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Protein

Pol polyprotein

Gene

pol

Organism
Feline immunodeficiency virus (isolate TM2) (FIV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
dUTP + H2O = dUMP + diphosphate.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67For protease activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri848 – 889Integrase-typePROSITE-ProRule annotationAdd BLAST42
DNA bindingi1067 – 1115Integrase-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, DNA-binding, Endonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase
Biological processDNA integration, DNA recombination, Nucleotide metabolism, Viral genome integration, Virus entry into host cell
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiA02.007

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 4 chains:
Alternative name(s):
Retropepsin
Alternative name(s):
Exoribonuclease H (EC:3.1.13.2)
Integrase (EC:2.7.7.-By similarity, EC:3.1.-.-By similarity)
Short name:
IN
Gene namesi
Name:pol
OrganismiFeline immunodeficiency virus (isolate TM2) (FIV)
Taxonomic identifieri31676 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusFeline lentivirus group
Virus hostiFelidae (cat family) [TaxID: 9681]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000388491 – 153ProteaseBy similarityAdd BLAST153
ChainiPRO_0000038850154 – 709Reverse transcriptase/ribonuclease HBy similarityAdd BLAST556
ChainiPRO_0000038851710 – 843Deoxyuridine 5'-triphosphate nucleotidohydrolaseBy similarityAdd BLAST134
ChainiPRO_0000038852844 – 1124IntegraseBy similarityAdd BLAST281

Post-translational modificationi

Cleavage sites that yield the mature proteins remain to be determined.

Structurei

3D structure databases

ProteinModelPortaliP31822
SMRiP31822
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 143Peptidase A2PROSITE-ProRule annotationAdd BLAST82
Domaini199 – 388Reverse transcriptasePROSITE-ProRule annotationAdd BLAST190
Domaini591 – 711RNase HPROSITE-ProRule annotationAdd BLAST121
Domaini899 – 1049Integrase catalyticPROSITE-ProRule annotationAdd BLAST151

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri848 – 889Integrase-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Family and domain databases

CDDicd05482 HIV_retropepsin_like, 1 hit
cd07557 trimeric_dUTPase, 1 hit
Gene3Di1.10.10.200, 1 hit
2.30.30.10, 1 hit
2.40.70.10, 1 hit
2.70.40.10, 1 hit
3.30.420.10, 2 hits
InterProiView protein in InterPro
IPR001969 Aspartic_peptidase_AS
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR033704 dUTPase_trimeric
IPR017856 Integrase-like_N
IPR036862 Integrase_C_dom_sf_retrovir
IPR001037 Integrase_C_retrovir
IPR001584 Integrase_cat-core
IPR003308 Integrase_Zn-bd_dom_N
IPR001995 Peptidase_A2_cat
IPR021109 Peptidase_aspartic_dom_sf
IPR034170 Retropepsin-like_cat_dom
IPR018061 Retropepsins
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
IPR000477 RT_dom
IPR010659 RVT_connect
IPR010661 RVT_thumb
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
PF00552 IN_DBD_C, 1 hit
PF02022 Integrase_Zn, 1 hit
PF00075 RNase_H, 1 hit
PF00665 rve, 1 hit
PF00077 RVP, 1 hit
PF00078 RVT_1, 1 hit
PF06815 RVT_connect, 1 hit
PF06817 RVT_thumb, 1 hit
SUPFAMiSSF46919 SSF46919, 1 hit
SSF50122 SSF50122, 1 hit
SSF50630 SSF50630, 1 hit
SSF51283 SSF51283, 1 hit
SSF53098 SSF53098, 2 hits
PROSITEiView protein in PROSITE
PS50175 ASP_PROT_RETROV, 1 hit
PS00141 ASP_PROTEASE, 1 hit
PS50994 INTEGRASE, 1 hit
PS51027 INTEGRASE_DBD, 1 hit
PS50879 RNASE_H, 1 hit
PS50878 RT_POL, 1 hit
PS50876 ZF_INTEGRASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ENLGKREGGA SCSPSKPSAA NSTICTSNGG ETIRFINYNT IGTTTTLERR
60 70 80 90 100
PEIQIFVNGH PIKFLLDTGA DITILNRKDF QIGNSIENGK QNMIGVGGGK
110 120 130 140 150
RGTNYINVHL EIRDENYRMQ CIFGNVCVLE DNSLIQPLLG RDNMIKFNIR
160 170 180 190 200
LVMAQISEKI PIVKVRMKDP TQGPQVKQWP LSNEKIEALT DIVERLESEG
210 220 230 240 250
KVKRADPNNP WNTPVFAIKK KSGKWRMLID FRVLNKLTDK GAEVQLGLPH
260 270 280 290 300
PAGLQMKKQV TVLDIGDAYF TIPLDPDYAP YTAFTLPRKN NAGPGRRYVW
310 320 330 340 350
CSLPQGWVLS PLIYQSTLNN ILQPFIKQNS ELDIYQYMDD IYIGSNLNKK
360 370 380 390 400
EHKQKVEELR KLLLWWGFET PEDKLQEEPP YKWMGYELHP LTWSIQQKQL
410 420 430 440 450
EIPERPTLNE LQKLAGKINW ASQTIPDLSI KELTNMMRGD QKLDSIREWT
460 470 480 490 500
VEAKREVQKA KEAIETQAQL NYYDPNRGLY AKLSLVGPHQ ICYQVYQKNP
510 520 530 540 550
EHILWYGKIN RQKKKAENTC DIALRACYKI REESIIRIGK EPVYEIPASR
560 570 580 590 600
EAWESNLIRS PYLKAPPPEV EFIHAALSIK RALSMIQDAP IIGAETWYID
610 620 630 640 650
GSRKQGKAAR AAYWTNTGKW QIMEIEGSNQ KAEVQALLLA LKAGSEEMNI
660 670 680 690 700
ITDSQYILNI LNQQPDLMEG LWQEVLEQME KKIAIFIDWV PGHKGIPGNE
710 720 730 740 750
EVDKLCQTMM IIEGEGILEK RSEDAGYDLL AAAQETHFLP GEVRIVPTKT
760 770 780 790 800
RIMLPKGHWG LIMGKSSIGS KGVDVLGGVI DEGYRGELGV IMINLTKKSI
810 820 830 840 850
TILEKQKIAQ LIILPCRHEG LQQGEIQMNS ERGEKGFGSA GVFSSWVDRI
860 870 880 890 900
EEAELNHEKF HSDPQYLRTE FNLPRIVAEE IKRKCPLCRI RGEQVGGQLK
910 920 930 940 950
IRPGIWQMDC THFNGKIIIV AVHVESGFLW AQIIPQETAD CTVKALLQLI
960 970 980 990 1000
CAHNVTELQT DNGPNFKNQK MEGLLNYMGI KHKLGIPGNP QSQALVENAN
1010 1020 1030 1040 1050
NTLKVWIQKF LPETTSLDNA LALALHCLNF KQRGRLGRMA PYELYIQQES
1060 1070 1080 1090 1100
LRIQDYFSAI PQKLMMQWLY YKDQKDKKWK GPMRVEYWGQ GSVLLKDEEK
1110 1120
GIFLVPRRHI RRVPEPCTLP EGDE
Length:1,124
Mass (Da):128,101
Last modified:July 1, 1993 - v1
Checksum:iF287FE90FC978296
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59418 Genomic RNA Translation: AAA43071.1
PIRiB45557

Similar proteinsi

Entry informationi

Entry nameiPOL_FIVT2
AccessioniPrimary (citable) accession number: P31822
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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