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P31793 (ENV_FRSFB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycoprotein 42

Short name=gp42
Gene names
Name:env
OrganismFriend spleen focus-forming virus (strain BB6) (FSFFV)
Taxonomic identifier31692 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

This envelope-like membrane glycoprotein is responsible for ligand-independent activation of the erythropoietin receptor EPOR leading to the abnormally rapid proliferation of erythroid precursor cells. In the first stage of Friend disease, constitutive activation of the EPOR by gp42 causes uncontrolled, polyclonal proliferation of infected erythroblasts, leading to polycythemia (massive increase in the number of mature red cells). Host susceptibility to SSFV-induced erythroblastosis usually depends on the expression of the truncated isoform ofMST1R receptor tyrosine kinase (MST1R isoform sf-Stk) but the deletion mutant BB6 apparently can overcome its absence.

Subunit structure

Homooligomer. Forms heterooligomers with mouse EPOR, probably via their respective transmembrane domains. BB6 deletion mutant does not interact with mouse MST1R isoform sf-Stk

Subcellular location

Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Virion membrane; Single-pass type I membrane protein. Note: The envelope-like membrane glycoprotein gp55 is defective in its transport to the cell surface and remains associated predominantly with the rough endoplasmic reticulum (RER) membrane. It is almost not incorporated into virions. Host cell surface expression appears to be a prerequisite for its leukemogenicity By similarity.

Miscellaneous

Compared to other gammaretroviruses which possess 2 envelope proteins (gp70 and p15E), FSFFV gp55 corresponds to a gp70-p15E fusion protein with a deletion of a portion of p15E. It is encoded by the defective env gene of the virus. Strain BB6 gp42 is a truncated form of gp55.

The Friend murine leukemia virus complex induces a rapid and fatal erythroleukemia in adult mice. It is the replication-defective spleen focus-forming virus (SFFV) contained in this complex that causes foci of proliferating erythroid cells in spleens of infected mice. The second component is a replication competent Friend murine leukemia virus (F-MuLV) that serves as a helper virus for SFFV.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 356324Glycoprotein 42
PRO_0000040722

Regions

Topological domain33 – 331299Virion surface Potential
Transmembrane332 – 35221Helical; Potential
Topological domain353 – 3564Intravirion Potential
Motif306 – 3094CXXC
Compositional bias229 – 27850Pro-rich

Amino acid modifications

Glycosylation431N-linked (GlcNAc...); by host Potential
Glycosylation581N-linked (GlcNAc...); by host Potential
Glycosylation2961N-linked (GlcNAc...); by host Potential
Glycosylation3281N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P31793 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 0C7893BE2794F980

FASTA35638,778
        10         20         30         40         50         60 
MEGPAFSKPL KDKINPWGPL IVLGILIRAG VSVQRDSPHQ VFNVTWRVTN LMTGQTANAT 

        70         80         90        100        110        120 
SLLGTMTDAF PKLYFDLCDL IGNDWDETRL GCRTPGEGKR ARTFDLYVCP GHTVPTGCGG 

       130        140        150        160        170        180 
PREGYCGKWG CETTGQAYWK PSSSWDLISL KRGNTPKDRG PCYDSSVSSG VQGATPGGRC 

       190        200        210        220        230        240 
NPLVLKFTDA GKKASWDAPK VWGLRLYRST GTDPVTRFSL TRQVLNIGPR VPIGPNPVIS 

       250        260        270        280        290        300 
DQLPPSRPAQ IMLPRPPQPP PPGTASIVPE TAPPSQQPGT RDRLLNLVNK AYQALNLTSP 

       310        320        330        340        350 
DKTQECWLCL VSRPPYYEGV AVLGTNSNHT TLISTIMGLL IILLLLLILL LWTLHS 

« Hide

References

[1]"Mutations in the env gene of friend spleen focus-forming virus overcome Fv-2r-mediated resistance to Friend virus-induced erythroleukemia."
Majumdar M.K., Cho C.L., Fox M.T., Eckner K.L., Kozak S., Kabat D., Geib R.W.
J. Virol. 66:3652-3660(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M90673 Genomic DNA. No translation available.
PIRVCVWB6. A41995.

3D structure databases

ProteinModelPortalP31793.
SMRP31793. Positions 38-229.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.90.310.10. 1 hit.
InterProIPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view]
PANTHERPTHR10424. PTHR10424. 1 hit.
PfamPF00429. TLV_coat. 2 hits.
[Graphical view]
SUPFAMSSF49830. SSF49830. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENV_FRSFB
AccessionPrimary (citable) accession number: P31793
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program