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Protein

Nitrate/nitrite response regulator protein NarP

Gene

narP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein activates the expression of the nitrate reductase (narGHJI) and formate dehydrogenase-N (fdnGHI) operons and represses the transcription of the fumarate reductase (frdABCD) operon in response to a nitrate/nitrite induction signal transmitted by either the NarX or NarQ proteins.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi171 – 19020H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Nitrate assimilation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NARP-MONOMER.
ECOL316407:JW2181-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate/nitrite response regulator protein NarP
Gene namesi
Name:narP
Ordered Locus Names:b2193, JW2181
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11527. narP.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Nitrate/nitrite response regulator protein NarPPRO_0000081149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei59 – 5914-aspartylphosphatePROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP31802.
PRIDEiP31802.

Interactioni

Protein-protein interaction databases

BioGridi4263485. 7 interactions.
DIPiDIP-10317N.
IntActiP31802. 5 interactions.
STRINGi511145.b2193.

Structurei

3D structure databases

ProteinModelPortaliP31802.
SMRiP31802. Positions 6-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 124117Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini147 – 21266HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105WH1. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000034813.
InParanoidiP31802.
KOiK07685.
OMAiYRNKQIA.
OrthoDBiEOG69GZGV.
PhylomeDBiP31802.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEATPFQVM IVDDHPLMRR GVRQLLELDP GSEVVAEAGD GASAIDLANR
60 70 80 90 100
LDIDVILLDL NMKGMSGLDT LNALRRDGVT AQIIILTVSD ASSDVFALID
110 120 130 140 150
AGADGYLLKD SDPEVLLEAI RAGAKGSKVF SERVNQYLRE REMFGAEEDP
160 170 180 190 200
FSVLTERELD VLHELAQGLS NKQIASVLNI SEQTVKVHIR NLLRKLNVRS
210
RVAATILFLQ QRGAQ
Length:215
Mass (Da):23,575
Last modified:July 1, 1993 - v1
Checksum:iC7121668E3130956
GO

Sequence cautioni

The sequence AAA16411.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11273 Genomic DNA. Translation: AAA24200.1.
U00008 Genomic DNA. Translation: AAA16411.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75253.1.
AP009048 Genomic DNA. Translation: BAE76656.1.
PIRiA40584.
RefSeqiNP_416697.1. NC_000913.3.
WP_001113639.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75253; AAC75253; b2193.
BAE76656; BAE76656; BAE76656.
GeneIDi949081.
KEGGiecj:JW2181.
eco:b2193.
PATRICi32119745. VBIEscCol129921_2282.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11273 Genomic DNA. Translation: AAA24200.1.
U00008 Genomic DNA. Translation: AAA16411.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75253.1.
AP009048 Genomic DNA. Translation: BAE76656.1.
PIRiA40584.
RefSeqiNP_416697.1. NC_000913.3.
WP_001113639.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31802.
SMRiP31802. Positions 6-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263485. 7 interactions.
DIPiDIP-10317N.
IntActiP31802. 5 interactions.
STRINGi511145.b2193.

Proteomic databases

PaxDbiP31802.
PRIDEiP31802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75253; AAC75253; b2193.
BAE76656; BAE76656; BAE76656.
GeneIDi949081.
KEGGiecj:JW2181.
eco:b2193.
PATRICi32119745. VBIEscCol129921_2282.

Organism-specific databases

EchoBASEiEB1489.
EcoGeneiEG11527. narP.

Phylogenomic databases

eggNOGiENOG4105WH1. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000034813.
InParanoidiP31802.
KOiK07685.
OMAiYRNKQIA.
OrthoDBiEOG69GZGV.
PhylomeDBiP31802.

Enzyme and pathway databases

BioCyciEcoCyc:NARP-MONOMER.
ECOL316407:JW2181-MONOMER.

Miscellaneous databases

PROiP31802.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12."
    Rabin R.S., Stewart V.
    J. Bacteriol. 175:3259-3268(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiNARP_ECOLI
AccessioniPrimary (citable) accession number: P31802
Secondary accession number(s): Q2MAQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: January 20, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.