Calcium/proton antiporter - Escherichia coli (strain K12)

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P31801 (CHAA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Calcium/proton antiporter

Gene names

Name:	chaA
Ordered Locus Names:	b1216, JW1207

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	According to Ref.1 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/proton antiporter activity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Miscellaneous	Pronounced pH dependence. Inhibition by magnesium.
Sequence similarities	Belongs to the sodium/potassium/calcium exchanger family.

Ontologies

Keywords
Biological process	Antiport Ion transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Calcium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular potassium ion homeostasis Inferred from genetic interaction PubMed 16687400. Source: EcoCyc cellular sodium ion homeostasis Inferred from genetic interaction PubMed 10398838. Source: EcoCyc
Cellular_component	integral to plasma membrane Inferred from direct assay PubMed 20196076. Source: EcoliWiki
Molecular_function	potassium:hydrogen antiporter activity Inferred from direct assay PubMed 12460671. Source: EcoCyc sodium:hydrogen antiporter activity Inferred from direct assay Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 366

366

Calcium/proton antiporter

PRO_0000209506

Regions

Topological domain

1 – 16

Cytoplasmic Potential

Transmembrane

17 – 37

Helical; Potential

Transmembrane

38 – 58

Helical; Potential

Topological domain

59 – 74

Cytoplasmic Potential

Transmembrane

75 – 95

Helical; Potential

Topological domain

96 – 106

Periplasmic Potential

Transmembrane

107 – 127

Helical; Potential

Topological domain

128 – 143

Cytoplasmic Potential

Transmembrane

144 – 164

Helical; Potential

Topological domain

165 – 167

Periplasmic Potential

Transmembrane

168 – 188

Helical; Potential

Topological domain

189 – 216

Cytoplasmic Potential

Transmembrane

217 – 237

Helical; Potential

Topological domain

238 – 255

Periplasmic Potential

Transmembrane

256 – 276

Helical; Potential

Topological domain

277 – 290

Cytoplasmic Potential

Transmembrane

291 – 311

Helical; Potential

Topological domain

312 – 318

Periplasmic Potential

Transmembrane

319 – 339

Helical; Potential

Topological domain

340 – 345

Cytoplasmic Potential

Transmembrane

346 – 366

Helical; Potential

Compositional bias

200 – 209

Asp/Glu-rich (acidic)

Sequences

Sequence

Length

Mass (Da)

Tools

P31801 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: C16935AC6A6D811E
Show »

FASTA

366

39,168

        10         20         30         40         50         60 
MSNAQEAVKT RHKETSLIFP VLALVVLFLW GSSQTLPVVI AINLLALIGI LSSAFSVVRH 

        70         80         90        100        110        120 
ADVLAHRLGE PYGSLILSLS VVILEVSLIS ALMATGDAAP TLMRDTLYSI IMIVTGGLVG 

       130        140        150        160        170        180 
FSLLLGGRKF ATQYMNLFGI KQYLIALFPL AIIVLVFPMA LPAANFSTGQ ALLVALISAA 

       190        200        210        220        230        240 
MYGVFLLIQT KTHQSLFVYE HEDDSDDDDP HHGKPSAHSS LWHAIWLIIH LIAVIAVTKM 

       250        260        270        280        290        300 
NASSLETLLD SMNAPVAFTG FLVALLILSP EGLGALKAVL NNQVQRAMNL FFGSVLATIS 

       310        320        330        340        350        360 
LTVPVVTLIA FMTGNELQFA LGAPEMVVMV ASLVLCHISF STGRTNVLNG AAHLALFAAY 


LMTIFA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene." Ivey D.M., Guffanti A.A., Zemsky J., Pinner E., Karpel R., Padan E., Schuldiner S., Krulwich T.A. J. Biol. Chem. 268:11296-11303(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NM8191.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L28709 Genomic DNA. Translation: AAA20200.1. U00096 Genomic DNA. Translation: AAC74300.1. AP009048 Genomic DNA. Translation: BAA36074.1.
PIR	A46716.
RefSeq	NP_415734.1. NC_000913.2. YP_489486.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P31801.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1216.
Protein family/group databases
TCDB	2.A.19.1.1. Ca2+:cation antiporter (CaCA) family.
Proteomic databases
PaxDb	P31801.
PRIDE	P31801.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74300; AAC74300; b1216. BAA36074; BAA36074; BAA36074.
GeneID	12931126. 945790.
KEGG	ecj:Y75_p1191. eco:b1216.
PATRIC	32117690. VBIEscCol129921_1267.
Organism-specific databases
EchoBASE	EB1703.
EcoGene	EG11753. chaA.
Phylogenomic databases
eggNOG	COG0387.
HOGENOM	HOG000197726.
KO	K07300.
OMA	VWLIVHL.
ProtClustDB	PRK10599.
Enzyme and pathway databases
BioCyc	EcoCyc:CHAA-MONOMER. ECOL316407:JW1207-MONOMER.
Gene expression databases
Genevestigator	P31801.
Family and domain databases
InterPro	IPR004713. CaH_exchang. IPR004837. NaCa_Exmemb. [Graphical view]
Pfam	PF01699. Na_Ca_ex. 1 hit. [Graphical view]
TIGRFAMs	TIGR00846. caca2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CHAA_ECOLI

Accession

Primary (citable) accession number: P31801

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents