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Protein

Trehalose-phosphatase

Gene

TPS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphatase catalytic subunit of the trehalose synthase complex that catalyzes the production of trehalose from glucose-6-phosphate and UDP-alpha-D-glucose in a two step process.1 Publication

Miscellaneous

Present with 22700 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Trehalose 6-phosphate + H2O = trehalose + phosphate.

Cofactori

Enzyme regulationi

Inhibited by EDTA.

Kineticsi

  1. KM=0.2 mM for trehalose 6-phosphate (at pH 6.0)1 Publication
  2. KM=0.5 mM for trehalose 6-phosphate (at pH 7.5)1 Publication
  1. Vmax=20 nmol/min/mg enzyme (at pH 6.0)1 Publication
  2. Vmax=10 nmol/min/mg enzyme (at pH 7.5)1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

GO - Molecular functioni

  • trehalose-phosphatase activity Source: SGD

GO - Biological processi

  • cellular response to heat Source: SGD
  • trehalose biosynthetic process Source: SGD
  • trehalose metabolism in response to stress Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processStress response

Enzyme and pathway databases

BioCyciMetaCyc:YDR074W-MONOMER
YEAST:YDR074W-MONOMER
BRENDAi2.4.1.15 984

Protein family/group databases

CAZyiGT20 Glycosyltransferase Family 20

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-phosphatase (EC:3.1.3.12)
Alternative name(s):
Trehalose synthase complex catalytic subunit TPS2
Trehalose-6-phosphate phosphatase
Short name:
TPP
Gene namesi
Name:TPS2
Synonyms:PFK3
Ordered Locus Names:YDR074W
ORF Names:YD8554.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR074W
SGDiS000002481 TPS2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001225091 – 896Trehalose-phosphataseAdd BLAST896

Proteomic databases

MaxQBiP31688
PaxDbiP31688
PRIDEiP31688

PTM databases

iPTMnetiP31688

Expressioni

Inductioni

By heat shock. Repressed by glucose.

Interactioni

Subunit structurei

The trehalose synthase complex is composed of the two catalytic subunits TPS1 and TPS2, and at least one of the two regulatory subunits TPS3 or TSL1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32129, 572 interactors
DIPiDIP-823N
IntActiP31688, 14 interactors
MINTiP31688
STRINGi4932.YDR074W

Structurei

3D structure databases

ProteinModelPortaliP31688
SMRiP31688
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 554GlycosyltransferaseAdd BLAST554

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 20 family.Curated
In the C-terminal section; belongs to the trehalose phosphatase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075394
HOGENOMiHOG000191477
InParanoidiP31688
KOiK16055
OMAiDSVCRDG
OrthoDBiEOG092C0LDC

Family and domain databases

CDDicd03788 GT1_TPS, 1 hit
Gene3Di3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR001830 Glyco_trans_20
IPR036412 HAD-like_sf
IPR006379 HAD-SF_hydro_IIB
IPR023214 HAD_sf
IPR003337 Trehalose_PPase
PfamiView protein in Pfam
PF00982 Glyco_transf_20, 1 hit
PF02358 Trehalose_PPase, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01484 HAD-SF-IIB, 1 hit
TIGR00685 T6PP, 1 hit

Sequencei

Sequence statusi: Complete.

P31688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTAQDNSP KKRQRIINCV TQLPYKIQLG ESNDDWKISA TTGNSALFSS
60 70 80 90 100
LEYLQFDSTE YEQHVVGWTG EITRTERNLF TREAKEKPQD LDDDPLYLTK
110 120 130 140 150
EQINGLTTTL QDHMKSDKEA KTDTTQTAPV TNNVHPVWLL RKNQSRWRNY
160 170 180 190 200
AEKVIWPTFH YILNPSNEGE QEKNWWYDYV KFNEAYAQKI GEVYRKGDII
210 220 230 240 250
WIHDYYLLLL PQLLRMKFND ESIIIGYFHH APWPSNEYFR CLPRRKQILD
260 270 280 290 300
GLVGANRICF QNESFSRHFV SSCKRLLDAT AKKSKNSSNS DQYQVSVYGG
310 320 330 340 350
DVLVDSLPIG VNTTQILKDA FTKDIDSKVL SIKQAYQNKK IIIGRDRLDS
360 370 380 390 400
VRGVVQKLRA FETFLAMYPE WRDQVVLIQV SSPTANRNSP QTIRLEQQVN
410 420 430 440 450
ELVNSINSEY GNLNFSPVQH YYMRIPKDVY LSLLRVADLC LITSVRDGMN
460 470 480 490 500
TTALEYVTVK SHMSNFLCYG NPLILSEFSG SSNVLKDAIV VNPWDSVAVA
510 520 530 540 550
KSINMALKLD KEEKSNLESK LWKEVPTIQD WTNKFLSSLK EQASSNDDME
560 570 580 590 600
RKMTPALNRP VLLENYKQAK RRLFLFDYDG TLTPIVKDPA AAIPSARLYT
610 620 630 640 650
ILQKLCADPH NQIWIISGRD QKFLNKWLGG KLPQLGLSAE HGCFMKDVSC
660 670 680 690 700
QDWVNLTEKV DMSWQVRVNE VMEEFTTRTP GSFIERKKVA LTWHYRRTVP
710 720 730 740 750
ELGEFHAKEL KEKLLSFTDD FDLEVMDGKA NIEVRPRFVN KGEIVKRLVW
760 770 780 790 800
HQHGKPQDML KGISEKLPKD EMPDFVLCLG DDFTDEDMFR QLNTIETCWK
810 820 830 840 850
EKYPDQKNQW GNYGFYPVTV GSASKKTVAK AHLTDPQQVL ETLGLLVGDV
860 870 880 890
SLFQSAGTVD LDSRGHVKNS ESSLKSKLAS KAYVMKRSAS YTGAKV
Length:896
Mass (Da):102,976
Last modified:October 1, 1996 - v3
Checksum:iE349672CCCDA9349
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48F → Y in CAA50025 (PubMed:8444170).Curated1
Sequence conflicti289N → D in CAA50025 (PubMed:8444170).Curated1
Sequence conflicti542Q → K in CAA50025 (PubMed:8444170).Curated1
Sequence conflicti546N → D in CAA50025 (PubMed:8444170).Curated1
Sequence conflicti549M → V in CAA50025 (PubMed:8444170).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70694 Genomic DNA Translation: CAA50025.1
Z46796 Genomic DNA Translation: CAA86796.1
Z74370 Genomic DNA Translation: CAA98893.1
X58858 Genomic DNA Translation: CAA41661.1
BK006938 Genomic DNA Translation: DAA11920.1
PIRiS48761
RefSeqiNP_010359.1, NM_001180382.1

Genome annotation databases

EnsemblFungiiYDR074W; YDR074W; YDR074W
GeneIDi851646
KEGGisce:YDR074W

Similar proteinsi

Entry informationi

Entry nameiTPS2_YEAST
AccessioniPrimary (citable) accession number: P31688
Secondary accession number(s): D6VS60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 177 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health