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Protein

DnaJ homolog subfamily A member 1

Gene

DNAJA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Co-chaperone for HSPA8/Hsc70 (PubMed:10816573). Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510). Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi134Zinc 1By similarity1
Metal bindingi137Zinc 1By similarity1
Metal bindingi150Zinc 2By similarity1
Metal bindingi153Zinc 2By similarity1
Metal bindingi177Zinc 2By similarity1
Metal bindingi180Zinc 2By similarity1
Metal bindingi193Zinc 1By similarity1
Metal bindingi196Zinc 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 205CR-typeAdd BLAST85

GO - Molecular functioni

  • ATP binding Source: InterPro
  • C3HC4-type RING finger domain binding Source: BHF-UCL
  • chaperone binding Source: UniProtKB
  • G-protein coupled receptor binding Source: ParkinsonsUK-UCL
  • Hsp70 protein binding Source: UniProtKB
  • low-density lipoprotein particle receptor binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • Tat protein binding Source: ParkinsonsUK-UCL
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

GO - Biological processi

  • androgen receptor signaling pathway Source: Ensembl
  • DNA damage response, detection of DNA damage Source: Ensembl
  • flagellated sperm motility Source: Ensembl
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of establishment of protein localization to mitochondrion Source: ParkinsonsUK-UCL
  • negative regulation of JUN kinase activity Source: UniProtKB
  • negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
  • negative regulation of protein ubiquitination Source: ParkinsonsUK-UCL
  • positive regulation of apoptotic process Source: UniProtKB
  • protein folding Source: ProtInc
  • protein localization to mitochondrion Source: UniProtKB
  • regulation of protein transport Source: UniProtKB
  • response to heat Source: InterPro
  • response to unfolded protein Source: ProtInc
  • spermatogenesis Source: Ensembl
  • toxin transport Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000086061-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily A member 1
Alternative name(s):
DnaJ protein homolog 2
HSDJ
Heat shock 40 kDa protein 4
Heat shock protein J2
Short name:
HSJ-2
Human DnaJ protein 2
Short name:
hDj-2
Gene namesi
Name:DNAJA1
Synonyms:DNAJ2, HDJ2, HSJ2, HSPF4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:5229. DNAJA1.

Subcellular locationi

  • Membrane 1 Publication; Lipid-anchor 1 Publication
  • Cytoplasm 1 Publication
  • Microsome By similarity
  • Nucleus 1 Publication
  • Cytoplasmperinuclear region 1 Publication
  • Mitochondrion By similarity

  • Note: Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.By similarity

GO - Cellular componenti

  • cytoplasmic side of endoplasmic reticulum membrane Source: ParkinsonsUK-UCL
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • mitochondrion Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi394C → S: Loss of farnesylation. 1 Publication1

Organism-specific databases

DisGeNETi3301.
OpenTargetsiENSG00000086061.
PharmGKBiPA31536.

Chemistry databases

ChEMBLiCHEMBL2189122.

Polymorphism and mutation databases

BioMutaiDNAJA1.
DMDMi1706474.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710081 – 394DnaJ homolog subfamily A member 1Add BLAST394
PropeptideiPRO_0000393941395 – 397Removed in mature formCurated3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66N6-acetyllysineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei335PhosphoserineCombined sources1
Modified residuei381PhosphotyrosineCombined sources1
Modified residuei394Cysteine methyl esterCurated1
Lipidationi394S-farnesyl cysteine2 Publications1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP31689.
PaxDbiP31689.
PeptideAtlasiP31689.
PRIDEiP31689.

PTM databases

iPTMnetiP31689.
PhosphoSitePlusiP31689.
SwissPalmiP31689.

Expressioni

Tissue specificityi

Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.2 Publications

Inductioni

Up-regulated by heat shock.1 Publication

Gene expression databases

BgeeiENSG00000086061.
CleanExiHS_DNAJA1.
GenevisibleiP31689. HS.

Organism-specific databases

HPAiHPA001306.

Interactioni

Subunit structurei

Identified in a complex with HSPA1B and BAX (PubMed:14752510). Interacts with RNF207 (PubMed:25281747).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AICDAQ9GZX76EBI-347834,EBI-3834328

GO - Molecular functioni

  • C3HC4-type RING finger domain binding Source: BHF-UCL
  • chaperone binding Source: UniProtKB
  • G-protein coupled receptor binding Source: ParkinsonsUK-UCL
  • Hsp70 protein binding Source: UniProtKB
  • low-density lipoprotein particle receptor binding Source: MGI
  • Tat protein binding Source: ParkinsonsUK-UCL
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi109534. 155 interactors.
IntActiP31689. 83 interactors.
MINTiMINT-5004184.
STRINGi9606.ENSP00000369127.

Chemistry databases

BindingDBiP31689.

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Helixi19 – 33Combined sources15
Helixi35 – 37Combined sources3
Beta strandi39 – 41Combined sources3
Helixi42 – 54Combined sources13
Helixi58 – 64Combined sources7
Turni65 – 67Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LO1NMR-A1-70[»]
2M6YNMR-A1-67[»]
ProteinModelPortaliP31689.
SMRiP31689.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 68JAdd BLAST63
Repeati134 – 141CXXCXGXG motif8
Repeati150 – 157CXXCXGXG motif8
Repeati177 – 184CXXCXGXG motif8
Repeati193 – 200CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 96Gly-richAdd BLAST22

Sequence similaritiesi

Contains 1 CR-type zinc finger.Curated
Contains 1 J domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 205CR-typeAdd BLAST85

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0712. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiP31689.
KOiK09502.
OMAiIIEFRVI.
OrthoDBiEOG091G0CAC.
PhylomeDBiP31689.
TreeFamiTF105141.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P31689-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVKETTYYDV LGVKPNATQE ELKKAYRKLA LKYHPDKNPN EGEKFKQISQ
60 70 80 90 100
AYEVLSDAKK RELYDKGGEQ AIKEGGAGGG FGSPMDIFDM FFGGGGRMQR
110 120 130 140 150
ERRGKNVVHQ LSVTLEDLYN GATRKLALQK NVICDKCEGR GGKKGAVECC
160 170 180 190 200
PNCRGTGMQI RIHQIGPGMV QQIQSVCMEC QGHGERISPK DRCKSCNGRK
210 220 230 240 250
IVREKKILEV HIDKGMKDGQ KITFHGEGDQ EPGLEPGDII IVLDQKDHAV
260 270 280 290 300
FTRRGEDLFM CMDIQLVEAL CGFQKPISTL DNRTIVITSH PGQIVKHGDI
310 320 330 340 350
KCVLNEGMPI YRRPYEKGRL IIEFKVNFPE NGFLSPDKLS LLEKLLPERK
360 370 380 390
EVEETDEMDQ VELVDFDPNQ ERRRHYNGEA YEDDEHHPRG GVQCQTS
Length:397
Mass (Da):44,868
Last modified:October 1, 1996 - v2
Checksum:iA899C06F6BB32780
GO
Isoform 2 (identifier: P31689-2) [UniParc]FASTAAdd to basket
Also known as: nDnaJA1

The sequence of this isoform differs from the canonical sequence as follows:
     327-397: NFPENGFLSP...PRGGVQCQTS → SCNVL

Show »
Length:331
Mass (Da):37,045
Checksum:iE3FFC159AA4BE7C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti274Q → H in BAA02656 (PubMed:8334161).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046566327 – 397NFPEN…QCQTS → SCNVL in isoform 2. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13388 mRNA. Translation: BAA02656.1.
L08069 mRNA. Translation: AAC37517.1.
AY186741 mRNA. Translation: AAO31694.1.
BT007292 mRNA. Translation: AAP35956.1.
AK289623 mRNA. Translation: BAF82312.1.
CH471071 Genomic DNA. Translation: EAW58525.1.
CH471071 Genomic DNA. Translation: EAW58526.1.
BC008182 mRNA. Translation: AAH08182.1.
CCDSiCCDS6533.1. [P31689-1]
PIRiS34630.
RefSeqiNP_001530.1. NM_001539.3. [P31689-1]
UniGeneiHs.445203.
Hs.707338.

Genome annotation databases

EnsembliENST00000330899; ENSP00000369127; ENSG00000086061. [P31689-1]
GeneIDi3301.
KEGGihsa:3301.
UCSCiuc003zsd.2. human. [P31689-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13388 mRNA. Translation: BAA02656.1.
L08069 mRNA. Translation: AAC37517.1.
AY186741 mRNA. Translation: AAO31694.1.
BT007292 mRNA. Translation: AAP35956.1.
AK289623 mRNA. Translation: BAF82312.1.
CH471071 Genomic DNA. Translation: EAW58525.1.
CH471071 Genomic DNA. Translation: EAW58526.1.
BC008182 mRNA. Translation: AAH08182.1.
CCDSiCCDS6533.1. [P31689-1]
PIRiS34630.
RefSeqiNP_001530.1. NM_001539.3. [P31689-1]
UniGeneiHs.445203.
Hs.707338.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LO1NMR-A1-70[»]
2M6YNMR-A1-67[»]
ProteinModelPortaliP31689.
SMRiP31689.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109534. 155 interactors.
IntActiP31689. 83 interactors.
MINTiMINT-5004184.
STRINGi9606.ENSP00000369127.

Chemistry databases

BindingDBiP31689.
ChEMBLiCHEMBL2189122.

PTM databases

iPTMnetiP31689.
PhosphoSitePlusiP31689.
SwissPalmiP31689.

Polymorphism and mutation databases

BioMutaiDNAJA1.
DMDMi1706474.

Proteomic databases

EPDiP31689.
PaxDbiP31689.
PeptideAtlasiP31689.
PRIDEiP31689.

Protocols and materials databases

DNASUi3301.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000330899; ENSP00000369127; ENSG00000086061. [P31689-1]
GeneIDi3301.
KEGGihsa:3301.
UCSCiuc003zsd.2. human. [P31689-1]

Organism-specific databases

CTDi3301.
DisGeNETi3301.
GeneCardsiDNAJA1.
H-InvDBHIX0007975.
HGNCiHGNC:5229. DNAJA1.
HPAiHPA001306.
MIMi602837. gene.
neXtProtiNX_P31689.
OpenTargetsiENSG00000086061.
PharmGKBiPA31536.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0712. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00860000133716.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiP31689.
KOiK09502.
OMAiIIEFRVI.
OrthoDBiEOG091G0CAC.
PhylomeDBiP31689.
TreeFamiTF105141.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000086061-MONOMER.

Miscellaneous databases

GeneWikiiDNAJA1.
GenomeRNAii3301.
PROiP31689.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000086061.
CleanExiHS_DNAJA1.
GenevisibleiP31689. HS.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJA1_HUMAN
AccessioniPrimary (citable) accession number: P31689
Secondary accession number(s): Q5T7Q0, Q86TL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 182 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.