P31748 (AKT_MLVAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AKT kinase-transforming protein
EC=2.7.11.1

Gene names

Name:

V-AKT

Organism

AKT8 murine leukemia virus

Taxonomic identifier

11790 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Mammalian virus group › Replication competent viruses › Murine leukemia virus › unclassified Murine leukemia virus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with mouse THEM4. Ref.2
Domain	The AGC-kinase C-terminal mediates interaction with THEM4.
Post-translational modification	Autophosphorylated on threonine and serine residues.
Miscellaneous	This protein is synthesized as a Gag-Akt polyprotein.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Host-virus interaction
Disease	Oncogene
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	AKT kinase-transforming protein		PRO_0000085614
Regions
	Domain	26 – 129	104	PH
	Domain	171 – 429	259	Protein kinase
	Domain	430 – 501	72	AGC-kinase C-terminal
	Nucleotide binding	177 – 185	9	ATP By similarity
Sites
	Active site	295	1	Proton acceptor By similarity
	Binding site	200	1	ATP By similarity
Amino acid modifications
	Modified residue	347	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31748 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 5AEFDE58CD42F773	FASTA	501	57,870
	10 20 30 40 50 60 AREETLIIIP GLPLSLGATD TMNDVAIVKE GWLHKRGEYI KTWRPRYFLL KNDGTFIGYK 70 80 90 100 110 120 ERPQDVDQRE SPLNNFSVAQ CQLMKTERPR PNTFIIRCLQ WTTVIERTFH VETPEEREEW 130 140 150 160 170 180 ATAIQTVADG LKRQEEETMD FRSGSPSDNS GAEEMEVSLA KPKHRVTMNE FEYLKLLGKG 190 200 210 220 230 240 TFGKVILVKE KATGRYYAMK ILKKEVIVAK DEVAHTLTEN RVLQNSRHPF LTALKYSFQT 250 260 270 280 290 300 HDRLCFVMEY ANGGELFFHL SRERVFSEDR ARFYGAEIVS ALDYLHSEKN VVYRDLKLEN 310 320 330 340 350 360 LMLDKDGHIK ITDFGLCKEG IKDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM 370 380 390 400 410 420 YEMMCGRLPF YNQDHEKLFE LILMEEIRFP RTLGPEAKSL LSGLLKKDPT QRLGGGSEDA 430 440 450 460 470 480 KEIMQHRFFA NIVWQDVYEK KLSPPFKPQV TSETDTRYFD EEFTAQMITI TPPDQDDSME 490 500 CVDSERRPHF PQFSYSASGT A

References

[1]	"A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region." Bellacosa A., Testa J.R., Staal S.P., Tsichlis P.N. Science 254:274-277(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane." Maira S.-M., Galetic I., Brazil D.P., Kaech S., Ingley E., Thelen M., Hemmings B.A. Science 294:374-380(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH THEM4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80675 Genomic DNA. Translation: AAA42545.1.
3D structure databases
ProteinModelPortal	P31748.
SMR	P31748. Positions 24-142, 165-498.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR000961. AGC-kinase_C. IPR011009. Kinase-like_dom. IPR011993. PH_like_dom. IPR017892. Pkinase_C. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKT_MLVAT

Accession

Primary (citable) accession number: P31748

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program