Reviewed, UniProtKB/Swiss-Prot P31671 (GST28_FASHE)

Names and origin

Protein names	Recommended name:     Glutathione S-transferase class-mu 26 kDa isozyme 7       Short name=GST7     EC=2.5.1.18 Alternative name(s):     Fh7
Organism	Fasciola hepatica (Liver fluke)
Taxonomic identifier	6192 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Echinostomida › Echinostomata › Echinostomatoidea › Fasciolidae › Fasciola

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 218	217	Glutathione S-transferase class-mu 26 kDa isozyme 7		PRO_0000185809
Regions
	Domain	2 – 83	82	GST N-terminal
	Domain	85 – 203	119	GST C-terminal
Sites
	Active site	7	1	By similarity
Experimental info
	Sequence conflict	36 – 43	8	NDREKWLG → MIGRNGWA in CAA00119. Ref.3
	Sequence conflict	189 – 197	9	IKEYMKSER → SRYMSRA in CAA00119. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P31671-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 30D9EC6CDE6D592E	FASTA	218	25,327
	10 20 30 40 50 60 MPAKLGYWKI RGLQQPVRLF LEYLGEEYEE HLYGRNDREK WLGDKFNMGL DLPNLPYYID 70 80 90 100 110 120 DKCKLTQSVA IMRYIADKHG MLGSTPEERA RISMIEGAAM DLRIGFGLTC YNPKFEELKG 130 140 150 160 170 180 DYLKGLPTTL KMWSDFLGDR QYLIGSSVSH VDFMVYEALD CIRYLAPQCL DDFPKLKEFK 190 200 210 SRIEDLPKIK EYMKSERFIK WPLHSWTSPF GGGDAPPA

References

[1]	"Molecular characterization of cDNA sequences encoding glutathione S-transferases of Fasciola hepatica." Panaccio M., Wilson L.R., Crameri S.L., Wijffels G.L., Spithill T.W. Exp. Parasitol. 74:232-237(1992) [PubMed: 1740183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Erratum Panaccio M., Wilson L.R., Crameri S.L., Wijffels G.L., Spithill T.W. Exp. Parasitol. 77:385-385(1993) [PubMed: 8224094] [Abstract]
[3]	Crameri S. Patent number WO9008819, 09-AUG-1990 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-218.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M77680 mRNA. Translation: AAA29139.1. A00994 Unassigned RNA. Translation: CAA00119.1.
3D structure databases
HSSP	HSSP built from PDB template 1FHE based on UniProtKB P31670.
SMR	P31671. Positions 2-215.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.18. 39370.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST28_FASHE

Accession

Primary (citable) accession number: P31671

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	March 3, 2009
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)