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P31796

- ENV_AVISN

UniProt

P31796 - ENV_AVISN

Protein

Envelope glycoprotein

Gene

env

Organism
Avian spleen necrosis virus
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    The surface protein (SU) attaches the virus to the host cell by binding to its receptor. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. Fusion occurs at the host cell plasma membrane By similarity.By similarity
    The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei398 – 3992Cleavage; by hostBy similarity

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envelope glycoprotein
    Alternative name(s):
    Env polyprotein
    Cleaved into the following 2 chains:
    Surface protein
    Short name:
    SU
    Alternative name(s):
    Glycoprotein 73
    Short name:
    gp73
    Transmembrane protein
    Short name:
    TM
    Alternative name(s):
    Glycoprotein 22
    Short name:
    gp22
    Gene namesi
    Name:env
    OrganismiAvian spleen necrosis virus
    Taxonomic identifieri11899 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
    Virus hostiGalliformes [TaxID: 8976]

    Subcellular locationi

    Chain Transmembrane protein : Virion membrane By similarity; Single-pass type I membrane protein By similarity. Host cell membrane By similarity; Single-pass type I membrane protein By similarity
    Note: It is probably concentrated at the site of budding and incorporated into the virions possibly by contacts between the cytoplasmic tail of Env and the N-terminus of Gag.By similarity
    Chain Surface protein : Virion membrane By similarity; Peripheral membrane protein By similarity. Host cell membrane By similarity; Peripheral membrane protein By similarity
    Note: The surface protein is not anchored to the viral envelope, but associates with the extravirion surface through its binding to TM. It is probably concentrated at the site of budding and incorporated into the virions possibly by contacts between the cytoplasmic tail of Env and the N-terminus of Gag By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3535Sequence AnalysisAdd
    BLAST
    Chaini36 – 567532Envelope glycoproteinPRO_0000239547Add
    BLAST
    Chaini36 – 398363Surface proteinBy similarityPRO_0000040685Add
    BLAST
    Chaini399 – 567169Transmembrane proteinBy similarityPRO_0000040686Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi245 – 2451N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi255 ↔ 487Interchain (between SU and TM chains, or C-258 with C-487); in linked formBy similarity
    Disulfide bondi255 ↔ 258By similarity
    Glycosylationi274 – 2741N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi280 – 2801N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi306 – 3061N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi319 – 3191N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi335 – 3351N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi479 ↔ 486By similarity
    Glycosylationi491 – 4911N-linked (GlcNAc...); by hostSequence Analysis
    Lipidationi549 – 5491S-palmitoyl cysteine; by hostBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins. Envelope glycoproteins are synthesized as a inactive precursor that is N-glycosylated and processed likely by host cell furin or by a furin-like protease in the Golgi to yield the mature SU and TM proteins. The cleavage site between SU and TM requires the minimal sequence [KR]-X-[KR]-R By similarity.By similarity
    The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion By similarity.By similarity
    The transmembrane protein is palmitoylated.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Interactioni

    Subunit structurei

    The mature envelope protein (Env) consists of a trimer of SU-TM heterodimers attached by a labile interchain disulfide bond.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP31796.
    SMRiP31796. Positions 439-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini36 – 525490ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini547 – 56721CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei526 – 54621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni402 – 42221Fusion peptideSequence AnalysisAdd
    BLAST
    Regioni462 – 47817ImmunosuppressionBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili423 – 47351Sequence AnalysisAdd
    BLAST
    Coiled coili483 – 51937Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi255 – 2584CXXC
    Motifi479 – 4879CX6CC

    Domaini

    The 17 amino acids long immunosuppressive region is present in many retroviral envelope proteins. Synthetic peptides derived from this relatively conserved sequence inhibit immune function in vitro and in vivo By similarity.By similarity

    Keywords - Domaini

    Coiled coil, Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR018154. TLV/ENV_coat_polyprotein.
    [Graphical view]
    PANTHERiPTHR10424. PTHR10424. 1 hit.
    PfamiPF00429. TLV_coat. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31796-1 [UniParc]FASTAAdd to Basket

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    MDCLTNLRSA EGKVDQASKI LILLVAWWGF GTTAEGYPLQ QLWELPCDCS    50
    GGYVSSIPTY YTYSLDCGGS TAYLTYGSGT GSWSWGGGFK QQWECVFKPK 100
    IIPSVQGQPG PCPSECLQIA TQMHSTCYEK TQECTLLGKT YFTAILQKTK 150
    LGSYEDGPNK LIQASCTGTV GKPVCWDPVA PVYVSDGGGP TDMIREESVR 200
    ERLEEIIRHS YPSVQYHPLA LPRSRGVDLD PQTSDILEAT HQVLNATNPK 250
    LAENCWLCMT LGTPIPAAIP TNGNVTLDGN CSLSLPFGCN PPGSIDVSCY 300
    AGEADNRTGI PVGYVHFTNC TSIQEVTNET SQMGNLTRLC PPPGHVFVCG 350
    NNMAYTALPN KWIGLCILAS IVPDISIISG EEPIPLPSIE YTARRHKRAV 400
    QFIPLLVGLG ISGATLAGGT GLGVSVHTYH KLSNQLIEDV QALSGTINDL 450
    QDQIDSLAEV VLQNRRGLDL LTAEQGGICL ALQEKCCFYA NKSGIVRDKI 500
    RKLQEDLIER KRALYDNPLW SGLNGFLPYL LPLLGPLFGL ILFLTLGPCI 550
    MKTLTRIIHD KIQAVKS 567
    Length:567
    Mass (Da):61,597
    Last modified:July 1, 1993 - v1
    Checksum:i204EA57C32159175
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87666 Genomic DNA. No translation available.
    PIRiA38212. VCFVAS.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M87666 Genomic DNA. No translation available.
    PIRi A38212. VCFVAS.

    3D structure databases

    ProteinModelPortali P31796.
    SMRi P31796. Positions 439-491.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR018154. TLV/ENV_coat_polyprotein.
    [Graphical view ]
    PANTHERi PTHR10424. PTHR10424. 1 hit.
    Pfami PF00429. TLV_coat. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Spleen necrosis virus, an avian immunosuppressive retrovirus, shares a receptor with the type D simian retroviruses."
      Kewalramani V.N., Panganiban A.T., Emerman M.
      J. Virol. 66:3026-3031(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Clone PPB101.

    Entry informationi

    Entry nameiENV_AVISN
    AccessioniPrimary (citable) accession number: P31796
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program