Reviewed, UniProtKB/Swiss-Prot P31678 (OTSB_ECOLI)

Names and origin

Protein names

Recommended name:
    Trehalose-phosphate phosphatase
      Short name=TPP
    EC=3.1.3.12
Alternative name(s):
    Trehalose 6-phosphate phosphatase
    Trehalose-phosphatase
    Osmoregulatory trehalose synthesis protein B

Gene names

Name:	otsB
Ordered Locus Names:	b1897, JW1886

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Removes the phosphate from trehalose 6-phosphate to produce free trehalose By similarity.
Catalytic activity	Trehalose 6-phosphate + H2O = trehalose + phosphate.
Cofactor	Divalent cation By similarity.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Sequence similarities	Belongs to the trehalose phosphatase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	trehalose-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Trehalose-phosphate phosphatase		PRO_0000058100

Sequences

	Sequence		Length	Mass (Da)
	P31678-1 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 015E90EAD981B155	FASTA	266	29,175
	10 20 30 40 50 60 MTEPLTETPE LSAKYAWFFD LDGTLAEIKP HPDQVVVPDN ILQGLQLLAT ASDGALALIS 70 80 90 100 110 120 GRSMVELDAL AKPYRFPLAG VHGAERRDIN GKTHIVHLPD AIARDISVQL HTVIAQYPGA 130 140 150 160 170 180 ELEAKGMAFA LHYRQAPQHE DALMTLAQRI TQIWPQMALQ QGKCVVEIKP RGTSKGEAIA 190 200 210 220 230 240 AFMQEAPFIG RTPVFLGDDL TDESGFAVVN RLGGMSVKIG TGATQASWRL AGVPDVWSWL 250 260 EMITTALQQK RENNRSDDYE SFSRSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex." Kaasen I., McDougall J., Stroem A.R. Gene 145:9-15(1994) [PubMed: 8045430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12 / CSH7.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69160 Genomic DNA. Translation: CAA48912.1. U00096 Genomic DNA. Translation: AAC74967.1. AP009048 Genomic DNA. Translation: BAA15718.1.
PIR	I83401.
RefSeq	AP_002516.1. NP_416411.1.
3D structure databases
ModBase	Search...
2-D gel databases
SWISS-2DPAGE	P31678.
Genome annotation databases
GeneID	946406.
GenomeReviews	Gene locus JW1886 in contig AP009048_GR. Gene locus b1897 in contig U00096_GR.
KEGG	ecj:JW1886. eco:b1897.
Organism-specific databases
EchoBASE	EB1702.
EcoGene	EG11752. otsB.
CMR	Search...
Phylogenomic databases
HOGENOM	P31678.
OMA	P31678. VGGHGAE.
Enzyme and pathway databases
BioCyc	EcoCyc:TREHALOSEPHOSPHASYN-MON. MetaCyc:TREHALOSEPHOSPHASYN-MON.
Family and domain databases
InterPro	IPR006379. HAD-SF_hydro_IIB. IPR003337. Trehalose_PPase. [Graphical view]
Pfam	PF02358. Trehalose_PPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit. TIGR00685. T6PP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

OTSB_ECOLI

Accession

Primary (citable) accession number: P31678

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)