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Protein

Sodium-potassium/proton antiporter ChaA

Gene

chaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sodium exporter that functions mainly at alkaline pH. Can also function as a potassium/proton and calcium/proton antiporter at alkaline pH. Does not play a major role in calcium export.5 Publications

Enzyme regulationi

Pronounced pH dependence. Inhibited by magnesium.1 Publication

GO - Molecular functioni

  • potassium:proton antiporter activity Source: EcoCyc
  • sodium:proton antiporter activity Source: EcoCyc

GO - Biological processi

  • sodium ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Keywords - Ligandi

Calcium, Potassium, Sodium

Enzyme and pathway databases

BioCyciEcoCyc:CHAA-MONOMER.
ECOL316407:JW1207-MONOMER.
MetaCyc:CHAA-MONOMER.

Protein family/group databases

TCDBi2.A.19.1.1. the ca(2+):cation antiporter (caca) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-potassium/proton antiporter ChaACurated
Alternative name(s):
Na(+)/H(+) exchanger
Gene namesi
Name:chaA
Ordered Locus Names:b1216, JW1207
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11753. chaA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Topological domaini59 – 74CytoplasmicSequence analysisAdd BLAST16
Transmembranei75 – 95HelicalSequence analysisAdd BLAST21
Topological domaini96 – 106PeriplasmicSequence analysisAdd BLAST11
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 143CytoplasmicSequence analysisAdd BLAST16
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 167PeriplasmicSequence analysis3
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 216CytoplasmicSequence analysisAdd BLAST28
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Topological domaini238 – 255PeriplasmicSequence analysisAdd BLAST18
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Topological domaini277 – 290CytoplasmicSequence analysisAdd BLAST14
Transmembranei291 – 311HelicalSequence analysisAdd BLAST21
Topological domaini312 – 318PeriplasmicSequence analysis7
Transmembranei319 – 339HelicalSequence analysisAdd BLAST21
Topological domaini340 – 345CytoplasmicSequence analysis6
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002095061 – 366Sodium-potassium/proton antiporter ChaAAdd BLAST366

Proteomic databases

PaxDbiP31801.
PRIDEiP31801.

Interactioni

Protein-protein interaction databases

BioGridi4260120. 10 interactors.
STRINGi511145.b1216.

Structurei

3D structure databases

ProteinModelPortaliP31801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi200 – 209Asp/Glu-rich (acidic)10

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EWV. Bacteria.
COG0387. LUCA.
HOGENOMiHOG000197726.
InParanoidiP31801.
KOiK07300.
OMAiPEMIVMV.
PhylomeDBiP31801.

Family and domain databases

InterProiIPR004713. CaH_exchang.
IPR004837. NaCa_Exmemb.
[Graphical view]
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00846. caca2. 1 hit.

Sequencei

Sequence statusi: Complete.

P31801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNAQEAVKT RHKETSLIFP VLALVVLFLW GSSQTLPVVI AINLLALIGI
60 70 80 90 100
LSSAFSVVRH ADVLAHRLGE PYGSLILSLS VVILEVSLIS ALMATGDAAP
110 120 130 140 150
TLMRDTLYSI IMIVTGGLVG FSLLLGGRKF ATQYMNLFGI KQYLIALFPL
160 170 180 190 200
AIIVLVFPMA LPAANFSTGQ ALLVALISAA MYGVFLLIQT KTHQSLFVYE
210 220 230 240 250
HEDDSDDDDP HHGKPSAHSS LWHAIWLIIH LIAVIAVTKM NASSLETLLD
260 270 280 290 300
SMNAPVAFTG FLVALLILSP EGLGALKAVL NNQVQRAMNL FFGSVLATIS
310 320 330 340 350
LTVPVVTLIA FMTGNELQFA LGAPEMVVMV ASLVLCHISF STGRTNVLNG
360
AAHLALFAAY LMTIFA
Length:366
Mass (Da):39,168
Last modified:July 1, 1993 - v1
Checksum:iC16935AC6A6D811E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28709 Genomic DNA. Translation: AAA20200.1.
U00096 Genomic DNA. Translation: AAC74300.1.
AP009048 Genomic DNA. Translation: BAA36074.1.
PIRiA46716.
RefSeqiNP_415734.1. NC_000913.3.
WP_000063607.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74300; AAC74300; b1216.
BAA36074; BAA36074; BAA36074.
GeneIDi945790.
KEGGiecj:JW1207.
eco:b1216.
PATRICi32117690. VBIEscCol129921_1267.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28709 Genomic DNA. Translation: AAA20200.1.
U00096 Genomic DNA. Translation: AAC74300.1.
AP009048 Genomic DNA. Translation: BAA36074.1.
PIRiA46716.
RefSeqiNP_415734.1. NC_000913.3.
WP_000063607.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31801.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260120. 10 interactors.
STRINGi511145.b1216.

Protein family/group databases

TCDBi2.A.19.1.1. the ca(2+):cation antiporter (caca) family.

Proteomic databases

PaxDbiP31801.
PRIDEiP31801.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74300; AAC74300; b1216.
BAA36074; BAA36074; BAA36074.
GeneIDi945790.
KEGGiecj:JW1207.
eco:b1216.
PATRICi32117690. VBIEscCol129921_1267.

Organism-specific databases

EchoBASEiEB1703.
EcoGeneiEG11753. chaA.

Phylogenomic databases

eggNOGiENOG4105EWV. Bacteria.
COG0387. LUCA.
HOGENOMiHOG000197726.
InParanoidiP31801.
KOiK07300.
OMAiPEMIVMV.
PhylomeDBiP31801.

Enzyme and pathway databases

BioCyciEcoCyc:CHAA-MONOMER.
ECOL316407:JW1207-MONOMER.
MetaCyc:CHAA-MONOMER.

Miscellaneous databases

PROiP31801.

Family and domain databases

InterProiIPR004713. CaH_exchang.
IPR004837. NaCa_Exmemb.
[Graphical view]
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00846. caca2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCHAA_ECOLI
AccessioniPrimary (citable) accession number: P31801
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.