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Protein

Enolase

Gene

ENO

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+Note: Mg2+ is required for catalysis and for stabilizing the dimer.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. Pyruvate kinase (PYK2), Pyruvate kinase (PYK5), Pyruvate kinase (PYK4a), Pyruvate kinase (CHLRE_02g147900v5), Pyruvate kinase (PYK1), Pyruvate kinase (PYK3a|PYK3b|PYK3c), Pyruvate kinase (CHLRE_10g426292v5), Pyruvate kinase (PYK3a|PYK3b|PYK3c), Pyruvate kinase (CHLRE_02g147900v5), Pyruvate kinase (CHLRE_03g144847v5), Pyruvate kinase (CHLRE_02g147900v5), Pyruvate kinase (PYK3a|PYK3b|PYK3c), Pyruvate kinase (CHLRE_06g280950v5), Pyruvate kinase (CHLRE_02g147900v5), Pyruvate kinase (PYK6-5), Pyruvate kinase (CHLRE_05g234700v5)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95SubstrateBy similarity1
Binding sitei104SubstrateBy similarity1
Active sitei147Proton donorBy similarity1
Metal bindingi182MagnesiumBy similarity1
Metal bindingi232MagnesiumBy similarity1
Binding sitei232SubstrateBy similarity1
Metal bindingi257MagnesiumBy similarity1
Binding sitei257SubstrateBy similarity1
Active sitei282Proton acceptorBy similarity1
Binding sitei333SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000134068‹1 – 372EnolaseAdd BLAST›372

Proteomic databases

PRIDEiP31683
ProMEXiP31683

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP31683
SMRiP31683
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 312Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670 Eukaryota
COG0148 LUCA

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Fragment.

P31683-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
VTKAVENINA IIAPALKGMD PVKQAEIDQK MKDLDGTDNK GKLGANAILA
60 70 80 90 100
VSMAVCKAGA AEKGVPLYKH IADLAGNSKL ILPVPSFNII NGGSHAGNAL
110 120 130 140 150
AMQEFMILPV GASSFSEAMR MGCEVYHALK GLIKAKYGQD ACNVGDEGGF
160 170 180 190 200
APNIGSNDEG LNLVNEAIEK AGYTGKVKIG MDVASSEFYT EDGMYDLDFK
210 220 230 240 250
NQPNDGSQKK TKEQMLELYN EFCKKYPVIS IEDPFEQDDW EPCAKLTTEN
260 270 280 290 300
ICQVVGDDIL VTNPVRVKKA IDAKAVNALL LKVNQIGTIT ESIEAVRMAK
310 320 330 340 350
EAGWGVMTSH RSGETEDSFI ADLAVGLASG QIKTGAPCRS ERNAKYNQLL
360 370
RIEEELGENA VYAGESWRHI GW
Length:372
Mass (Da):40,110
Last modified:July 1, 1993 - v1
Checksum:iD7A04BA9D25B1058
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66412 mRNA Translation: CAA47043.1
PIRiS24996

Genome annotation databases

EnsemblPlantsiEDO96709; EDO96709; CHLREDRAFT_83064
GrameneiEDO96709; EDO96709; CHLREDRAFT_83064

Similar proteinsi

Entry informationi

Entry nameiENO_CHLRE
AccessioniPrimary (citable) accession number: P31683
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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