P31680 (DJLA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ-like protein DjlA

Gene names

Name:	djlA
Synonyms:	yabH
Ordered Locus Names:	b0055, JW0054

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory DnaK co-chaperone. Direct interaction between DnaK and DjlA is needed for the induction of the wcaABCDE operon, involved in the synthesis of a colanic acid polysaccharide capsule, possibly through activation of the RcsB/RcsC phosphotransfer signaling pathway. The colanic acid capsule may help the bacterium survive conditions outside the host. Ref.7 Ref.8
Subunit structure	Homodimer Potential.
Subcellular location	Cell inner membrane; Single-pass type III membrane protein Ref.8.
Domain	The transmembrane domain is a dimerization domain. Ref.9
Sequence similarities	Contains 1 J domain.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chaperone binding Inferred from electronic annotation. Source: InterPro heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	DnaJ-like protein DjlA HAMAP MF_01153		PRO_0000209424
Regions
	Topological domain	1 – 6	6	Periplasmic Potential
	Transmembrane	7 – 31	25	Helical HAMAP MF_01153
	Topological domain	32 – 271	240	Cytoplasmic HAMAP MF_01153
	Domain	205 – 271	67	J
Experimental info
	Mutagenesis	15	1	L → R: Loss of activation of rcs.
	Mutagenesis	16	1	M → R: Only partial activation of rcs.
	Mutagenesis	233	1	H → Q: Loss of activity. Ref.6
	Sequence conflict	195	1	Q → L in AAT42473. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P31680 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 80A0FC28F6D470DF	FASTA	271	30,579
	10 20 30 40 50 60 MQYWGKIIGV AVALLMGGGF WGVVLGLLIG HMFDKARSRK MAWFANQRER QALFFATTFE 70 80 90 100 110 120 VMGHLTKSKG RVTEADIHIA SQLMDRMNLH GASRTAAQNA FRVGKSDNYP LREKMRQFRS 130 140 150 160 170 180 VCFGRFDLIR MFLEIQIQAA FADGSLHPNE RAVLYVIAEE LGISRAQFDQ FLRMMQGGAQ 190 200 210 220 230 240 FGGGYQQQTG GGNWQQAQRG PTLEDACNVL GVKPTDDATT IKRAYRKLMS EHHPDKLVAK 250 260 270 GLPPEMMEMA KQKAQEIQQA YELIKQQKGF K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations." Lenski R.E., Winkworth C.L., Riley M.A. J. Mol. Evol. 56:498-508(2003) [PubMed: 12664169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 55-219. Strain: B.
[5]	"A novel DnaJ-like protein in Escherichia coli inserts into the cytoplasmic membrane with a type III topology." Clarke D.J., Jacq A., Holland I.B. Mol. Microbiol. 20:1273-1286(1996) [PubMed: 8809778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45, CHARACTERIZATION.
[6]	"Positive control of the two-component RcsC/B signal transduction network by DjIA: a member of the DnaJ family of molecular chaperones in Escherichia coli." Kelley W.L., Georgopoulos C. Mol. Microbiol. 25:913-931(1997) [PubMed: 9364917] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS OF HIS-233.
[7]	"Characterization of the RcsC->YojN->RcsB phosphorelay signaling pathway involved in capsular synthesis in Escherichia coli." Chen M.H., Takeda S., Yamada H., Ishii Y., Yamashino T., Mizuno T. Biosci. Biotechnol. Biochem. 65:2364-2367(2001) [PubMed: 11758943] [Abstract] Cited for: FUNCTION IN THE REGULATION OF THE RCS SYSTEM.
[8]	"DjlA is a third DnaK co-chaperone of Escherichia coli, and DjlA-mediated induction of colanic acid capsule requires DjlA-DnaK interaction." Genevaux P., Wawrzynow A., Zylicz M., Georgopoulos C., Kelley W.L. J. Biol. Chem. 276:7906-7912(2001) [PubMed: 11106641] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"The transmembrane domain of the DnaJ-like protein DjlA is a dimerisation domain." Toutain C.M., Clarke D.J., Leeds J.A., Kuhn J., Beckwith J., Holland I.B., Jacq A. Mol. Genet. Genomics 268:761-770(2003) [PubMed: 12655402] [Abstract] Cited for: TRANSMEMBRANE DOMAIN.
[10]	"Point mutations in the transmembrane domain of DjlA, a membrane-linked DnaJ-like protein, abolish its function in promoting colanic acid production via the Rcs signal transduction pathway." Clarke D.J., Holland I.B., Jacq A. Mol. Microbiol. 25:933-944(1997) [PubMed: 9364918] [Abstract] Cited for: MUTAGENESIS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73166.1. AP009048 Genomic DNA. Translation: BAB96623.1. AY625119 Genomic DNA. Translation: AAT42473.1.
PIR	G64726.
RefSeq	NP_414597.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P31680.
SMR	P31680. Positions 203-266.
ModBase	Search...
Protein-protein interaction databases
IntAct	P31680. 7 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000689; EBESCP00000000689; EBESCG00000000577. EBESCT00000017245; EBESCP00000016536; EBESCG00000016304.
GeneID	948992.
GenomeReviews	Gene locus JW0054 in contig AP009048_GR. Gene locus b0055 in contig U00096_GR.
KEGG	ecj:JW0054. eco:b0055.
PATRIC	32115209. VBIEscCol129921_0056.
Organism-specific databases
EchoBASE	EB1530.
EcoGene	EG11570. djlA.
Phylogenomic databases
eggNOG	COG1076.
GeneTree	EBGT00050000010193.
HOGENOM	HBG508481.
OMA	LRACWRM.
ProtClustDB	PRK09430.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11570-MONOMER.
Gene expression databases
Genevestigator	P31680.
Family and domain databases
HAMAP	MF_01153. DjlA. [Tree]
InterPro	IPR023749. DjlA. IPR001623. DnaJ_N. IPR007791. Heat_shock_DnaJ-like_DjlA. IPR003095. Hsp_DnaJ. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit.
KO	K05801.
Pfam	PF00226. DnaJ. 1 hit. PF05099. TerB. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DJLA_ECOLI

Accession

Primary (citable) accession number: P31680
Secondary accession number(s): Q6IU30

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	January 25, 2012
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program