Reviewed, UniProtKB/Swiss-Prot P31686 (4CL1_SOYBN)

Names and origin

Protein names	Recommended name:     4-coumarate--CoA ligase 1       Short name=4CL 1     EC=6.2.1.12 Alternative name(s):     4-coumaroyl-CoA synthase 1     Clone 4CL14
Organism	Glycine max (Soybean)
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	293 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step 1/2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 293	›293	4-coumarate--CoA ligase 1		PRO_0000193038
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P31686-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 25868497FCC022EC	FASTA	293	32,027
	10 20 30 40 50 60 AKATILLMPK FDINSLLALI HKHKVTIAPV VPPIVLAISK SPDLHKYDLS SIRVLKSGGA 70 80 90 100 110 120 PLGKELEDTL RAKFPNAKLG QGYGMTEAGP VLTMSLAFAK EPIDVKPGAC GTVVRNAEMK 130 140 150 160 170 180 IVDPETGHSL PRNQSGEICI RGDQIMKGYL NDGEATERTI DKDGWLHTGD IGYIDDDDEL 190 200 210 220 230 240 FIVDRLKELI KYKGFQVAPA ELEALLLTHP KISDAAVVPM KDEAAGEVPV AFVVISNGYT 250 260 270 280 290 DTTEDEIKQF ISKQVVFYKR INRVFFIDAI PKSPSGKILR KDLRAKIAAS VPK

References

[1]

"Molecular cloning and expression of 4-coumarate:coenzyme A ligase, an enzyme involved in the resistance response of soybean (Glycine max L.) against pathogen attack." Uhlmann A., Ebel J. Plant Physiol. 102:1147-1156(1993) [PubMed: 8278545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Harosoy 63.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69954 mRNA. Translation: CAA49575.1.
PIR	S31705.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.2.1.12. 299.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL1_SOYBN

Accession

Primary (citable) accession number: P31686

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)