Reviewed, UniProtKB/Swiss-Prot P31792 (POL_FENV1)

Names and origin

Protein names

Recommended name:
    Pol polyprotein
Cleaved into the following 2 chains:
    1- Recommended name:
            Reverse transcriptase/ribonuclease H
                Short name=RT
              EC=2.7.7.49
              EC=2.7.7.7
              EC=3.1.26.4
    2- Recommended name:
            Integrase
                Short name=IN

Gene names

Name:

pol

Organism

Feline endogenous virus ECE1

Taxonomic identifier

11766 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › unclassified Gammaretrovirus

Virus host

Felidae (cat family) [TaxID: 9681]

Protein attributes

Sequence length	1046 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Endonucleolytic cleavage to 5'-phosphomonoester.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins.
Sequence similarities	Belongs to the retroviral Pol polyprotein family. Contains 1 integrase catalytic domain. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Molecular function	Endonuclease Hydrolase Nuclease Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: EC RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 647	›647	Reverse transcriptase/ribonuclease H		PRO_0000040980
	Chain	648 – 1046	399	Integrase		PRO_0000040981
Regions
	Domain	55 – 248	194	Reverse transcriptase
	Domain	491 – 637	147	RNase H
	Domain	757 – 915	159	Integrase catalytic
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P31792-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 84556FC1BF29E4A9	FASTA	1,046	117,101
	10 20 30 40 50 60 LQDFPQAWAE TGGLGRAKCQ VPIIIDLKPT AMPVSIRQYP MSKEAHMGIQ PHITRFLELG 70 80 90 100 110 120 VLRPCRSPWN TPLLPVKKPG TRDYRPVQDL REVNKRTMDI HPTVPNPYNL LSTLSPDRTW 130 140 150 160 170 180 YTVLDLKDAF FCLPLAPQSQ ELFAFEWRDP ERGISGQLTW TRLPQGFKNS PTLFDEALHR 190 200 210 220 230 240 DLTDFRTQHP EVTLLQYVDD LLLAAPTKEA CIRGTKHLLR ELGDKGYRAS AKKAQICQTK 250 260 270 280 290 300 VTYLGYILSE GKRWLTPGRI ETVAHIPPPQ NPREVREFLG TAGFCRLWIP GFAELAAPLY 310 320 330 340 350 360 ALTKESAPFT WQEKHQSAFE ALKEALLSAP ALGLPDTSKP FTLFIDEKQG IAKGVLTQKL 370 380 390 400 410 420 GPWKRPVAYL SKKLDPVAAG WPPCLRIMAA TAMLVKDSAK LTLGQPLTVI TPHALEAIVR 430 440 450 460 470 480 QTPDRWITNA RLTHYQALLL DTDRIQFGPP VTLNPATLLP APEDQQSAHD CRQVLAETHG 490 500 510 520 530 540 TREDLKDQEL PDADHSWYTD GSSYIDSGTR RAGAAVVDGH HIIWAQSLPP GTSAQKAELI 550 560 570 580 590 600 ALTKALELSE GKKANIYTDS RYAFATAHTH GSIYERRGLL TSEGKEIKNK AEIIALLKAL 610 620 630 640 650 660 FLPRKVAIIH CPGHQKGQDP IATGNRQADQ VARQVAVAET LTLTTKLEET NLTTNKYAYT 670 680 690 700 710 720 PEDQEEAKAI GAILNQDTKD WEKEGKIVLP RKEALAMIQQ MHAWTHLSNQ KLKLLIEKTD 730 740 750 760 770 780 FLIPKAGTLI EQVTSACKVC QQVNAGATRV PEGKRTRGNR PGVYWEIDFT EVKPHYAGYK 790 800 810 820 830 840 YLLVFVDTFS GWVEAYPTRQ ETAHMVAKKI LEEIFPRFGL PKVIGSDNGP AFVSQVSQGL 850 860 870 880 890 900 ARTLGINWKL HCAYRPQSSG QVERMNRTIK ETLTKLTLET GLKDWRRLLS LALLRARNTP 910 920 930 940 950 960 NRFGLTPYEI LYGGPPPLST LLNSFSPSDP KTDLQARLKG LQAVQAQIWT PLAELYRPGH 970 980 990 1000 1010 1020 PQTSYPFQVG DSVYVRWHRS QGLEPRWKGP YIVLLTTPTA IKVDGIAAWI HASHAKAAPK 1030 1040 TPGPETPKTW KLHRSENPLK IRLSRV

References

[1]	"The exogenous RD-114 and the related endogenous proviral element ECE1 of domestic cat differ in their env genes." Moehring R., Drescher B., Riedel H., Bauer D., Rohde K., Beyer W., Rosenthal S. Submitted (FEB-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X51929 Genomic DNA. Translation: CAB38566.1.
PIR	GNMVCE. S12813.
3D structure databases
SMR	P31792. Positions 1-449, 22-634, 479-640, 760-913.
ModBase	Search...
Family and domain databases
InterPro	IPR001584. Integrase_cat-core. IPR012337. PolynucTfrase_RNaseH_fold. IPR000477. Reverse_transcriptase. IPR002156. RNase_H. [Graphical view]
Pfam	PF00075. RnaseH. 1 hit. PF00665. rve. 1 hit. PF00078. RVT_1. 1 hit. [Graphical view]
PROSITE	PS50994. INTEGRASE. 1 hit. PS50879. RNASE_H. 1 hit. PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL_FENV1

Accession

Primary (citable) accession number: P31792
Secondary accession number(s): Q28415

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)