Reviewed, UniProtKB/Swiss-Prot P31787 (ACBP_YEAST)

Names and origin

Protein names

Recommended name:
    Acyl-CoA-binding protein
      Short name=ACBP

Gene names

Name:	ACB1
Synonyms:	ACB
Ordered Locus Names:	YGR037C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters.
Miscellaneous	Present with 8500 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the ACBP family. Contains 1 ACB (acyl-CoA-binding) domain.

Ontologies

Keywords
Biological process	Transport
Ligand	Lipid-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from direct assay. Source: SGD long-chain fatty acid transport Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	acyl-CoA binding Inferred from electronic annotation. Source: InterPro long-chain fatty acid transporter activity Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADE17	P38009	1	EBI-2060,EBI-14223
AGP1	P25376	1	EBI-2060,EBI-2357
ALO1	P54783	1	EBI-2060,EBI-2519
ARC40	P38328	1	EBI-2060,EBI-2777
ARO8	P53090	1	EBI-2060,EBI-2042933
ATG1	P53104	1	EBI-2060,EBI-2657
CYS4	P32582	1	EBI-2060,EBI-4167
GLN1	P32288	1	EBI-2060,EBI-7665
HEF3	P53978	1	EBI-2060,EBI-6345
HSP12	P22943	1	EBI-2060,EBI-8548
HUG1	Q6Q5K6	1	EBI-2060,EBI-392766
IPP1	P00817	1	EBI-2060,EBI-9338
MBF1	O14467	1	EBI-2060,EBI-2043025
OYE2	Q03558	1	EBI-2060,EBI-12729
PMP2	P40975	1	EBI-2060,EBI-2043041
PMP3	P87284	1	EBI-2060,EBI-13555
RNR1	P21524	1	EBI-2060,EBI-15234
RNR2	P09938	1	EBI-2060,EBI-15240
SEC26	P41810	1	EBI-2060,EBI-4869
SEC27	P41811	1	EBI-2060,EBI-4898
SWE1	P32944	1	EBI-2060,EBI-18607
UBA1	P22515	1	EBI-2060,EBI-19703
URN1	Q06525	1	EBI-2060,EBI-35138
VAS1	P07806-1	1	EBI-2060,EBI-1009942
VMA4	P22203	1	EBI-2060,EBI-20268
VMA8	P32610	1	EBI-2060,EBI-20264

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 87

86

Acyl-CoA-binding protein

PRO_0000214013

Regions

Domain

2 – 87

86

ACB

Secondary structure

1

.

.

.

.

.

.

.

.

.

87

Helix Strand Turn

Details...

Helix

3 – 13

11

Helix

22 – 37

16

Helix

50 – 60

11

Turn

61 – 65

5

Helix

66 – 85

20

Sequences

	Sequence		Length	Mass (Da)
	P31787-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 686B186195EA02FC	FASTA	87	10,061
	10 20 30 40 50 60 MVSQLFEEKA KAVNELPTKP STDELLELYA LYKQATVGDN DKEKPGIFNM KDRYKWEAWE 70 80 NLKGKSQEDA EKEYIALVDQ LIAKYSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the gene for the yeast homolog (ACB) of diazepam binding inhibitor/endozepine/acyl-CoA-binding protein." Rose T.M., Schultz E.R., Todaro G.J. Proc. Natl. Acad. Sci. U.S.A. 89:11287-11291(1992) [PubMed: 1454809] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Saccharomyces carlsbergensis contains two functional genes encoding the acyl-CoA binding protein, one similar to the ACB1 gene from S. cerevisiae and one identical to the ACB1 gene from S. monacensis." Borsting C., Hummel R., Schultz E.R., Rose T.M., Pedersen M.B., Knudsen J., Kristiansen K. Yeast 13:1409-1421(1997) [PubMed: 9434347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180.
[3]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed: 16273108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SK1.
[4]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Different secondary structure elements as scaffolds for protein folding transition states of two homologous four-helix bundles." Teilum K., Thormann T., Caterer N.R., Poulsen H.I., Jensen P.H., Knudsen J., Kragelund B.B., Poulsen F.M. Proteins 59:80-90(2005) [PubMed: 15690348] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M99489 Genomic DNA. Translation: AAA34384.1.
Y08687 Genomic DNA. Translation: CAA69944.1.
Y08689 Genomic DNA. Translation: CAA69947.1.
DQ115390 Genomic DNA. Translation: AAZ22453.1.
Z72822 Genomic DNA. Translation: CAA97025.1.

PIR

S31247.

RefSeq

NP_011551.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ST7	NMR	-	A	2-87	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P31787. 34 interactions.

STRING

P31787.

Proteomic databases

PeptideAtlas

P31787.

Genome annotation databases

Ensembl

YGR037C; YGR037C; YGR037C; Saccharomyces cerevisiae. [Genome view]

GeneID

852925.

GenomeReviews

Gene locus YGR037C in contig Y13135_GR.

KEGG

sce:YGR037C.

NMPDR

fig|4932.3.peg.2665.

Organism-specific databases

CYGD

YGR037c.

SGD

S000003269. ACB1.

Phylogenomic databases

HOGENOM

P31787.

OMA

LFKQASV.

Gene expression databases

ArrayExpress

P31787.

Genevestigator

P31787.

GermOnline

YGR037C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000582. Acyl-CoA-binding_protein.
IPR014352. FERM/acyl-CoA_bd_prot_3-hlx.
[Graphical view]

Gene3D

G3DSA:1.20.80.10. ACBP. 1 hit.

PANTHER

PTHR23310. ACBP. 1 hit.

Pfam

PF00887. ACBP. 1 hit.
[Graphical view]

PRINTS

PR00689. ACOABINDINGP.

ProDom

PD351532. Ac_coA_bind_prot. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

972643.

Entry information

Entry name

ACBP_YEAST

Accession

Primary (citable) accession number: P31787
Secondary accession number(s): Q45U46

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)