Sugar efflux transporter A - Escherichia coli (strain K12)

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P31675 (SETA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sugar efflux transporter A

Gene names

Name:	setA
Synonyms:	yabM
Ordered Locus Names:	b0070, JW0069

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport IPTG, lactose and glucose. Has broad substrate specificity, with preferences for glucosides or galactosides with alkyl or aryl substituents.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily. Set transporter family.

Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to stress Inferred from expression pattern. Source: EcoCyc glucose transport Inferred from direct assay Ref.6. Source: EcoliWiki lactose transport Inferred from direct assay Ref.6. Source: EcoliWiki
Cellular component	integral to membrane Inferred from direct assay Ref.5. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sugar efflux transmembrane transporter activity Inferred from direct assay Ref.6. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 392

392

Sugar efflux transporter A

PRO_0000209359

Regions

Topological domain

1 – 10

Cytoplasmic Potential

Transmembrane

11 – 31

Helical; Potential

Topological domain

32 – 48

Periplasmic Potential

Transmembrane

49 – 69

Helical; Potential

Topological domain

70 – 81

Cytoplasmic Potential

Transmembrane

82 – 102

Helical; Potential

Topological domain

103 – 106

Periplasmic Potential

Transmembrane

107 – 127

Helical; Potential

Topological domain

128 – 149

Cytoplasmic Potential

Transmembrane

150 – 170

Helical; Potential

Topological domain

171

Periplasmic Potential

Transmembrane

172 – 192

Helical; Potential

Topological domain

193 – 219

Cytoplasmic Potential

Transmembrane

220 – 240

Helical; Potential

Topological domain

241 – 251

Periplasmic Potential

Transmembrane

252 – 272

Helical; Potential

Topological domain

273 – 282

Cytoplasmic Potential

Transmembrane

283 – 303

Helical; Potential

Topological domain

304 – 308

Periplasmic Potential

Transmembrane

309 – 329

Helical; Potential

Topological domain

330 – 342

Cytoplasmic Potential

Transmembrane

343 – 363

Helical; Potential

Topological domain

364 – 365

Periplasmic Potential

Transmembrane

366 – 386

Helical; Potential

Topological domain

387 – 392

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P31675 [UniParc].

Last modified October 11, 2004. Version 3.
Checksum: 8CBD368BBC448460
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FASTA

392

42,713

        10         20         30         40         50         60 
MIWIMTMARR MNGVYAAFML VAFMMGVAGA LQAPTLSLFL SREVGAQPFW IGLFYTVNAI 

        70         80         90        100        110        120 
AGIGVSLWLA KRSDSQGDRR KLIIFCCLMA IGNALLFAFN RHYLTLITCG VLLASLANTA 

       130        140        150        160        170        180 
MPQLFALARE YADNSAREVV MFSSVMRAQL SLAWVIGPPL AFMLALNYGF TVMFSIAAGI 

       190        200        210        220        230        240 
FTLSLVLIAF MLPSVARVEL PSENALSMQG GWQDSNVRML FVASTLMWTC NTMYIIDMPL 

       250        260        270        280        290        300 
WISSELGLPD KLAGFLMGTA AGLEIPAMIL AGYYVKRYGK RRMMVIAVAA GVLFYTGLIF 

       310        320        330        340        350        360 
FNSRMALMTL QLFNAVFIGI VAGIGMLWFQ DLMPGRAGAA TTLFTNSIST GVILAGVIQG 

       370        380        390 
AIAQSWGHFA VYWVIAVISV VALFLTAKVK DV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract] Cited for: SEQUENCE REVISION TO 128.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 56. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The identification of a new family of sugar efflux pumps in Escherichia coli." Liu J.Y., Miller P.F., Gosink M., Olson E.R. Mol. Microbiol. 31:1845-1851(1999) [PubMed: 10209755] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Functional and biochemical characterization of Escherichia coli sugar efflux transporters." Liu J.Y., Miller P.F., Willard J., Olson E.R. J. Biol. Chem. 274:22977-22984(1999) [PubMed: 10438463] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAT48123.1. AP009048 Genomic DNA. Translation: BAB96639.2.
PIR	F64728.
RefSeq	YP_025293.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P31675.
ModBase	Search...
Protein family/group databases
TCDB	2.A.1.20.1. major facilitator superfamily (MFS).
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001424; EBESCP00000001424; EBESCG00000001186. EBESCT00000016733; EBESCP00000016024; EBESCG00000015792.
GeneID	944793.
GenomeReviews	Gene locus JW0069 in contig AP009048_GR. Gene locus b0070 in contig U00096_GR.
KEGG	ecj:JW0069. eco:b0070.
PATRIC	32115243. VBIEscCol129921_0073.
Organism-specific databases
EchoBASE	EB1704.
EcoGene	EG11754. setA.
Phylogenomic databases
eggNOG	COG0477.
GeneTree	EBGT00050000008827.
HOGENOM	HBG739814.
OMA	TMGRRLN.
PhylomeDB	P31675.
ProtClustDB	CLSK2304412.
Enzyme and pathway databases
BioCyc	EcoCyc:B0070-MONOMER.
Gene expression databases
Genevestigator	P31675.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004750. Sugar_efflux. [Graphical view]
KO	K03291.
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00899. 2A0120. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SETA_ECOLI

Accession

Primary (citable) accession number: P31675
Secondary accession number(s): P75639, Q6BF91

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents