P31717 (NMT1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycylpeptide N-tetradecanoyltransferase 1
EC=2.3.1.97

Alternative name(s):
Myristoyl-CoA:protein N-myristoyltransferase 1
Short name=NMT 1
Short name=Type I N-myristoyltransferase
Peptide N-myristoyltransferase 1

Gene names

Name:	NMT1
Synonyms:	NMT

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	May be associated with other proteins.
Subcellular location	Cytoplasm.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 497	497	Glycylpeptide N-tetradecanoyltransferase 1		PRO_0000064220
Regions
	Compositional bias	55 – 67	13	Poly-Lys
Amino acid modifications
	Modified residue	31	1	Phosphoserine By similarity
	Modified residue	47	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	114 – 115	2	KR → AK AA sequence Ref.2
	Sequence conflict	122	1	T → V AA sequence Ref.2
	Sequence conflict	168 – 169	2	VL → LF AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P31717 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 701A5C0B3554B037	FASTA	497	56,919
	10 20 30 40 50 60 MGDESETAVK PPAPPLPQMM EGNGNGHEHC SDCENEEDNS YNRGGLSPAN DTGAKKKKKK 70 80 90 100 110 120 QKKKKEKGSE TDSAQDQPVK MNSLPAERIQ EIQKAIELFS VGQGPAKTME EASKRSYQFW 130 140 150 160 170 180 DTQPVPKLGE VVNTHGPVEP DKDNIRQEPY TLPQGFTWDA LDLGDRGVLK ELYTLLNENY 190 200 210 220 230 240 VEDDDNMFRF DYSPEFLLWA LRPPGWLPQW HCGVRVVSSR KLVGFISAIP ANIHIYDTEK 250 260 270 280 290 300 KMVEINFLCV HKKLRSKRVA PVLIREITRR VHLEGIFQAV YTAGVVLPKP VGTCRYWHRS 310 320 330 340 350 360 LNPRKLIEVK FSHLSRNMTM QRTMKLYRLP ETPKTAGLRP MEKKDIPVVH QLLSRYLKQF 370 380 390 400 410 420 HLTPVMSQEE VEHWFYPQEN IIDTFVVENA NGEVTDFLSF YTLPSTIMNH PTHKSLKAAY 430 440 450 460 470 480 SFYNVHTQTP LLDLMSDALV LAKMKGFDVF NALDLMENKT FLEKLKFGIG DGNNLQYYLY 490 NWKCPSMGAE KVGLVLQ

References

[1]	"Bovine retina type I N-myristoyltransferase." Rundle D.R., Alvarez R.A., Anderson R.E. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Purification and partial sequencing of myristoyl-CoA:protein N-myristoyltransferase from bovine brain." McIlhinney R.A.J., McGlone K., Willis A.C. Biochem. J. 290:405-410(1993) [PubMed: 8452528] [Abstract] Cited for: PROTEIN SEQUENCE OF 95-104; 114-123; 168-191 AND 449-459. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF223384 mRNA. Translation: AAF31460.1.
IPI	IPI00723270.
PIR	S30363.
UniGene	Bt.88362.
3D structure databases
ProteinModelPortal	P31717.
SMR	P31717. Positions 155-497.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	maNOG13900.
HOVERGEN	HBG003404.
InParanoid	P31717.
OrthoDB	EOG4C5CJ6.
PhylomeDB	P31717.
Enzyme and pathway databases
BRENDA	2.3.1.97. 908.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. MyristoylCoA_TrFase. IPR022677. MyristoylCoA_TrFase_C. IPR022678. MyristoylCoA_TrFase_CS. IPR022676. MyristoylCoA_TrFase_N. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 2 hits.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT1_BOVIN

Accession

Primary (citable) accession number: P31717
Secondary accession number(s): Q9N177

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 11, 2001
Last modified:	November 16, 2011
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program