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P31752

- ASNS_ASPOF

UniProt

P31752 - ASNS_ASPOF

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Protein
Asparagine synthetase [glutamine-hydrolyzing]
Gene
N/A
Organism
Asparagus officinalis (Garden asparagus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei98 – 981Glutamine By similarity
Binding sitei231 – 2311ATP; via carbonyl oxygen By similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC

GO - Biological processi

  1. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Short name:
AS
OrganismiAsparagus officinalis (Garden asparagus)
Taxonomic identifieri4686 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesAsparagaceaeAsparagoideaeAsparagus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 590589Asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056920Add
BLAST

Proteomic databases

PRIDEiP31752.

Expressioni

Developmental stagei

Levels of AS increase markedly after harvest.

Structurei

3D structure databases

ProteinModelPortaliP31752.
SMRiP31752. Positions 1-517.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2
Add
BLAST
Domaini193 – 516324Asparagine synthetase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine binding By similarity
Regioni75 – 773Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31752-1 [UniParc]FASTAAdd to Basket

« Hide

MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLCQ HGDCFLSHQR    50
LAIIDPASGD QPLYNEDKSI VVTVNGEIYN HEELRRRLPD HKYRTGSDCE 100
VIAHLYEEHG EDFVDMLDGM FSFVLLDTRN NCFVAARDAV GITPLYIGWG 150
LDGSVWLSSE MKGLNDDCEH FEVFPPGNLY SSRSGSFRRW YNPQWYNETI 200
PSAPYDPLVL RKAFEDAVIK RLMTDVPFGV LLSGGLDSSL VAAVTARHLA 250
GSKAAEQWGT QLHSFCVGLE GSPDLKAAKE VAEYLGTVHH EFHFTVQDGI 300
DAIEDVIFHI ETYDVTTIRA STPMFLMARK IKSLGVKMVI SGEGSDEIFG 350
GYLYFHKAPN KEEFHHETCR KIKALHQYDC LRANKATSAW GLEARVPFLD 400
KEFMDVAMSI DPESKMIKPD LGRIEKWVLR KAFDDEENPY LPKHILYRQK 450
EQFSDGVGYS WIDGLKAHAA KHVTDRMMLN AARIYPHNTP TTKEAYYYRM 500
IFERFFPQNS ARFTVPGGPS IACSTAKAIE WDARWSNNLD PSGRAALGVH 550
DSAYDPPLPS SISAGKGAAM ITNKKPRIVD VATPGVVIST 590
Length:590
Mass (Da):66,176
Last modified:January 23, 2007 - v2
Checksum:i9F7CA48BFE0CA712
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti513 – 5131F → L in CAA67889. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67958 mRNA. Translation: CAA48141.1.
X99552 Genomic DNA. Translation: CAA67889.1.
PIRiS25165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X67958 mRNA. Translation: CAA48141.1 .
X99552 Genomic DNA. Translation: CAA67889.1 .
PIRi S25165.

3D structure databases

ProteinModelPortali P31752.
SMRi P31752. Positions 1-517.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Proteomic databases

PRIDEi P31752.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of a cDNA clone for a harvest-induced asparagine synthetase from Asparagus officinalis L."
    Davies K.M., King G.A.
    Plant Physiol. 102:1337-1340(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Limbras 10.
    Tissue: Spear tip.
  2. "Nucleotide sequence of the asparagine synthetase gene from Asparagus officinalis L."
    Moyle R.L., Davies K.M., King G.A., Farnden K.J.F.
    Plant Gene Register PGR96-096
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiASNS_ASPOF
AccessioniPrimary (citable) accession number: P31752
Secondary accession number(s): Q96231
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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