2.70.40.10. 1 hit.
3.30.420.10. 2 hits.
InterProi IPR001969. Aspartic_peptidase_AS.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view ]
SUPFAMi SSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF51283. SSF51283. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of feline immunodeficiency virus rev gene activity."
    Kiyomasu T., Miyazawa T., Furuya T., Shibata R., Sakai H., Sakuragi J.I., Fukasawa M., Maki N., Hasegawa A., Mikami T., Adachi A.
    J. Virol. 65:4539-4542(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Molecular characterization and heterogeneity of feline immunodeficiency virus isolates."
    Maki N., Miyazawa T., Fukasawa M., Hasegawa A., Hayami M., Miki K., Mikami T.
    Arch. Virol. 123:29-45(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOL_FIVT2
AccessioniPrimary (citable) accession number: P31822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi