P31752 (ASNS_ASPOF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Asparagine synthetase [glutamine-hydrolyzing] Short name=AS EC=6.3.5.4
Organism	Asparagus officinalis (Garden asparagus)
Taxonomic identifier	4686 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Asparagales › Asparagaceae › Asparagoideae › Asparagus

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
Developmental stage	Levels of AS increase markedly after harvest.
Sequence similarities	Contains 1 asparagine synthetase domain. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 590	589	Asparagine synthetase [glutamine-hydrolyzing]		PRO_0000056920
Regions
	Domain	2 – 185	184	Glutamine amidotransferase type-2
	Domain	193 – 516	324	Asparagine synthetase
Sites
	Active site	2	1	For GATase activity By similarity
Experimental info
	Sequence conflict	513	1	F → L in CAA67889. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P31752 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9F7CA48BFE0CA712	FASTA	590	66,176
	10 20 30 40 50 60 MCGILAVLGC SDDSQAKRVR VLELSRRLKH RGPDWSGLCQ HGDCFLSHQR LAIIDPASGD 70 80 90 100 110 120 QPLYNEDKSI VVTVNGEIYN HEELRRRLPD HKYRTGSDCE VIAHLYEEHG EDFVDMLDGM 130 140 150 160 170 180 FSFVLLDTRN NCFVAARDAV GITPLYIGWG LDGSVWLSSE MKGLNDDCEH FEVFPPGNLY 190 200 210 220 230 240 SSRSGSFRRW YNPQWYNETI PSAPYDPLVL RKAFEDAVIK RLMTDVPFGV LLSGGLDSSL 250 260 270 280 290 300 VAAVTARHLA GSKAAEQWGT QLHSFCVGLE GSPDLKAAKE VAEYLGTVHH EFHFTVQDGI 310 320 330 340 350 360 DAIEDVIFHI ETYDVTTIRA STPMFLMARK IKSLGVKMVI SGEGSDEIFG GYLYFHKAPN 370 380 390 400 410 420 KEEFHHETCR KIKALHQYDC LRANKATSAW GLEARVPFLD KEFMDVAMSI DPESKMIKPD 430 440 450 460 470 480 LGRIEKWVLR KAFDDEENPY LPKHILYRQK EQFSDGVGYS WIDGLKAHAA KHVTDRMMLN 490 500 510 520 530 540 AARIYPHNTP TTKEAYYYRM IFERFFPQNS ARFTVPGGPS IACSTAKAIE WDARWSNNLD 550 560 570 580 590 PSGRAALGVH DSAYDPPLPS SISAGKGAAM ITNKKPRIVD VATPGVVIST

References

[1]	"Isolation and characterization of a cDNA clone for a harvest-induced asparagine synthetase from Asparagus officinalis L." Davies K.M., King G.A. Plant Physiol. 102:1337-1340(1993) [PubMed: 7904077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Limbras 10. Tissue: Spear tip.
[2]	"Nucleotide sequence of the asparagine synthetase gene from Asparagus officinalis L." Moyle R.L., Davies K.M., King G.A., Farnden K.J.F. Plant Gene Register PGR96-096 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67958 mRNA. Translation: CAA48141.1. X99552 Genomic DNA. Translation: CAA67889.1.
PIR	S25165.
3D structure databases
ProteinModelPortal	P31752.
SMR	P31752. Positions 1-517.
ModBase	Search...
Protein family/group databases
MEROPS	C44.974.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006426. Asn_synth_AEB. IPR001962. Asn_synthase. IPR017932. GATase_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00733. Asn_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001589. Asn_synthetase_glu-h. 1 hit.
TIGRFAMs	TIGR01536. Asn_synth_AEB. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNS_ASPOF

Accession

Primary (citable) accession number: P31752
Secondary accession number(s): Q96231

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	October 19, 2011
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program