Reviewed, UniProtKB/Swiss-Prot P31754 (PYR5_BOVIN)

Names and origin

Protein names

Recommended name:
    Uridine 5'-monophosphate synthase
      Short name=UMP synthase
Including the following 2 domains:
    1- Recommended name:
            Orotate phosphoribosyltransferase
                Short name=OPRTase
              EC=2.4.2.10
    2- Recommended name:
            Orotidine 5'-phosphate decarboxylase
              EC=4.1.1.23
        Alternative name(s):
            OMPdecase

Gene names

Name:

UMPS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Orotidine 5'-phosphate = UMP + CO2.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. In the C-terminal section; belongs to the OMP decarboxylase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Glycosyltransferase Lyase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Uridine 5'-monophosphate synthase		PRO_0000139648
Regions
	Region	1 – 214	214	OPRTase
	Region	215 – 220	6	Domain linker
	Region	221 – 480	260	OMPdecase
Sites
	Active site	314	1	By similarity
	Active site	314	1	For OMPdecase activity By similarity
	Active site	317	1	For OMPdecase activity By similarity
Amino acid modifications
	Modified residue	37	1	Phosphotyrosine By similarity
	Modified residue	214	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	181	1	C → R in AAI12873. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P31754-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 7BDD5964C340940B	FASTA	480	52,229
	10 20 30 40 50 60 MAAADALLGS LVTGLYDVQA FKFGNFVLKS GLSSPVYIDL RGIISRPSIL NQVAEMLFQT 70 80 90 100 110 120 AENAEINFDT VCGVPYTALP LATIVCSTHE IPMLIRRKEK KDYGTKRLIE GAVNPGDTCL 130 140 150 160 170 180 IIEDVVSSGS SVWETAEVLQ KEGLKVTDAV VLVDREQGGR DNLQARGIRL HSVCTLSTVL 190 200 210 220 230 240 CILEQQKKIN AETVERVKRF IQENAFVAAN PNDSLPSVKK EPKELSFGAR AELPGTHPVA 250 260 270 280 290 300 AKLLRLMQKK ETNLCLSADV SESRELLQLA DALGSRICLL KIHVDILNDF TLDVMKELTT 310 320 330 340 350 360 LAKRHEFLIF EDRKFADIGN TVKKQYEGGV FKIASWADLV NAHAVPGSGV VKGLEEVGLP 370 380 390 400 410 420 LHRACLLVAE MSSAGTLATG SYTEAAVQMA EEHSEFVIGF ISGSRVSMKP EFLHLTPGVQ 430 440 450 460 470 480 LEAGGDNLGQ QYHSPQEVIG KRGSDIIIVG RGIIASANQL EAAKMYRKAA WEAYLSRLAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the cDNA encoding bovine uridine monophosphate synthase." Schoeber S., Detlef S., Schwenger B. Gene 124:307-308(1993) [PubMed: 8444356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"DUMPS cattle carry a point mutation in the uridine monophosphate synthase gene." Schwenger B., Schoeber S., Simon D. Genomics 16:241-244(1993) [PubMed: 8486364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65125 mRNA. Translation: CAA46253.1. BC112872 mRNA. Translation: AAI12873.1.
IPI	IPI00710267.
PIR	JN0558.
RefSeq	NP_803474.1.
UniGene	Bt.2379
3D structure databases
HSSP	HSSP built from PDB template 1DQW based on UniProtKB P03962.
SMR	P31754. Positions 222-479.
ModBase	Search...
Protein-protein interaction databases
STRING	P31754.
Proteomic databases
PRIDE	P31754.
Genome annotation databases
Ensembl	ENSBTAT00000018248; ENSBTAP00000018248; ENSBTAG00000013727; Bos taurus. [Genome view]
GeneID	281568.
KEGG	bta:281568.
Organism-specific databases
CTD	281568.
Phylogenomic databases
HOVERGEN	P31754.
Enzyme and pathway databases
BRENDA	2.4.2.10. 251. 4.1.1.23. 251.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase_1_core. IPR018089. OMPdecase_AS. IPR001754. OMPdecase_core. IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00215. OMPdecase. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit. TIGR01740. pyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYR5_BOVIN

Accession

Primary (citable) accession number: P31754
Secondary accession number(s): Q2KHV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	September 1, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)